【doc】细胞角蛋白19与胰腺干细胞

【doc】细胞角蛋白19与胰腺干细胞 细胞角蛋白19与胰腺干细胞 Review 中国组织工程研究与临床康复第11卷第11期2007-03-18出版 JournalofClinicalRehabilitativeTissueEngineenngResearchMarch18,20 07Vo1.11,No.11 Cytokeratin19andpancreasstemcells? YangChuang,WangJi—ming,DuChengyou ‘DepartmentofLiver andGallSurgery.the ThirdPeople’s HospitalofMianyang, Mianyang612O0o. SichuanProvince, China;partmentof LivetandGalJ Su~ew,theFirst AffiliatedHospitalof ChongqingMedical University,Chonoq~j 400016.China YangChuang”Jr, MastaAffending phys;dan,Department ofLiverandGalJ Surgery,theThird People’sHospnalof Mianyang.Mianyang 612000.Sichuan Province,China chuang1975@ 126.com Received:2(Y)6-06-22 Accepted:2006-10-17 (06-50-6-5047/G) YangC,WangJM, DuCY.Cytokeratin19 andpancreasstem cells.ZhongguoZuzhi GongchengYanjiuyu LinchuangKangfu 2007:11(11): 2194-2196(China) Mw.g.om, zglckf/eiournal/ upfiles/07-11/ 11k-2194(ps).pdfl 2194 Abstract BACKGROUND:Tosyntheticallyanalyzethecorrelationbetweencytokeratin19andpancreasstemcells DATASOURCES:Referencesaboutthecorrelationbetweencytokeratin19andpancreasstemcellswereretrievedin MedlineandOviddatabasewiththekeywordsof”cytokeratin.pancreas.stemcells.marker.differentiatio . n”inEnglish fromJanuary2000toMay2006. STUDYSELECTION:Literaturesaboutthecorrelationbetweencytokeratin19andpancreasstemcellswereretrieved andthosecharacterizedbystrongpertinency.publishedinthenearfutureandselected仃omauthoritativejournalswere selectedinthispaper. DATAEXTRACTION:Among86articles,35ofthemmettheinclusioncriteriaand51wereexcluded DATASYNTHESIS:Pancreasstemceilsarecharacterizedbymulti—directi onaldifferentiation.Cytokeratin19hasa positiveexpressioninpancreasstemcellsandamolecularsignofpancreasstemcells.Thisprovidesanewwayto furtherunderstandbionomicsofpancreasstemcells,studyseparation.purification.transconformationanddirection differentiationtoisletcells.andtreatdiabetesmellituswithtransplantationofpancreasstemcells. CONCLUSION:Expressionofcytokeratin19isobservedduringthedifferentiationofpancreasstemcellsandcytokeratin 19isasignofpancreasstemcells. INTRoDUCTIoN Existingresearcheshaveprovedtheexistenceof pancreasstemcells.whicharecharacterizedby multi-directionpotentialdifferentiationandcandjf『erentiate jntoendocrinous_functioncellsandexacrinous—function cells”.Cytokeratin19isatypicalmarkerofendotheliaI cell;while.itspositiveexpressionjsobservedInpancreas stemcells.whicharecharacterizedby transconformation-dif『erentiationpotencythrough detectingpositiveexpressionofcytokeratin19.Inaddition. cytokeratin19isregardedasamarkerofpancreasstem cells~’-】.Thispaperisdesignedtoanalyzethecorrelation betweencytokeratin19andpancreasstemcells. 21)jsonekindsofproteintoconsistof Cytokeratin(CK1— cytoskeletonandakindofdifferentiatedspecificprotein. Basedon{soionicpoint.jtisclass币edjntoacidictype-I protein(K9—20)andbasictype—llprotein(K1—8). Cytokeratin19isafragmentofcytokeratin.belongsto acidicprotein.andakindofjnterzonaIfiberjnendotheliaI celIskeletonF.S1.Therelativemolecularmass(RMM)0f cytokeratin19is4000.andjtconsistsofabout300kinds ofaminoacid.Especially.itshighconservativea—helicaI structurejncentralareaisthebasicstvIeofsilk-like structure.Ascomparedwithotheracidicproteins. althoughcytokeratin19isIackofC.endinnon.helicaI tailedarea,jtjsabletocompletelymatchtobasicproteins withsilk—likestructure.However.thecompoundjsnot stable.Humancytokeratin19isJocatedatchromosome 17q-22,andgeneIOCUSofcytokeratin19ofratsjshighly homologousserieswithtelatiregeneIocusofhuman cytokeratin19.whichjsJocatedatthe11chromosomeof basicproteinfamily. Cytokeratin19hasahighexpressioninvarioustissue precursorcells.Ontheviewpointofdifferentiation, cytokeratin19expressesasaparticularendotheliaIcelI andismainlyIocatedassimpleendotheliaIcells.suchas tracheaIepithelium.entericepitheliumandpancreaticduct epithelium.AIotofresearchesdemonstratethat.highly positiveexpressionjsabundantintissuehavingabnormal development.suchaspavement-epitheliumcancer. adenoidcysticcarcinoma,breastcancerandpancreas cancer.Therefore.jtactsasamarkeroftumorandjs widelyusedforclinicaIdefection11】.However.during recentyears.someresearchesjndicatethat[121.duringthe developmentofpancreas.highexpressionofcytokeratin 19isobservedincatheter-likeprecursorcelIgroups.and then.thesepositivecellsofcytokeratin19differentiatejnto end0crjn0us—functioncellsandexacrinous—functioncells. Thissuggeststhatpositiveofcytokeratin19isobviousin catheterendotheliaIcells;however.afewofcytokeratin19 expressinpancreasstemcells.andtherejsno expressionofcytokeratin19jnacinouscells. Cytokeratin19isamarkerofpancreasstem cells Cytokeratin19alwaysactsasamarkeroftumorcells.but duringrecentyears,researchesdemonstratethatpositive expressionofcytokeratin19isobservedcnpancreasstem cells;therefore.cytokeratin19jsamolecularmarkerof pancreasstemcells.Duringthedevelopmentofpancreas, endocrinous-functioncellsarederivedfrompositive endotheliaJcellsjnendobalstofkeratin;while. endocrinous-functioncellsshowahighexpressionof keratinjnpancreas.BouwensIn3ipointsthat.duringthe P.O.Box1200.Shenyang110004kf2338,~.cornw~gldd.com 杨闯,等.细胞角蛋白19与胰腺千细胞 ISSN1673-8225CN21-1539 帅0册膏置嬲垃0鲫 Nestin,ckeratin,neuronpr0七ein3(ngn?3),vimentin,sun,iVin (bcI-2),8Pr0babIy,alot0fman’ershaVenotbeenfoundoutupt0 nOw.InvestigatingCe?-suCemarker0fpancreasstemCe?sand ent忏yingprecisesourCeandspecmcmoIecuIarmarkeraimt0 furtherunderstandbiOnOmicsOfpancreasstemCe?s.Itis sian币canCefOrseparatiOn,puricatiOn,trans?nfOrmatiOnand din?ctiOndifFenjntiatiOnintOisIetce?s:meanwh.1e,transpIantatiOnOf pancreasstemce?spr0videsahOpeforthetreatment0fdiabetes mel?tus. 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Pancreas,SfemceIIs,Marker,Di忏erentiation”并限定文章语言种类为 EngIish. 资料选择:对检索到的细胞角蛋白19与胰腺干细胞关系的相关信息进 行整理,选取针对性强的文章,同一领域的文献则选择近期发或权威 杂志的文章. 资料提炼:共检索到86篇相关文献,其中35篇文章符合要求,排除51 篇. 资料综合:胰腺干细胞具有多向分化功能,细胞角蛋白19在胰腺干细胞 中有阳性表达,是胰腺干细胞的分子标志物,这对进一步了解胰腺干 细 胞的生物学特性,对胰腺干细胞的分离,纯化及转型定向分化为胰岛细 胞具有重要意义.胰腺干细胞的移植为糖尿病的治疗开辟了道路. 结论:胰腺干细胞在分化过程中有细胞角蛋白19表达.细胞角蛋白19 是胰腺干细胞的标志物. 关键词:角蛋白;干细胞;胰腺,细胞学 中图分类号:F94.2文献标识码:A文章编号:1673.8225(2OO7)11_02194_03 杨闯,王济明,杜成友.细胞角蛋白19与胰腺千细胞【J】.中国组织工程研究与临床 康复,2007,11(11):2194-2196 I,II,ww.zgIcI(f.?m,zgIckr/ejoumaI/u呻Ies/O7-11/11k-2194(ps).pd叼 (EditedbyGaoHJ,JiH,WangL) P.O.B0x1200,Sh鲫yang110004kf23385I娥@?ina.oomwwIdoom