低剂量微波辐射对增强UV_B辐射损伤菘蓝幼苗抗氧化酶活性的影响_英文_
西北植物学报Κ2005Κ25; 11ΓΠ2219—2225
A cta B ot. B orea l. -O cciden t. S in.
文章编号Π100024025; 2005Γ1122219207
低剂量微波辐射对增强 UV-B 辐射损伤菘蓝
幼苗抗氧化酶活性的影响α
陈怡平1Κ2Κ刘永军3Κ赵萌萌4Κ崔 瑛2; 1 中国科学院地球环境研究所Κ西安 710075Μ2 西安工程科技学院 生物工程系Κ西安 710048Μ3 西安建筑科技大学 环境学院Κ西
安 710054Μ4 西北大学 生命科学学院Κ西安 7100...
西北植物学报Κ2005Κ25; 11ΓΠ2219—2225
A cta B ot. B orea l. -O cciden t. S in.
文章编号Π100024025; 2005Γ1122219207
低剂量微波辐射对增强 UV-B 辐射损伤菘蓝
幼苗抗氧化酶活性的影响α
陈怡平1Κ2Κ刘永军3Κ赵萌萌4Κ崔 瑛2; 1 中国科学院地球环境研究所Κ西安 710075Μ2 西安工程科技学院 生物工程系Κ西安 710048Μ3 西安建筑科技大学 环境学院Κ西
安 710054Μ4 西北大学 生命科学学院Κ西安 710069Γ
摘 要Π为了探讨低剂量微波对增强UV 2B 辐射损伤菘蓝; Isa tis ind ig otica Fo rt. Γ的修复作用Κ将经过 10. 08 kJ·
m
- 2·d- 1辐射损伤; PA R = 220 Λmo l·m - 2·s- 1Γ的菘蓝幼苗分别经 0、3、6、9 和 12 s 等不同时间的微波辐照; 126
mW ·cm - 2Κ2 450 M H zΓΚ然后测定其幼苗M DA 含量、紫外吸收物质含量、抗坏血酸含量以及 3 种抗氧化酶 SOD、
CA T 和 POD 活性Λ结果
明Κ增强UV 2B 辐射损伤菘蓝在微波的作用下其菘蓝幼苗中 SOD、CA T 和 POD 活性及
紫外吸收物质含量、抗坏血酸含量提高ΚM DA 含量得到显著的降低Κ说明微波对增强UV 2B 辐射伤害菘蓝幼苗具
有修复作用Λ但是Κ随着微波剂量的增加Κ这种修复效应减弱Κ甚至消失Λ上述参数的变化说明适量时间的微波处理
可以提高菘蓝对增强UV 2B 辐射的抵抗能力Κ并在此基础上初步探讨了微波的修复机理Λ
关键词Π菘蓝Μ微波ΜUV 2B 辐射Μ修复效应
中图分类号ΠQ 947. 9 文献标识码ΠA
Inf luence of L ow D osesM icrowave Rad ia tion on Activ it ies of
An tiox ida tive Enzym es of Isa tis ind igotica Seedl ings
Exposed to Enhanced UV-B Rad ia tion
CH EN Y i2p ing1Κ2ΚL IU Yong2jun3ΚZHAO M eng2m eng4ΚCU I Y ing2; 1 Institu te of Earth Environm entΚCh inese A cadem y of SciencesΚX ipian 710075ΚCh inaΜ2 D epartm en t of B ioengineeringΚX ipian U ni2
versity of Engineering Science and T echno logyΚX ipian 710048ΚCh inaΜ3 Co llege of Environm ent ScienceΚX ipian U niversity of A rch i2
tectu re and T echno logyΚX ipian 710054ΚCh inaΜ4 Co llege of L ife ScienceΚN o rthw est U niversityΚX ipian 710069ΚCh inaΓ
AbstractΠIn o rder to determ ine the dam age repair ro le of m icrow ave in the st ress resistance of Isa tis ind ig 2
otica to UV 2B radia t ionΚthe seedlings sub jected to UV 2B radia t ion of 10. 08 kJ·m - 2·d- 1 fo r 8 h under
pho to syn thet ica lly act ive radia t ion ; 220 Λmo l·m - 2·s- 1Γw ere expo sed to m icrow ave radia t ion of 0Κ3Κ6Κ9
and 12 s ; 126 mW ·cm - 2Κ2 450M H zΓ. A fterw ardsΚchanges of UV 2B 2in ju red leaves of the fou rth in the ac2
t ivit ies of enzym esΚsuch as SOD ΚCA T and POD Κand in the concen tra t ion of m alondia ldehyde ;M DA Γ and
asco rb ic acid ;A sA ΓΚand UV ab so rb ing compounds w ere m easu red to test the effects of m icrow ave trea t2
m en t. T he resu lts ind ica ted w hen the seedlings w ere trea ted u sing 10. 08 kJ·m - 2·d - 1 UV 2B and then ex2
po sed to sho rter m icrow ave radia t ionΚthe act ivit ies of SOD ΚCA T and POD Κand the concen tra t ion of A sA Κ
and UV ab so rb ing compounds increased sign if ican t ly. UV 2B 2in ju red Isa tis ind ig otica seedlings trea ted w ith
m icrow ave radia t ion recovered faster from enhanced UV 2B stress resistance becau se the M DA concen tra2α 收稿日期Π2005203225Μ修改稿收到日期Π2005209223
基金项目Π中国博士后科学基金资助; 2005037245ΓΜ国家自然科学基金资助; 30370269Γ
作者简介Π陈怡平; 1968- ΓΚ男;汉ΓΚ教授Κ博士Κ主要从事环境植物学的研究Λ
t ion decreased compared w ith UV 2B treatm en t a lone. How everΚthe repaired effect on seedlings in ju red by
UV 2B dam age becam e w eakΚand even lo st w ith the augm en t of m icrow ave radia t ion t im e. T ho se changes in
the vigo r of enzym es and in concen tra t ion ofM DA ΚA sA and UV 2B ab so rb ing compound suggested that m i2
crow ave radia t ion of su itab le t im e length had a po sit ive physio logica l repair ro le on UV 2B 2dam aged p lan ts.
How everΚthe m echan ism s of m icrow ave repair effect in p lan ts dam aged by UV 2B are no t clear a t p resen t
and addit ional w o rk w ill be needed to invest iga te in the fu tu re.
Key wordsΠIsa tis ind ig oticaΜm icrow aveΜUV 2B lesionΜrepair ro le
U ltravio let2B radia t ion levels are st ill rela t ive2
ly h igh at the earthpis su rface due to the dep let ion of
st ra to spheric ozone by the ch lo rofluo rocarbon s re2
leased in to the atmo sphere since the m id2n ine2
teen th cen tu ry. Cu rren t UV 2B levels are expected
to increase in the even t of M on trea l P ro toco l no t
being fu lly imp lem en ted by the m em ber coun2
t ries[1 ]. UV 2B direct ly dam ages p lan tspi cellu lar
m em b ranes and free radica l elim inat ion system s
such as severa l an t iox idat ive enzym es including su2
perox ide dism u tase ; SOD ΓΚcata lase ;CA T ΓΚperox2
idase ; POD Γ and so on. T herefo reΚUV 2B 2induced
reduct ion s in p lan t grow th o r p roduct ivity are
linked to altered leaf physio logy and funct ion [2 ]. E l2
evated UV 2B radia t ion can be con sidered a sign if i2
can t po ten t ia l environm en ta l st ress in natu ra l e2
co system s and agricu ltu re. P reviou s research has il2
lu st ra ted that h igh pho to syn thet ica lly act ive radia2
t ion ; PA R Γ[3 ]Κh igh concen tra t ion of CO 2 [4 ]Κand
low n it rogen supp ly [5 ] can each allevia te cell dam 2
age induced by UV 2B irrad ia t ion. R ecen t lyΚQ iΚet
a l. [6 ] also repo rted that laser can also allevia te cell
dam age of V icia f aba L. ;L egum ino seaΓ induced by
UV 2B irrad ia t ion.
N um erou s studies have demon stra ted that m i2
crow ave radia t ion has a po sit ive ro le of accelera t ing
p lan ts seeds germ inat ion [7 ]Κ imp roved o il quality
and increased yield of grape seed [8 ]Κand imp rove
m ale and fem ale flow er fo rm at ion of cucum ber [9 ].
To ou r know ledgeΚhow everΚlit t le is know n abou t
the dam age repair ro le of m icrow ave trea tm en t in
Isa tis ind ig otica expo sed to enhanced UV 2B radia2
t ion. In the p resen t studyΚw e have exam ined the
an t iox idat ive enzym es act ivit iesΚthe concen tra t ion
of A sA ΚUV ab so rb ing compounds and M DA con2
cen tra t ion to test the hypo thesis tha t w hether the
m icrow ave has the repair effect on the seedlings in2
ju red by enhanced UV 2B radia t ion.
1 M ateria ls and m ethods
1. 1 Plan t ma ter ia ls
Co llege of Pharm acy of X ipian J iao tong U n iver2
sity supp lied seeds of Isa tis ind ig otica as m ateria ls.
1. 2 M ethods
1. 2. 1 Seeds germ ina tion U n ifo rm and p lump
seeds w ere selected fo r experim en tΚthey w ere ster2
ilized fo r 10 m in by 0. 05% H gC l2 and w ashed fo r
30 m in by flow ing w ater. T hey w ere grow n in p las2
t ic po ts ; 30 cm ×35 cm ΓΚf lu shed daily w ith a 10
mL half2st rength so lu t ion cu ltu re m edium of M S.
Every experim en t is comp rised of 18 p last ic po tsΚ
each p last ic po t con ta ined 20. T he po ts w ith seeds
w ere incubated in grow th cham ber w ith hum idity;M odelΠ515HD ΚU SA Γ at 25℃Κ70% rela t ive hu2
m idityΚand 400 Λmo l·mo l- 1 CO 2; am b ien t level in
the bu ild ing Γ. W hen the seedlings w ere 10 d o ld
under ligh t condit ion s of a PA R of 220 Λmo l·m - 2
· s- 1Κthey w ere divided in to 6 group sΚ3 po ts per
group. O ne w as u sed as the con tro l ;w ithou t UV 2B
and m icrow ave radia t ionΚCK ΓΚo thers as fo llow Π
UV 2B radia t ion alone ; B ΓΚUV 2B radia t ion fo l2
low ed by 3 s m icrow ave radia t ion ;B 3sΓΚUV 2B ra2
dia t ion fo llow ed by 6 s m icrow ave radia t ion ;B 6sΓΚ
UV 2B radia t ion fo llow ed by 9 s m icrow ave radia2
t ion ;B 9sΓΚUV 2B radia t ion fo llow ed by 12 s m i2
crow ave radia t ion ;B 12sΓ.
1. 2. 2 UV-B trea tm en t UV 2B radia t ion w as p ro2
vided by filtered Q in b rand ;Bao ji L amp Facto ryΚ
Ch inaΓ 30 W fluo rescence sun lamp s fo llow ing the
p rocedu re ou t lined by FengΚet a l. [10 ]. T he lamp s
w ere su spended above and perpendicu lar to the
p last ic po t and filtered w ith either 0. 13 mm th ick
0222 西 北 植 物 学 报 25 卷
cellu lo se diaceta te ; t ran sm ission dow n to 290 nm Γ
fo r UV 2B irrad iance o r 0. 13 mm po lyester p last ic
f ilm s ; ab so rb ing all rad ia t ion bellow 320 nm Γ as a
con tro l. Cellu lo se diaceta te f ilters w ere p reso lar2
ized. T he desired irrad ia t ion w as ob ta ined by
changing the distance betw een the lamp s and the
p last ic po t. T he spectra l irrad iance from the lamp s
w as determ ined w ith an Op tron icsM odel 742 ;Op2
t ron ics L ab sΚ O rlandoΚ FL Κ U SA Γ spectro ra2
diom eter. T he spectra l irrad iance w as w eigh ted u s2
ing a genera lized p lan t respon se act ion spec2
t rum [11Κ12 ] ; Caldw ellΚ1971Γ and no rm alized at 300
nm to ob ta in the desired level of b io logica lly effec2
t ive UV 2B radia t ion ;UV 2BBE Γ. T he do se of UV 2B
irrad ia t ion w as 10. 08 kJ·m - 2. In addit ion to UV 2
B radia t ionΚthe visib le rad ia t ion ; PA R 400~ 700
nm Γ ; 220 Λmo l·m - 2·s- 1Γw as also supp lied.
1. 2. 3 M icrowave trea tm en t M icrow ave ;pow er
den sity 126 mW ·cm - 2Κ2 450 M H zΓ direct ly irra2
dia ted the seedlings of Isa tis ind ig otica fo r 3Κ6Κ9
and 12 sΚrespect ively. Every po t w as trea ted on ly
once under m icrow ave irrad ia t ion. T he m icrow ave
oven ; Com b i2Grill m icrow ave oven W D 700Γ w as
m ade by Shunde m icrow ave in st itu te.
1. 2. 4 D eterm ina tion s M alondia ldehyde ;M DA Γ
concen tra t ion w as m easu red acco rd ing to P redieriΚ
et a l [12 ]. Samp le of the fou rth leaves ; 0. 2 g fresh
w eigh tΚFW Γ w ere taken from Isa tis ind ig otica
seedlings of 25 d and imm edia tely frozen at - 70℃
and m ateria ls w ere homogen ized in 5 mL pho sphate
buffer ;pH 6. 7ΓΚand then cen trifuged fo r 15 m in at
8 000 g. A 0. 5 mL supernatan t w as com b ined w ith
an equal vo lum e of th iobarb itu ric acid ; TBA Γ
reagen t [ 5% TBA ;W öV Γ in 20% trich lo roacet ic
acid ;W öV Γ] and bo iled at 100℃ fo r 20 m in. A b2
so rbance w as determ ined at 532 and 600 nm. M DA
concen tra t ion w as exp ressed as nmo l·m g- 1 p ro2
tein.
Concen tra t ion of A sA w as m easu red acco rd ing
to Tonam u ra [13 ]. Samp les of the fou rth leave of 25
d seedlings ; 0. 2 g FW Γw ere taken and p laced in a
mo rtar fo llow ing the addit ion of silicaΚN aC l and
10% m etapho spho ric acid. A fter tho rough grind2
ingΚthe samp le w as cen trifuged fo r 3 m in at 6 500
g. A supernatan t samp le w as taken and fixed to 25
mL Κand then a 0. 5 mL removed from the 25 mL Κ
and in p roper o rderΚ1 mL of cit ric acid2pho spho ric
acid buffer ;pH 2. 3Γand 1 mL of 2Κ62dich lo rophe2
no l indopheno l ; 30 m g·L - 1 Γ added. A fter 30 sΚ
the velocity of ab so rbance w as determ ined at 524
nm. A sA concen tra t ion w as exp ressed as Λg·g - 1
FW.
F resh samp le of 0. 5 g w ere taken from the
fou rth leaves and ex tracted in 10 mL acid if ied
m ethano l ;m ethano l2w ater2hydroch lo ric acidΚ7∶20
∶1ΚV öV Γ fo r UV 2B ab so rb ing compounds acco rd2
ing to the p rocedu re of M ireck i and T eram u ra [14 ].
T he hydroch lo ric acid w as 36% HC l. Ex tract ab2
so rbance w as m easu red w ith a spectropho tom eter; UV 22100ΜSh im adzuΚCo lum b iaΚM D ΚU SA Γ and
the ab so rbance w as arb it rarily u sed fo r analysis.
Ex tract ion of enzym es w as carried ou t a t 4℃.
5 g fresh t issues of the fou rth leaves of 25 d
seedlings homogen ized in 10 mL 0. 05 mo l·L - 1
pho sphate buffer ; pH 6. 7ΓΚafter tho rough grind2
ingΚthe samp le at 0℃ w as cen trifuged fo r 10 m in
at 10 000 g. T he supernatan t samp le w as sto red at
- 20 ℃ fo r assay. A ct ivit ies of SOD ΚPOD and
CA T w ere determ ined acco rd ing to Giannopo lit is
and R eies[15 ]ΚN akano and A sada [16 ] and Cakm akΚet
a l. [17 ]Κrespect ively. T he act ivity of enzym es w as
exp ressed as U ·m g- 1 p ro tein.
1. 2. 5 Sta tist ica l ana lysis Samp les w ere ar2
ranged in comp letely random ized design s w ith 6 in2
dependen t experim en ts w ith 6 rep lica t ion s. A ll the
values are tested 6 t im es ; n= 6Γ. Sign if ican t d iffer2
ence ; P < 0. 05 Γ w as determ ined acco rd ing to
D uncanpis m u lt ip le range tests.
2 R esu lts
Compare w ith the con tro l ;CKΓΚUV 2B radia2
t ion alone cau se a sign if ican t increase of 2. 03 nmo l
·m g- 1 p ro tein ; P < 0. 05Γin the concen tra t ion of
M DA ; F ig. 1Γ. In con trastΚw hen UV 2B irrad ia t ion
fo llow ed m icrow ave radia t ionΚM DA concen tra t ion
w as decreased in som e degree. Fo r examp leΚcom 2
pare w ith UV 2B radia t ion alone ;B ΓΚM DA concen2
122211 期 陈怡平Κ等Π低剂量微波辐射对增强UV 2B 辐射损伤菘蓝幼苗抗氧化酶活性的影响;英Γ
t ra t ion of UV 2B irrad ia t ion fo llow ed 6 s m icrow ave
radia t ion w as the least among fou r t rea tm en tsΚit
w as 1. 93 nmo l·m g - 1 p ro tein; P < 0. 05Γ less than
that of B group ΜUV 2B irrad ia t ion fo llow ed 3Κ9 and
12 s m icrow ave radia t ionΚ the concen tra t ion of
M DA w as 1. 53 nmo l·m g- 1 p ro tein ; P < 0. 05ΓΚ
1. 70 nmo l·m g- 1 p ro tein ; P < 0. 05ΓΚ0. 13 nmo l·
m g - 1 p ro tein ; P < 0. 05Γ less than that of B group Κ
respect ively.
F ig. 1 M DA conten ts of the seedlings in ju red by en2
hanced UV 2B radiat ion in treatm ents of m icrow ave ra2
diat ion
T here are six treatm en tsΠ; 1Γ the con tro l ; CK ΓΜ; 2Γ UV 2B
treatm en t;BΓΜ; 3ΓUV 2B treatm en t fo llow ed by 3 s m icrow ave
treatm en t ; B3sΓΜ; 4 ΓUV 2B treatm en t fo llow ed by 6 s m i2
crow ave treatm en t;B6sΓΜ; 5ΓUV 2B treatm en t fo llow ed by 9 s
m icrow ave treatm en t;B9sΓΜ; 6ΓUV 2B treatm en t fo llow ed by
12 s m icrow ave treatm en t ;B12sΓ ; T he no tes are the sam e as
below Γ
F ig. 2 A sA conten ts of the seedlings in ju red by enhanced
UV 2B radiat ion in the treatm ents of m icrow ave radia2
t ion
F ig. 3 A bso rbance at 300 nm of the seedlings in ju red by
enhanced UV 2B radiat ion
Sim ilarlyΚF ig. 2 show n that UV 2B radia t ion
alone cau se a sign if ican t decrease of 31. 3 Λg·g - 1
FW ; P < 0. 05Γ in the concen tra t ion of A sA com 2
pare w ith the con tro l ;CKΓ. In con trastΚw hen UV 2
B irrad ia t ion fo llow ed m icrow ave radia t ionΚconcen2
t ra t ion of A sA w as differen t degree increased. Fo r
examp leΚUV 2B irrad ia t ion fo llow ed 3Κ6Κ9 and 12 s
m icrow ave radia t ionΚand the concen tra t ion of A sA
w as increase of 28. 8 Λg·g- 1 FW ; P < 0. 05ΓΚ57. 6Λg·g- 1 FW ; P < 0. 05ΓΚ23. 6 Λg·g - 1 FW ; P <
0. 05ΓΚ12. 2 Λg·g- 1 FW ; P < 0. 05Γcompared w ith
UV 2B treatm en t a loneΚrespect ively.
F ig. 4 SOD activit ies of the seedlings in ju red by en2
hanced UV 2B radiat ion in the treatm ents of m icrow ave
radiat ion
Compare w ith the con tro l ;CKΓΚUV 2B radia2
t ion alone cau se a sign if ican t increase of 0. 027% ; P
< 0. 05Γin the concen tra t ion of UV 2B ab so rb ing
compounds ; F ig. 3Γ. W hen UV 2B irrad ia t ion fo l2
2222 西 北 植 物 学 报 25 卷
low ed m icrow ave radia t ionΚab so rb ing compounds
w as increased drast ica lly. Fo r examp leΚcompare
w ith B group ;B ΓΚUV 2B irrad ia t ion fo llow ed 3Κ6Κ9
and 12 s m icrow ave radia t ionΚand concen tra t ion of
UV 2B ab so rb ing compounds w as 0. 158% ; P < 0.
05ΓΚ0. 312% ; P < 0. 01ΓΚ0. 24% ; P < 0. 05ΓΚ0. 19%; P < 0. 05Γmo re than that of B group Κrespect ively.
F ig. 5 CA T activit ies of the seedlings in ju red by en2
hanced UV 2B radiat ion in the treatm ents of m icrow ave
radiat ion
A ctivit ies of SOD and CA T w ere declined
rap id ly by UV 2B radia t ion compared w ith con tro l; F ig. 4 and F ig. 5ΓΚw h ich w ere 1. 5 U ·m g- 1 p ro2
tein ; P < 0. 05ΓΚ4. 79 U ·m g- 1 p ro tein; P < 0. 05ΓΚ
low er than that of CKΚrespect ively. O n the con2
t raryΚw hen UV 2B treated seedlings w ere expo sed
to 3 s m icrow ave radia t ionΚact ivit ies of SOD and
CA T w ere 1. 1 U ·m g- 1 p ro tein ; P < 0. 05ΓΚ16. 09
U ·m g - 1 p ro tein ; P < 0. 05Γ h igher than that of
UV 2B radia t ion aloneΜexpo sed to 6 s m icrow ave ra2
dia t ionΚact ivit ies of SOD and CA T w ere 1. 76
U ·m g- 1 p ro tein ; P < 0. 05ΓΚ17. 54 U ·m g- 1 p ro2
tein ; P < 0. 01Γh igher than that of UV 2B radia t ion
aloneΜ9 s m icrow ave radia t ion of UV 2B 2induced
seedlings cau se a sign if ican t imp rovem en t of 1. 95
U ·m g- 1 p ro tein; P < 0. 05ΓΚ9. 23 U ·m g - 1 p ro tein; P < 0. 05Γin the act ivit ies of SOD and CA T com 2
pare w ith B group Μ12 s m icrow ave radia t ion of
UV 2B 2induced seedlings w ere on ly cau se a sign if i2
can t imp rovem en t of 1. 68 U ·m g- 1 p ro tein ; P <
0. 05ΓΚ8. 04 U ·m g- 1 p ro tein ; P > 0. 05Γin the ac2
t ivit ies of SOD and CA T respect ively.
A ct ivit ies of POD w ere declined rap id ly by
UV 2B radia t ion compared w ith con tro l ; F ig. 6 ΓΚ
w h ich 4. 17 U ·m g- 1 p ro tein; P < 0. 05Γlow er than
that of CK. O n the con traryΚw hen UV 2B treated
seedlings w ere expo sed to 3 s m icrow ave radia t ionΚ
act ivit ies of POD w as 3. 89 U ·g - 1 p ro tein ; P <
0. 05Γh igher than that of UV 2B radia t ion aloneΜex2
po sed to 6 s m icrow ave radia t ionΚPOD act ivit ies
w as 4. 83 U ·m g- 1 p ro tein ; P < 0. 01Γh igher than
that of UV 2B radia t ion aloneΜ9 s m icrow ave radia2
t ion of UV 2B 2induced seedlings cau se a sign if ican t
imp rovem en t of 2. 21 U ·m g- 1 p ro tein ; P > 0. 05Γ
in the act ivit ies of POD compare w ith B group Μ12 s
m icrow ave radia t ion of UV 2B 2induced seedlings
w as on ly cau se a sign if ican t imp rovem en t of 1. 11
U ·m g- 1 p ro tein; P < 0. 05Γin the POD act ivit ies.
F ig. 6 POD activit ies of the seedlings in ju red by en2
hanced UV 2B radiat ion in treatm ents of m icrow ave ra2
diat ion
3 D iscu ssion
T he resu lts from m uch of the rela ted research
indica te that enhanced UV 2B radia t ion can adverse2
ly influence the physio logica l and b iochem ical p ro2
cess of p lan ts[4 ]. P reviou s studies have demon stra t2
ed that po ten t ia l key target fo r UV 2B dam age is
m em b ranesΚDNA and o thers m icromo lecu lar [18 ].
W hen p lan ts w ere expo sed to elevated UV 2B irra2
dia t ion sΚthe b iom em b rane is in ju red and allow s
ion s such as K+ ΚN a+ inf ilt ra te [19 ]Κresu lt ing in the
decrease of the un satu ra ted fa t ty acids ra t io and
the mob ility of the cell m em b rane [18 ]Κlead to p ro2
duce free radica ls in p lan ts[20 ]. A s the resu ltΚthe
concen tra t ion of M DA Κas an indica to r of lip id per2
322211 期 陈怡平Κ等Π低剂量微波辐射对增强UV 2B 辐射损伤菘蓝幼苗抗氧化酶活性的影响;英Γ
ox idat ionΚ is increased and p lan t cells are in2
ju red [21 ]. T herefo reΚhow to decrease the concen tra2
t ion of M DA is very impo rtan t th ing fo r reducing
dam age of elevated UV 2B radia t ion p lan ts. T here
are a few w ays to reso lve th is p rob lem Κsuch as in2
creasing superox ide dism udase ; SOD ΓΚperox idase; POD Γ and cata lase ;CA T Γ act ivityΚA sA concen2
t ra t ion and UV 2B ab so rb ing compounds in p lan ts.
P resen t lyΚsom e study indica ted that laser rad ia t ion
also ach ieve th is a im [22 ]. In th is studyΚou r resu lts
p resen ted here the concen tra t ion of M DA w ere in2
creased sign if ican t ly w hen seedlings w ere expo sed
to enhance UV 2B radia t ionΚw h ich suppo rt the hy2
po thesis tha t cellm em b rane is a key target fo r UV 2
B radia t ion [18 ]. How everΚenhanced UV 2B radia t ion
fo llow ed m icrow ave radia t ionΚthe resu lt of a no2
tab le decrease of M DA concen tra t ion w as ob2
served. W en t w ithou t sayingΚthe m icrow ave is ab le
to recovery the in ju red cellu lar m em b rane and re2
pair p lan t dam age by UV 2B radia t ion.
T he p rob lem is w hat pathw ays w ere u sed by
m icrow ave to repair the UV 2B dam age. In ch lo ro2
p lastsΚSOD ΚCA T and POD are regarded as key en2
zym es of the scavenging pathw ayΚand UV 2B radia2
t ion is know n to induce a decrease in their act ivi2
t ies[23 ]. In th is studyΚact ivit ies of SOD ΚCA T and
POD in the fou rth leaves of seedlings w ere sign if i2
can t ly decreased by UV 2B radia t ionΚju st as above
repo rted. How everΚafter the UV 2B dam age in the
seedlings fo llow ed m icrow ave radia t ionΚtho se an2
t iox idat ive enzym es act ivit ies w ere sign if ican t ly in2
creased. M eanw h ileΚthe M DA concen tra t ion s w ere
no tab ly decreased w ith the increasing of an t iox ida2
t ive enzym es act ivit ies. It w as w ell know n that
tho se an t iox idat ive enzym es cou ld elim inate free
rad ica ls and p reven t lip id perox idat ion [23 ]. T here2
fo reΚm icrow ave m ay part icu lar in the repair effect
of seedlings in ju red by UV 2B radia t ion th rough im 2
p roving act ivit ies of som e an t iox idat ive enzym es.
T he nex t p rob lem is w hether the repair effect
of m icrow ave w as associa ted w ith increase ex2
p ressed of an t itox ican t genes. In recen t yearsΚsom e
studies abou t hT ER T 2R PE1 show n that m i2
crow ave radia t ion can ra ise the exp ress of tox icide
genes[24 ]. UV 2B expo sed cou ld increase UV 2B ab2
so rb ing compo
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