植物组织中硝态氮的测定植物伤流液中无机磷含量的测定

植物组织中硝态氮的测定植物伤流液中无机磷含量的测定 植物中硝氮的定组组组组组组组组组【组组目的】流是植物根系主吸水的明,不同植物或同一 伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤植物在不同季的流度均不同,流度反根系强强 生理活弱和根系又吸收面大小。流除含有大量水分伤伤伤伤伤伤伤伤伤伤伤伤伤伤强 外,含有各无机及根部合成有机物包括植物伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 激素。硝氮是植物最主要的氮源。植物体内硝伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤氮含量往往能反映土壤中硝氮供情况,因此可作土壤伤伤伤伤伤伤伤伤伤伤伤伤伤伤肥氮肥的指。定植物体内的硝氮含量,不能伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤反映出植物的氮素情况,而且定蔬菜和植物原料伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤的加工制品的品也有重要的意。伤伤伤伤伤伤伤伤伤 【原理】硝氮与硝酸粉作用,生成粉色的偶氮伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤化合物,其色深浅与硝氮的度成正相,将品伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤示的色与准比色行比,可快速求得硝氮的伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤度。硝酸粉主要是由粉、檬酸、ɑ-伤伤伤伤伤伤伤伤伤胺、氨基苯磺酸混合而成,硝氮与硝酸粉反如下:伤伤伤伤伤伤伤伤伤伤伤伤-+-2+NO+H NO+Zn+HO322 ɑ-伤 伤胺重氮化合物-苯磺酸-偶氮-伤胺【材料】组组组组组 接骨木流液伤伤伤 【组组组组 】硝酸粉、伤伤伤50%醋酸、50mg/L氮流液 【】组组组 步在比色的五个孔中加入如表所示溶液:伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 伤孔号 目 123456-各管NO伤度(mg/mL)1020305060X3 432100蒸水，滴,伤伤伤伤伤 1234550mg/L硝氮液伤伤伤伤流液5，滴, 50%醋酸，滴,111111 111111硝酸粉，勺,伤伤伤伤伤 5min后即成5个不同度伤伤20、40、60、80、100 mg/mL硝氮的比色。再用伤伤伤伤伤伤伤伤伤6号孔中加入流液伤伤伤5滴及硝酸粉伤伤1勺,拌,伤伤伤5min后,将其所粉色伤伤伤伤与准比色比确定品液中硝氮的度。伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 【组组组组组果及理】 1.伤伤伤伤伤伤伤伤伤伤伤比色品溶液的色介于2~3之。伤伤 2.根据果,流液中硝氮的伤伤伤伤伤伤伤伤伤伤伤伤伤伤(10/5+20/10)/2=2mg/mL【】组组组 1.如若流液度高,超容量范可先将流液稀伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 伤伤伤伤伤伤一定倍数后在行滴加。 2.本中所的硝化氮的含量不准确,因其原伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 因又一下几点,首先是由于方法的原因致的,由于行比色伤伤伤伤伤伤伤伤伤伤 来定流液的度,因者不同,色差的辨伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 不同,很可能致偶人差。其次是由于流液在被伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 收集后是没有行密封的,里面的水分可能会一定伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 的散失,致所定的偏高。伤伤伤伤伤伤伤伤伤伤 方法的改:植物品硫酸伤伤伤伤伤伤伤伤伤+伤伤伤伤伤伤伤伤氧化氏法消煮后,用直接吸光光度法得伤伤[1] 硝氮伤伤(包括硝氮伤伤伤伤). 原理及方法如下: 硝氮在硫酸伤伤伤伤伤伤伤2伤伤伤伤伤伤伤伤伤伤伤氧化消煮的植物消煮液中, 硝氮和硝氮伤伤伤伤伤伤伤--以硝酸根离子(NO) 存在, 利用NO 在紫外光区220 nm伤有特征吸收峰, 可以直33 接定液的吸光度来定量硝氮。定伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤, 吸取5 mL 消煮液于50 mL 比色管中, 无氨水稀至刻度伤伤伤伤, 伤匀, 在波伤210 nm伤, 用1 cm石英比色皿, 以无氨水作参比, 在紫外分光光度行硝氮定。伤伤伤伤伤伤伤伤伤 3.通流法定植物中硝氮含量中,流法需要伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 收集流液,此消。伤伤伤伤伤伤伤伤伤伤伤伤伤 [1] 伤伤仙,葛,吴建之伤伤伤伤伤,常杰. 植物中硝氮、氨氮、氮定方伤伤伤伤伤伤伤伤伤伤伤伤法的比研究伤伤伤. 光学与光分析伤伤伤伤伤伤伤.Vol124 ,No12 ,pp2042206 植物中流液中氨基酸量的定组组组组组组组组组组组组—组组组组组三比色法【组组目的】根系是植物吸收水分和元素的主要器官,也是多有机 伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 物的合成和存器官,在流液中除含有离子外,含伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤有氨基酸、激素、糖、等有机物。定流液中氨基酸含量,伤伤伤伤伤伤伤伤伤伤伤伤可以了解根系活力弱。强 【组组原理】 伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤氨基酸与三乙醇溶液可生色反,生 成紫色化合物,色深浅与氨基酸含量成正比。伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 除脯氨酸与胺酸外,其他各氨基酸色的色与深伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤浅相差不大,因此利用此反可定量定流液中氨基酸的量。伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤【材料】组组组组组 接骨木流液伤伤伤 【】组组组组组 3%伤伤伤伤伤伤 三乙醇溶液 【操作方法】 1.去两只管按照下面号,并按照下，表二,行伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤加液,在沸水浴中加两份,取出察色是否化。伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 表二 组目管号 1 2 蒸水，滴,组组组组组 10 0 3%伤伤伤伤三溶液 5 5 伤流液，滴, 0 10【组组组果】一号管中色不, 伤伤伤伤伤2号管中加后由无色成紫色。伤伤伤伤伤伤伤伤伤伤伤流液中含有有理的氨基酸。 【组组组果】 1. 本只是定了流液中存在游离氨基酸,但伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 不能确定其含量,在确定流液中含有游离氨基酸伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤后用分光光度法定其中氨基酸的含量。伤伤伤伤伤伤伤伤伤伤伤 2.通本确定了一定流液中游离氨基酸的方法。伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 植物流液中无机磷含量的定组组组组组组组组组组组组 【目的】组组组组组 磷参与植物体内多伤伤伤伤伤伤伤伤伤伤伤伤代,促碳水化合物的合成,化和伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤运,施磷提高作物量和品有明 的效果。通本伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤掌握植物体内磷含量的定方法及其原理。 【原理】组组组组组 伤伤伤伤伤伤伤伤伤伤定磷含量的方法主要有磷比色法,可用于直接定植物中可溶伤伤伤伤伤伤伤伤伤伤伤伤伤伤性磷含量。 磷比色法:在伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤适宜的酸性条件下,磷酸能与酸作用伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤形成磷酸,并被抗坏血酸或化等原原,生成色的磷,并在伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤650nm伤伤伤伤又最大吸收峰,其色深浅与含磷量成正比,可直接比色定。伤伤伤 【组组材料】接骨木流液 伤伤伤 【组组组组】 10mg/L磷林液、磷伤伤伤伤 【组组方法】 1. 伤伤伤伤伤 准曲的制作 取6支伤伤伤伤伤伤伤管,分号0~5,按下表加入磷准溶液及其他,伤伤伤伤伤伤伤伤伤以制作准伤伤伤伤伤伤伤曲。另取1支伤伤伤伤管号6,作品管,按照下表加伤伤伤伤伤伤伤伤伤伤入各。并将各管伤伤伤伤伤伤伤伤伤伤伤伤伤充分匀,在45?水浴中保温25分,以伤伤伤伤伤伤伤空白照,在分光光伤650nm伤伤伤伤伤伤伤伤伤伤伤伤定吸光度。。以吸光度坐,磷含量横坐伤伤伤伤伤伤伤伤伤伤伤伤,制准曲。并根据6号管的吸光度,伤伤伤伤伤伤伤伤伤伤伤伤从准曲伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤上出流液中德可溶性磷的含量。 表三 伤管号 目 0 1 2 3 4 5 6 各管含磷量(ug) 0 2 4 6 8 10 X 10ug/L磷准溶液伤伤伤伤(mL) 0 0.2 0.4 0.6 0.8 1.0 伤品0.2蒸水，伤伤伤mL, 3.0 2.8 2.6 2.4 2.2 2.0 2.8定磷液，伤伤伤mL, 3.0 3.0 3.0 3.0 3.0 3.0 3.0吸光度，A,650 0 0.103 0.180 0.285 0.392 0.490 0.025 【组组组果】 1.根据果得出准伤伤伤伤伤伤伤伤伤伤曲 表四 伤伤准曲 22因伤R越接近1其偏差越小,本可得伤伤伤伤伤到R=0.9982很伤伤伤近于1,故伤伤准曲性伤伤伤伤伤伤伤伤伤相性良好。 2. 伤伤伤果 由Y=0.0485x 可知:6号管中的可溶性磷含量=0.250/0.0485=5.88ug. 【果】组组组组组 1. 本由于所取的接骨木流液中水分可能会有伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤伤 一定的失,致所的果偏大。伤伤伤伤伤伤伤伤伤伤伤伤伤