慢性HBV感染者外周血单个核细胞CTLA-4基因多态性与病情转归的关联研究

慢性HBV感染者外周血单个核细胞CTLA一4 基因多态性与病情转归的关联研究木 张亚飞解俊侠郜玉峰沈继龙李旭 ·297· ·论 著· 【摘要】 目的初步探讨慢性HBV感染者外周血细胞毒性T淋巴细胞相关抗原-4(crnA-4)基因第1外显子区49 位基因单核苷酸多态性(singlenueleotidepolymorphism，SNP)与乙型肝炎病毒感染转归的关系。方法采用聚合酶链式反 应一限制性片段长度多态性法检测190例慢性HBV感染者和93例既往HBV感染者外周血CrnA-4基因49位点的多态 性。结果慢性HBV感染者c11A-4基因49位点MG基因型分布与对照组比较差异有显著性(P如．034)。慢性感染者G 等位基因频率明显低于对照组(0．561对0．677，P=O．008，OR=O．607o结论C，I．I^_4第1外显子49位基因多态性可能与乙 型肝炎病毒感染慢性化相关。 【关键词】乙型肝炎细胞毒性T淋巴细胞相关抗原-4单核苷酸多态性 StudyOnassociationbetweenthesinglenucleotidepolymorphismofcytotoxicTtrmphocyteassociatedagent 4gene旺伽1地gi蛐andoutcomeofpatientswi幽hepatitisvirtuBinfectionZHANGrdfei，XIEJunxia．GA0 yl咖昭，etaLDepartmentofInfectiousDisease，TheFirstHospitalAffdiatedtoAnhuiMedicalUnivers毋，日efei，Anhui， 23∞22 [Abstract】ObjectiveToinvestigatetheassociationbetweenthesinglenueleotidepolymorphismofcytotoxieT lymphocyteassociatedagent4(CTLA-4)geneexon1 region(+49)andtheoutcomeofpatientswithhepatitisvirusBinfec- tion．Me山odTherestrictionfragmentlengthpolymorphism-polymerasechainreactionw鹊usedtoanalyzetheC11．A—4 exon1+49polymorphismin190patientswithchronichepatitisBand93controlswithpastHBVinfection．ResultsThe frequencyofMGgenotypaatthe+49siteWaSstatisticallydifferentincontrolsandinchronichepatitisBpatients(P< 0．05)．ThefrequencyofGalleleatthe+49site哪statisticallylowerinchronichepatitisBpatientsthanincontrols (0．561侣0．677，P