姜黄生姜醇提物对幼龄SD大鼠前列腺的作用

姜黄生姜醇提物对幼龄SD大鼠前列腺的作用 【摘要】  目的观察姜黄与生姜醇提物对幼龄SD大鼠前列腺生长的影响。方法 取姜黄与生姜各500 g，粉碎混匀后加70%乙醇回流提取制得醇提物;取体质量约50 g的SD大鼠随机分成正常对照组、阳性药物组、醇提物低、中、高剂量组，每组13只。正常对照组及阳性药物组均按20 mL·kg-1体质量灌胃(ig)给予生理盐水(NS)，另阳性药物组按1 mg·kg-1体质量皮下注射(sc)己雌酚，每周3次;低、中、高剂量组按20 mL·kg-1体质量ig给予醇提物，连续3周。3周后，测定血清总酸性磷酸酶(TAP)水平，再取睾丸、精囊腺、前列腺(腹叶、侧叶、背叶)分别称取湿重，计算各指数。结果 与正常对照组比较，醇提物中、高剂量组的大鼠前列腺生长均受到明显抑制(P<0.05)，低剂量组虽显示一定的抑制作用，但无统计学意义;醇提物低、中、高剂量对大鼠睾丸生长影响不大而对精囊腺影响显著(P<0.01);醇提物低、中、高剂量均能显著降低TAP的水平(P<0.05或P<0.01)。结论 姜黄生姜醇提物中、高剂量均明显抑制幼龄大鼠的前列腺生长，对睾丸的生长无明显影响。 【关键词】  姜黄;生姜;幼龄大鼠;良性前列腺增生(BPH) 　Abstract:Objective To observe the combined effects of Curcuma and Ginger on the growth of prostate of juvenile Sprague Dawley rats. Methods Take Curcuma and Ginger 500 g respectively,add 70% ethanol to get the ethanol extracts. SD rats with body weight about 50 g were randomly divided into normal control group,positive medicine group,ethanol extracts of low-,mid- and high-dose groups,with 13 rats in each group. Rats in the normal group and positive medicine group were given NS at 20 mL per kg body weight,and rats in the positive medicine group were treated by diethylstilbestrol through subcutaneous injection at 1 mg per kg body weight three times a week. Rats in low-,mid- and high-dose groups were treated by ethanol extract through intragastric administration at 20 mL per kg body weight. Three weeks later,the level of total acid phosphatase(TAP)in serum was determined and the testis,seminal vesicle and prostate (ventral,lateral and dorsal)were separated and weighted for viscera index. Results In comparison with the normal control group,significant inhibition was observed in prostate growth in mid- and high-dose groups(P<0?05)in three weeks,while the similar effect was observed in the rats in low dose group but with no statistical significance. No side effect on testis was observed in the rats in various groups,while significant effect on seminal vesicle was observed. Serological tests of TAP showed that TAP levels of the rats in the low-,mid-,and high-dose groups decreased significantly(P<0.05 or 0.01). Conclusion The growth of prostate of juenile Sprague Dawley rats in mid- and high-dose groups were inhibited significantly,and no side effect on testis was observed. 　　Key words:Curcuma;Ginger;juvenile rats;benign prostatic hyperplasia(BPH) 　　良性前列腺增生(benign prostatic hyperplasia，BPH)是困扰老年男性的常见疾病，以排尿困难、尿闭等为主要临床特征。姜黄根茎中主要含挥发油类及姜黄素两大类成分。姜黄素类为二苯基庚烃类成分，主要含姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素[1]。生姜的块茎主要含挥发油类、姜辣素、二苯基庚烷[2]。杨磊等[3]的研究表明姜黄素能抑制雄激素受体的表达，说明姜黄素具有类雌激素的作用。Shukla等[4]通过研究发现[6]-姜酚(姜辣素)在LNCap细胞和大鼠前列腺腹叶中对睾酮诱导的抗凋亡蛋白bcl-2和存活素的表达具有向下调节作用，表明[6]-姜酚可能具有一定的抗雄性激素作用。可见，两者可能具有的类雌激素作用与治疗前列腺增生的抑制雄性激素的水平相符。当前市面上用于治疗前列腺增生的制剂中未见有姜黄或生姜。本研究以姜黄为主辅以生姜，考察两者组合的醇提物对幼龄SD大鼠前列腺的影响，为开拓良性前列腺增生的用药范围提供参考。现报道如下。 　　1材料 　　1.1药物 　　姜黄(批号080401D510，四川)与生姜(购自农贸市场)经广州中医药大学青蒿研究中心朱宇同教授鉴定，分别为姜科植物姜黄Curcuma longa L.的干燥根茎与姜科植物姜Zingiber officinale Rosc.的新鲜根茎;姜黄素对照品(自制)，经HPLC法测定纯度为98.6%;己雌酚注射液(2 mg/mL，批号080605，上海通用药业股份有限公司)。 　　1.2动物 　　SD大鼠，雄性，体质量45～55 g，SPF级，由广东省医学实验动物中心提供。实验动物质量合格证号0046490，许可证号：SCXK(粤)2008-0002，粤监证字2008A020。 　　1.3仪器 　　LC-10A高效液相色谱仪，SPD-M10Avp光二极管阵列器(日本岛津公司);Olympus全自动生化分析仪(AU5421，日本奥林巴斯公司)，由南方医院检验科提供;Startorius(BS224S)万分之一电子天平(德国赛多利斯公司)。 　　1.4实验方法