离体兔肺静脉肌袖心肌细胞电生理特性

离体兔肺静脉肌袖心肌细胞电生理特性 常规玻璃微电极:离体兔肺静脉肌袖心肌细胞电生理特 性 【中文摘要】:本实验应用常规玻璃微电极细胞内记录技术,研究 家兔肺静脉肌袖(Pulmonary vein cadiomyocytes, PVC)电生理特性, 观察肺静脉肌袖心肌细胞与心房肌细胞之间的电传导现象,探讨肺静 脉起源的阵发性房颤的发生机制。方法: 50只健康成年家兔,获取肺 静脉及相连左房心肌组织,应用常规微电极细胞内技术:1、记录PVC 与左心房心肌细胞(Atrial cardiomyocytes, LAC)动作电位(Action potential, AP),并进行各项参数比较(APD20,APD50,APD90);2、分别 于PVC端和LAC端予不同频率脉冲刺激,观察另一端的动作电位的产 生情况;3、改用加有钾离子通道阻滞剂2mmol/L氯化铯(Caesium chloride,CsCl)的台式液,观察二者早后除极发生的可能性。结果:1、 PVC的APD20,APD50,APD90均较LAC长(APD20 23.09?5.44vs19.5? 1.658,APD50 52.4?7.93vs28.9?4.363,APD90 123.35? 8.26vs69.65?9.44),有显著统计学差异(n=10,P<0... 【英文摘要】: Applying intracellular recording mode of the standard microelectrode technique to study the electrophysiological characteristics of cardiomyocytes from rabbit pulmonary vein sleeves (PVC), and observe the electrical conduction between PVC and left atrial cardiomyocytes(LAC). To explore the mechanism of paroxysmal atrial fibrillation originated from the pulmonary vein (PV).Methods: Multiple cardiomyocyte preparations from PV and LA of 50 healthy adult rabbits were obtained. The standard intrac... 【关键词】常规玻璃微电极 肺静脉肌袖 左心房 心肌细胞 动作 电位 早后除极 【英文关键词】Standard microelectrode technique Pulmonary vein sleeves Left atrial cardiomyocytes Early after depolarization Action potential 【目录】离体兔肺静脉肌袖心肌细胞电生理特性 中文摘要 4-5 Abstract 5 中英文缩写对照表 7-8 引言 8-12 材料与试剂 12-17 实验方法 17-20 实验结 果 20-26 讨论 26-29 结论 29-30 参考文献 30-33 致谢 33-34 综述 34-38 参考文献 37-38