生远志及其皂苷与蜜远志对家兔离体肠平滑肌运动作用机制的实验研究

生远志及其皂苷与蜜远志对家兔离体肠平滑肌运动作用机制的实验研究 ÉúÔ?Ö???ÆäÔíÜÕÓëÃÛÔ?Ö??Ô?ÒÍÃÀëÌå??Æ?????ÔË??×?ÓÃ?úÖƵÄ浄 éÑÐ?? [ÂÛÎÄ×ÊÁÏ?âΪÄúËÑ??ÕûÀí] ×?Õß?º??ÜöÖñ Íõ?? ÎâêÍêÍ ÕÔº?Æ? Ìï?Õ Õ Åæ ???Ø?ü?Ê?? ÉúÔ?Ö???,,×ÜÔíÜÕ??,,ÃÛÔ?Ö???,,ÀëÌå???? ???ÕªÒª?ºÄ?µÄÌ?ÌÖÉúÔ?Ö???ÆäÔíÜÕÓëÃÛÔ?Ö??Ô?ÒÍÃÀëÌåÊ??þÖ???Æ??? ???µÄ×?ÓÃ?úÖÆ???????ÉÓÃÀëÌå????ʵÑé??ÓÃÉúÎï?úÄÜ?ÖÎöϵÍ??ÇÂ?ÉúÔ? Ö???ÆäÔíÜÕÓëÃÛÔ?Ö??ÔÕý???????????ßÒ?ÒÒõ?µ??î??×é????ÂÈ???µËùÖ ÂÀëÌå????ÊÕËõµÄÓ?Ïì???É??????µÄÕñ?ùÓëƵÂÊ???ÆËã?ö??ÒÖÖÆÂÊ???á?û ÉúÔ?Ö?ºÍÔ?Ö?ÔíÜÕ?ùÄÜÏÔÖøÒÖÖÆÕý??????ÔË????ÆäÒÖÖÆÂÊÏÔÖø?ßÓ ÚÃÛÔ?Ö?(P??0.01)??ÇÒÉúÔ?Ö?Óë×ÜÔíÜÕ??ÄÜÏÔÖøÒÖÖÆÒÒõ?µ??î??Ach ????×éÖ?????ÂÈ???µËùÖµÄ????ÊÕËõ????ÓëÃÛÔ?Ö?Ïà?ÈÓÐ??ÏÔÖø?îÒì(P? ?0.01)??ÃÛÔ?Ö??ÔÕý??????ºÍÂÈ???µËùÖµÄÀëÌå????ÐË?ÜÐÔÊÕËõÒ?ÓÐÒÖ ÖÆ×?ÓÃ(P??0.05) ???áÂÛÉúÔ?Ö???ÆäËùº?µÄ×ÜÔíÜÕ?Ô?ÒÍÃÀëÌå??Æ??? ??ÔË???ßÓÐÒÖÖÆ×?ÓÃ??ÆäÒÖÖÆ?úÖÆ???ÉÄÜϵÆäÓëÒÒõ?µ??î??×é???º Õù??×è?ÏM??H1ÊÜÌåÒÔ??Ö??ÓÒÖÖÆ????ÐË?ÜÓÐ?Ø??ÇÒÆäÒÖÖÆ×?ÓÃÊ Ç???ÉÄæµÄ??ÃÛÔ?Ö??ÔÕý??????ÔË??ÓÐÖ??ÓÒÖÖÆ×?ÓÃ??µ?ÏÔÖøÐ?ÓÚÉ úÔ?Ö???ÆäÔíÜÕ?? ?????Ø?ü?Ê?ºÉúÔ?Ö??? ×ÜÔíÜÕ?? ÃÛÔ?Ö??? ÀëÌå???? Crude Radix Polygalae and its Saponin and Honey-stir-????Experimental Research of baked Radix Polygalae on the ex vitro Intestine Movement Mechanism to Domestic Rabbit ????Abstract:ObjectiveTo approach the Crude Radix Polygalae and its saponin and Honey-stir- baked Radix Polygalae mechanism of in vitro intestine movement of domestic rabbit.MethodsUsed the in vitro intestine movement experiment, with the biologic function analyzer to record the influenses on ex- intestines and the intestines which contract were induced by Ach, Histamine and Barium Chloride; Got the ex- intestines amplitude and the frequency, and calculated the inhibition ratio.ResultsCompared with the control, the Crude Radix Polygalae and its saponin could all remarkably inhibit the exintestines' movements, the inhibition ratios were remarkably larger than that of Honey-stir- baked Radix Polygalae(P??0.01); and the Crude Radix Polygalae and its saponin could all remarkably inhibit the movements of the intestines which contract were resulted with Ach, Histamine and Barium Chloride; the inhibition ratios were remarkably larger than Honey-stir-baked Radix Polygalae(P??0.01); Honey-stir- baked Radix Polygalae also inhibited the excitability contract with the exintestines and the intestines which contract were induced by the Barium Chloride (P??0.05).Conclusion?ÙCrude Radix Polygalae and its saponin can inhibit the ex vitro intestine's movement of the domestic rabbit;the mechanism is possibly related with two factors, the one is Crude Radix Polygalae and its saponin compete with Ach and Histamine and block M, H1 receptor; the next is direct inhibition of ex vitro intestine excitability, and the inhibitory action is inreversible.?ÚHoney-stir- baked Radix Polygalae can directly inhibit the ex- intestine movement but the inhibitory action is remarkably less than Crude Radix Poly galae and its saponin. ????Key words:Crude Radix Polygalae; Saponin; Honey-stir- baked Radix Polygalae; Ex vitr intestine ????Ô?Ö?ϵÁÙ????ÓÃÖÐÒ?????ÓÃÓÚ??Éñ??ìî̵??Ö??ȵÈ??µ?ÒÑÓÐÎÄÏ×ÖÐÏÊ?û?ÔÆä??ÐÔµÄ??µÀ??Ç?ÆÚÑÐ????ÏÖÔ?Ö??ÔÎ???ÔË??ÓÐÃ?ÏÔÒÖÖÆ×?ÓÃ????ÐÔ?Ï?ó??Í?Ê???ÏÖÃÛÔ?Ö??ÔÎ???ÔË??µÄÒÖÖÆ×?ÓÃÐ?ÓÚÉúÔ?Ö???ÇÒ??ÐÔ?ÏÐ????ùÓÚÉÏÊöÑÐ???á?û???ÃÑÐ??Äâ???ìÉúÔ?Ö???ÆäÔíÜÕÓëÃÛÔ?Ö??Ô?ÒÍÃÀëÌåÊ??þÖ???Æ?????ÔË??µÄÓ?Ïì??ÒÔÌ?ÌÖÔ?Ö?ÒÖÖÆÎ???ÔË??µÄ×?ÓÃ?úÀí?? ????1 ?ÄÁÏÓë???? ????1.1 Ò??Ä?????ìÒ?ÖÆ?? ????1.1.1 ÉúÔ?Ö??ºÓÚ?É??ÊÐÎå?éÊ?ÖÐÒ??ÄÊÐ?????- ?Ð?Â?ÏÈÃ??ÌÊÚ?ø??ΪPolygala tenuifolia ?Willd.µÄ?ù??ϵÒ?µäÔØÕýÆ?Ò??Ä ?? ????1.1.2 ?äÃÛËÄ??ÄÏ???ä?úÆ?ÓÐÏÞ??Ë??öÆ????úÆ??ê×?ºÅ?ºQ/20276901-0?? ????1.1.3 ÃÛÔ?Ö?ϵÓÅ??Éè?Æ???áºÏ??ÐÔ??ÐÔÓëÎ?????ÐÔÒÔ??Ïà?ØÖ?ÒªÒ?Ð?Ñ?ʵÑé??É?Ñ??öµÄ×îÓÅÃÛÖÆÔ?Ö???ÒÕÖÆ???ø?É??ÓÉ?É??ÖÐÒ?Ò??óÑ?ÅÚÖÆ?ÌÑÐÊÒÌá???? ????1.1.4 ???ìÒ?ÒºÖÆ???Ö?ð?ÆÈ?ÉúÔ?Ö?100 g??µÈÉúÒ?Á?ÃÛÔ?Ö?119 g????ʵ?ÊÉúÔ?Ö?Á?Ϊ100 g???äÃÛÁ?19 g??ºÏ?Æ119 g??ÆäºóʵÑé???êÊ??ÁÁ?ÊÇ??Ô- ÉúÔ?Ö?ʵ?ʺ?Á??ÆËã??,?ÓÈë8??Á?Ë?,?þÅÝ30 min???åÖó1 h???ýÂË??ÖØ???åÖó3?Î??ºÏ??ÂËÒº??Å?ËõΪ1?Ã2????Ã?ºÁÉýÒ?ÒºÖÐÏàµ?ÓÚº?Ô-ÉúÒ?2 g??Ò?Òº???ßÎÂÃð?ú,ÖÃ?ùÏäÄÚ??ʵÑé??ÓÃ?? ????1.1.5 Ô?Ö?×ÜÔíÜÕ?Î??Ïà?ØÎÄÏ×?Û1?Ý,??ÉúÔ?Ö??Ö?Û300 g?-Ê?ÓÍÃÑÍÑÖ?ºó?éÂË??Ò?Ôü?Ó?É??ÓÃ95%µÄÒÒ???ØÁ?Ìá?ö???ÃÈÈ?éÂË??ÖØ??4?Î??ºÏ??ÂËÒº????Å?ËõÖÁÔ?300 ml???ÓÈë3??Á?ÒÒÃÑ?Áµí???ÅÖÃ?ýÒ???ÇãÈ?ÉÏÇåÒº?????Áµí?õÑ??ÉÔï???ÆÖØ??º?Á?Ϊ2.21%?? ????1.2 ??ÎïÆÕÍ????µ?ÒÍÃ??ÌåÖØ2~3 kg,ÓÉ?É??ÖÐÒ?Ò??óÑ?ʵÑé??ÎïÖÐÐÄÌá????ºÏ?ñÖ?ºÅ:SCXK(??)2004?14?? ????1.3 Ò?Æ?1?Á10?- 5 g/mlÂÈ??ÒÒõ?µ??îÈÜÒº??ÉϺ???Ñ?ÊÔ?Á×Ü??ËùÊôÉϺ?ÊÔ?ÁÈý??????10??ÂÈ???µÈÜÒº???É????Ñ?ÊÔ?Á??????1?Á10?- 5g/mlÁ×Ëá×é??ÈÜÒº??SIGMA??Ë???ÅúºÅ16H0653???? ????1.4 ÊÔ?ÁÌ?ÊÏÒº??ÅäÖÃ?????Î??Ïà?ØÎÄÏ×?Û2?Ý?? ????1.5 Æ??ÄHW-400SºãÎÂÆ??????Û???É??Ì?ÃË?Æ??ÓÐÏÞ??Ë?????BL- 420ÉúÎï?úÄÜʵÑéϵÍ????É??Ì?ÃË?Æ??ÓÐÏÞ??Ë?????Ñ?Á???ÄÜÆ???ÊÖÊõÆ?е??1 ml?ɵ?Ê?ÒÆÒºÇ?µÈ?? ????1.6 Í??ÆÑ???ÀíËùÓÐÊý?ÝÒÀ?ÝSPSS12.0?æÍ??ÆÈí?þ?øÐÐt?ìÑé?ÖÎö??Àí?? ????2 ????Óë?á?û ????È??ÒÍÃÃÍ??Í???ÖÂËÀ??Ñ?ËÙÆÊ????È??üÓÄÃÅ?ËÊ??þÖ???Ô?10 cm??ÖÃÓÚÊ?ÓÐÀäÌ?ÊÏÒºÖ?Æ?ÃóÖÐ??ÑØ???Ú?ôÈ???ϵÄ????????Ü?ô?É1.5~2 cmµÄÐ?Î??ÓÃ??µ??ÚºãÎÂÆ??????Û??Ê?Ë?ÎÂ???ÖÔÚ??37?À0.5???æ????Ñ?Á???ÄÜ? 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