负载hTERT复制缺陷型腺病毒的包装及鉴定
负载hTERT复制缺陷型腺病毒的包装及鉴定
负载hTERT复制缺陷型腺病毒的包装及鉴定
负载hTERT复制缺陷型腺病毒的包装及鉴定-医学论文
作者:陈陵,向国春,李向红,蔡永国,李晶晶,房殿春,罗元辉,
李志宏,杨仕明 【关键词】 腺病毒表达载体 Package and
identification of replicationdeficient recombinant adenovirus expression vector of hTERT 【Abstract】 AIM: To construct a
replicationdeficient recombinant adenovirus expression vector of hTERT containing adenoviral genome. The adenovirus expression vector was then obtained and identified by infection test, electron microscopic observation and PCR coamplification. RESULTS: After purification and concintration, the titer of AdhTERT reached 5×1012 pfu/L. Virus particles could be found in HEK 293 cells under transmission electron microscope. Both adenovirus and hTERT special fragments could be amplified from AdhTERT by PCR, whereas hTERT special fragment could not be amplified from the control. CONCLUSION: The
replicationdeficient recombinant adenovirus expression vector of hTERT is succe{负载hTERT复制缺陷型腺病毒的包装及鉴定欢迎
您访问范,文,家}ssfully constructed. 【Keywords】 human
telomerase reverse transcriptase; adenovirus expression vector 【摘要】 目的: 构建负载人端粒酶逆转录酶Mr 127 000,
包含1132个氨基酸,其基因位于5号染色体短臂的最末端,在90%
以上的人类肿瘤中高表达,而正常成熟组织中则基本没有表达,1,,
因而hTERT是一很有前景的广谱、特异的抗肿瘤治疗靶点. 腺病毒载
体系统具有宿主范围广、感染率高、包装容量大、繁殖滴度高、不发
生整合及安全性好、性质稳定、载体制备较容易等特点, 目前已成为
继逆转录病毒载体后被广泛应用的载体系统,2,,可用作hTERT的异
位表达工具. 我们通过构建hTERT腺病毒表达载体,为进一步研究以
hTERT为靶点的肿瘤免疫治疗奠定基础.