微藻培养基

中国科学典型培养物保藏委员会淡水藻种库(FACHB)藻种信息 武汉武昌中科院水手生物研究所淡水藻种库邮编430072 TEL;027—87647715;FAX:027—87875132;EMALL:Irsong@ihb.ac.cn 藻种学名:Scenedesmus obliquus(Turp.)Kütz 中文译名:斜生栅藻 培养条件;温度25—28。C光照强度:2000LX 培养基(保种):SE 培养基成分: SE NaNO 3 25mg CaCl 2 2.5mg MgSO 4.7H 2 O 7.5mg K 2 HPO 4 7.5mg KH 2PO 4 17.5mg NaCl 2.5mg Soil extract 4 mL Fe-EDTA 0.1mL FeCl 3.6H 2 O 0.5mg A 5 solution 0.1mg Distil water 95.8mL A 5 solution: H 3BO 3 286 mg MnSO 4 .7H 2 O 250 mg MnCl 2.6H 2 O 181 mg ZnSO 4 .7H 2 O 22 mg CuSO 4.5H 2 O 7.4 mg Na 2 MnO 4 2.1 mg Distilled water 100 mL EDTA-Fe 配制方法:将EDTA和F eCl3分别溶于水和HCl(0.1N),混匀即可。 Na2-EDTA 1g Distilled water 50mg FeCl3.6H2O 81mg HCl(0.1N) 50mL Soil Extract 配制方法 取花园土(未施过肥)0.5kg 置于烧杯或三角瓶中,加入蒸馏水1000毫升,瓶口用透气塞封口,在水浴中加热2小时,冷却数小时,在无菌条件下过滤,取上清液,将灭菌蒸馏水加入上清液至总体积1000毫升。土壤提取液保存在4。C备用。 中国科学院典型培养物保藏委员会淡水藻种库(FACHB)藻种信息 武汉武昌中科院水手生物研究所淡水藻种库邮编430072 TEL;027—87647715;FAX:027—87875132;EMALL:Irsong@ihb.ac.cn 藻种学名: 中文译名: 培养条件;温度25。C光照强度:2000LX 培养基:HGZ 培养基成分: HGZ NaNO3 49.6mg K2HPO4 3.9mg MgSO4.7H2O 7.5mg CaCl2.2H2O 3.6mg Na2SiO3.9H2O 5.8mg PIV metal solution 0.3mL Soil extract 0.3mL Distilled water 99.4mL PH 8-8.5 CaCl2.2H2O and Na2SiO3.9H2O should be autoclaved separately 1)PIV metal solution FeCl3.6H2O 9.7mg MnCl2.4H2O 4.1mg ZnCl2 0.5mg CoCl2.6H2O 0.2mg Na2MoO4.2H2O 0.4mg Na2-EDTA 75mg Distilled water 100mL 2)Soil Extract(土壤提取液)配制方法 取花园土(未施过肥)0.5kg置于烧杯或三角瓶中,瓶口用透气塞封口,在水浴中加热2小时,冷却数小时,在无菌条件下过滤,取上清液,将灭菌蒸馏水加入上清液至总体积1000毫升。土壤提取液保存在4。C备用。 BG11培养基配方from Swede g/L 1.K2PO4.3H2O 0.04 2.MgSO4.7H2O 0.075 3.CaCl2.2H2O 0.36 Citric acid 0.006 4.Farric ammonium citrate 0.006 EDTA(di-sodium-magnes iumsalt) 0.001 5.Na2CO3 0.04 6.H3BO3 2.86 MnCl2.14H2O 1.81 ZnSO4.7H2O 0.222 Na2MoO4.2H2O 0.39 CuSO4.5H2O 0.079 Co(NO3)2.6H2O 0.494 7.NaNO3 1.5(weigh each time) Tomake one liter of medium : Put Sooinl of aqdist (Distilled water) in a flask, take 1ml of each of stock-solution 1-6 shake after each one and finally add alistilled water until the volume reaches one liter. HB111 318、190174、180、177、120、183、13、252、261、是此培养基 1、MgSO4.7H2O 12.5mg K2HPO47.5mg CaCO310mg Ferric citrate(1%) 0.05mL Citreic acid(1%) 0.05mL H2MoO4(1%) 0.025mL A5 solution 0.1mL 2、soil extract 2mL Distilled water 99.7mL A5 solution H3BO3286mg MnCl2.4H2O 181mg MnSO4.7H2O 250mg ZnSO4.7H2O 22mg CuSO4.5H2O 7.4mg Na2MoO4 2.1mg Distilled water 100mL