配合力的计算(2)

Griffing提出的双列杂交共有4种方法，其中以方法4最常用。有5个亲本，即p=5,不包括自交和反交，共有组合数10个[a=p(p-1)/2]，完全随机区组，3次重复（b=3）。第一步，离差平方和的计算。校正值：C=X2../ab=294.22/30=2885.12总的St=∑X2ijk-C=3373.96-2885.12=488.84重复Sb=∑X2..k/a–C=(96.42102.3295.52)/10-2885.12=2.73一般配合力Sg=∑X2i../b(p-2)-4X2.../bp(p-2)=(102.72167297.52122.22992)/9-294.22/45=8075.15-7693.66=381.49特殊配合力Ss=∑∑X2ij./b-∑X2.j./b(p-2)2X2…/b(p-1)(p-2)=(30.9218.2229.5224.1243253.1240220.5215.8219.12)/3-(102.72167297.521222992)/9294.22/18=80.20机误Se=St-Sb-Sg-Ss=24.42父本母本⒈⒉345Xi..ⅠⅡⅢⅠⅡⅢⅠⅡⅢⅠⅡⅢⅠⅡⅢ110119.85.66.75.9911108.69.65.9102.7230.913141616.71917151312167318.24367.47.15.35.64.997.5429.55320.57.157122.2524.14015.819.199和588.4第二步，方差分析自由度平方和均方重复b-1=22.73Mb=1.37一般配合力p-1381.49Mg=95.37特殊配合力p(p-3)/2=580.2Ms=16.04试验误差(a-1)(b-1)=1824.42Me=1.36第三步，遗传参数估算σ2g=(Mg-Ms)/b(p-2)=8.81σ2s=(Ms-Me)/b=4.89两种配合力的相对重要性比较如下：一般配合力σ2g/σ2gσ2s=8.81/(8.814.89)=64.31%特殊配合力σ2s/σ2gσ2s=4.89/(8.814.89)=35.69一般配合力效应值计算如下：gˆ=(pXi.–2X..)/p(p-2)g1 =(5*34.2-196)/15=-1.67g2 =(5*55.6-196)/15=5.47g3 =(5*32.5-196)/15=-2.23g4 =(5*40.7-196)/15=0.5g5 =(5*33-196)/15=-2.07特殊配合力效应值计算如下：Sˆ=Xij–(Xi.X.j)/(p-2)2X../(p-1)(p-2)S1*2=10.3-(34.255.6)/398/6=-3.3S1*3=6.1-(34.232.5)/398/6=0.2S1*4=9.8-(34.240.7)/398/6=1.17S1*5=8-(34.233)/398/6=1.93S2*3=14.3-(55.632.5)/398/6=1.27S2*4=17.7-(55.640.7)/398/6=1.93S2*5=13.3-(55.633)/398/6=0.1S3*4=6.8-(32.540.7)/398/6=-1.27S3*5=5.3-(32.533)/398/6=-0.2S4*5=6.4-(40.733)/398/6=-1.83