【doc】细胞角蛋白19与胰腺干细胞
细胞角蛋白19与胰腺干细胞
Review
中国组织工程研究与临床康复第11卷第11期2007-03-18出版
JournalofClinicalRehabilitativeTissueEngineenngResearchMarch18,20
07Vo1.11,No.11
Cytokeratin19andpancreasstemcells?
YangChuang,WangJi—ming,DuChengyou
‘DepartmentofLiver
andGallSurgery.the
ThirdPeople’s
HospitalofMianyang,
Mianyang612O0o.
SichuanProvince,
China;partmentof
LivetandGalJ
Su~ew,theFirst
AffiliatedHospitalof
ChongqingMedical
University,Chonoq~j
400016.China
YangChuang”Jr,
MastaAffending
phys;dan,Department
ofLiverandGalJ
Surgery,theThird
People’sHospnalof
Mianyang.Mianyang
612000.Sichuan
Province,China
chuang1975@
126.com
Received:2(Y)6-06-22
Accepted:2006-10-17
(06-50-6-5047/G)
YangC,WangJM,
DuCY.Cytokeratin19
andpancreasstem
cells.ZhongguoZuzhi
GongchengYanjiuyu
LinchuangKangfu
2007:11(11):
2194-2196(China)
Mw.g.om,
zglckf/eiournal/
upfiles/07-11/
11k-2194(ps).pdfl
2194
Abstract
BACKGROUND:Tosyntheticallyanalyzethecorrelationbetweencytokeratin19andpancreasstemcells
DATASOURCES:Referencesaboutthecorrelationbetweencytokeratin19andpancreasstemcellswereretrievedin
MedlineandOviddatabasewiththekeywordsof”cytokeratin.pancreas.stemcells.marker.differentiatio
.
n”inEnglish
fromJanuary2000toMay2006.
STUDYSELECTION:Literaturesaboutthecorrelationbetweencytokeratin19andpancreasstemcellswereretrieved
andthosecharacterizedbystrongpertinency.publishedinthenearfutureandselected仃omauthoritativejournalswere
selectedinthispaper.
DATAEXTRACTION:Among86articles,35ofthemmettheinclusioncriteriaand51wereexcluded
DATASYNTHESIS:Pancreasstemceilsarecharacterizedbymulti—directi
onaldifferentiation.Cytokeratin19hasa
positiveexpressioninpancreasstemcellsandamolecularsignofpancreasstemcells.Thisprovidesanewwayto
furtherunderstandbionomicsofpancreasstemcells,studyseparation.purification.transconformationanddirection
differentiationtoisletcells.andtreatdiabetesmellituswithtransplantationofpancreasstemcells.
CONCLUSION:Expressionofcytokeratin19isobservedduringthedifferentiationofpancreasstemcellsandcytokeratin
19isasignofpancreasstemcells.
INTRoDUCTIoN
Existingresearcheshaveprovedtheexistenceof
pancreasstemcells.whicharecharacterizedby
multi-directionpotentialdifferentiationandcandjf『erentiate
jntoendocrinous_functioncellsandexacrinous—function
cells”.Cytokeratin19isatypicalmarkerofendotheliaI
cell;while.itspositiveexpressionjsobservedInpancreas
stemcells.whicharecharacterizedby
transconformation-dif『erentiationpotencythrough
detectingpositiveexpressionofcytokeratin19.Inaddition.
cytokeratin19isregardedasamarkerofpancreasstem
cells~’-】.Thispaperisdesignedtoanalyzethecorrelation
betweencytokeratin19andpancreasstemcells.
21)jsonekindsofproteintoconsistof Cytokeratin(CK1—
cytoskeletonandakindofdifferentiatedspecificprotein.
Basedon{soionicpoint.jtisclass币edjntoacidictype-I
protein(K9—20)andbasictype—llprotein(K1—8).
Cytokeratin19isafragmentofcytokeratin.belongsto
acidicprotein.andakindofjnterzonaIfiberjnendotheliaI
celIskeletonF.S1.Therelativemolecularmass(RMM)0f
cytokeratin19is4000.andjtconsistsofabout300kinds
ofaminoacid.Especially.itshighconservativea—helicaI
structurejncentralareaisthebasicstvIeofsilk-like
structure.Ascomparedwithotheracidicproteins.
althoughcytokeratin19isIackofC.endinnon.helicaI
tailedarea,jtjsabletocompletelymatchtobasicproteins
withsilk—likestructure.However.thecompoundjsnot
stable.Humancytokeratin19isJocatedatchromosome
17q-22,andgeneIOCUSofcytokeratin19ofratsjshighly
homologousserieswithtelatiregeneIocusofhuman
cytokeratin19.whichjsJocatedatthe11chromosomeof
basicproteinfamily.
Cytokeratin19hasahighexpressioninvarioustissue
precursorcells.Ontheviewpointofdifferentiation,
cytokeratin19expressesasaparticularendotheliaIcelI
andismainlyIocatedassimpleendotheliaIcells.suchas
tracheaIepithelium.entericepitheliumandpancreaticduct
epithelium.AIotofresearchesdemonstratethat.highly
positiveexpressionjsabundantintissuehavingabnormal
development.suchaspavement-epitheliumcancer.
adenoidcysticcarcinoma,breastcancerandpancreas
cancer.Therefore.jtactsasamarkeroftumorandjs
widelyusedforclinicaIdefection11】.However.during
recentyears.someresearchesjndicatethat[121.duringthe
developmentofpancreas.highexpressionofcytokeratin
19isobservedincatheter-likeprecursorcelIgroups.and
then.thesepositivecellsofcytokeratin19differentiatejnto
end0crjn0us—functioncellsandexacrinous—functioncells.
Thissuggeststhatpositiveofcytokeratin19isobviousin
catheterendotheliaIcells;however.afewofcytokeratin19
expressinpancreasstemcells.andtherejsno
expressionofcytokeratin19jnacinouscells.
Cytokeratin19isamarkerofpancreasstem
cells
Cytokeratin19alwaysactsasamarkeroftumorcells.but
duringrecentyears,researchesdemonstratethatpositive
expressionofcytokeratin19isobservedcnpancreasstem
cells;therefore.cytokeratin19jsamolecularmarkerof
pancreasstemcells.Duringthedevelopmentofpancreas,
endocrinous-functioncellsarederivedfrompositive
endotheliaJcellsjnendobalstofkeratin;while.
endocrinous-functioncellsshowahighexpressionof
keratinjnpancreas.BouwensIn3ipointsthat.duringthe
P.O.Box1200.Shenyang110004kf2338,~.cornw~gldd.com
杨闯,等.细胞角蛋白19与胰腺千细胞
ISSN1673-8225CN21-1539
帅0册膏置嬲垃0鲫
Nestin,ckeratin,neuronpr0七ein3(ngn?3),vimentin,sun,iVin
(bcI-2),8Pr0babIy,alot0fman’ershaVenotbeenfoundoutupt0
nOw.InvestigatingCe?-suCemarker0fpancreasstemCe?sand
ent忏yingprecisesourCeandspecmcmoIecuIarmarkeraimt0
furtherunderstandbiOnOmicsOfpancreasstemCe?s.Itis
sian币canCefOrseparatiOn,puricatiOn,trans?nfOrmatiOnand
din?ctiOndifFenjntiatiOnintOisIetce?s:meanwh.1e,transpIantatiOnOf
pancreasstemce?spr0videsahOpeforthetreatment0fdiabetes
mel?tus.
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细胞角蛋白19与胰腺干细胞?
杨闯’,王济明,杜成友
‘绵阳市第三人民医院肝胆外科,四川省绵阳市612000:重庆医科大
学附属第一医院肝胆外科.重庆市400016
杨闯?,男.1973年生,重庆市人,汉族,2006年重庆医科大学毕业,
硕士.主治医师,主要从事器官移植,肝胆外科疾病方面的研究.
摘要
目的:综合分析细胞角蛋白19与胰腺干细胞的关系.
资料来源:应用计算机检索Med?ne及0vid2000—01,2006—05有关细
胞角蛋白19与胰腺干细胞关系的文献,检索词”Cytokeratin.
Pancreas,SfemceIIs,Marker,Di忏erentiation”并限定文章语言种类为
EngIish.
资料选择:对检索到的细胞角蛋白19与胰腺干细胞关系的相关信息进
行整理,选取针对性强的文章,同一领域的文献则选择近期发表或权威
杂志的文章.
资料提炼:共检索到86篇相关文献,其中35篇文章符合
,排除51
篇.
资料综合:胰腺干细胞具有多向分化功能,细胞角蛋白19在胰腺干细胞
中有阳性表达,是胰腺干细胞的分子标志物,这对进一步了解胰腺干
细
胞的生物学特性,对胰腺干细胞的分离,纯化及转型定向分化为胰岛细
胞具有重要意义.胰腺干细胞的移植为糖尿病的治疗开辟了道路.
结论:胰腺干细胞在分化过程中有细胞角蛋白19表达.细胞角蛋白19
是胰腺干细胞的标志物.
关键词:角蛋白;干细胞;胰腺,细胞学
中图分类号:F94.2文献标识码:A文章编号:1673.8225(2OO7)11_02194_03
杨闯,王济明,杜成友.细胞角蛋白19与胰腺千细胞【J】.中国组织工程研究与临床
康复,2007,11(11):2194-2196
I,II,ww.zgIcI(f.?m,zgIckr/ejoumaI/u呻Ies/O7-11/11k-2194(ps).pd叼
(EditedbyGaoHJ,JiH,WangL)
P.O.B0x1200,Sh鲫yang110004kf23385I娥@?ina.oomwwIdoom