面口部穴位调节内脏功能的中枢机制(1)

nullC-fos expressions in the nucleus of the solitary tract following electroacupuncture at orofacial acupoints and gastric distensionC-fos expressions in the nucleus of the solitary tract following electroacupuncture at orofacial acupoints and gastric distension By Jianhua LiuKey wordsKey wordsc-fos nucleus of the solitary tract (NTS) medial subnucleus of the solitary tract (mNTS) electroacupuncture (EA) orofacial acupoints gastric distension convergence ContentContentIntroduction Methods Results Discussion Conclusions AcknowledgementsIntroductionIntroductionOrofacial acupointsStomachNTSAcupuncturetreating corresponding organic diseasesan important primary nervous center in modulating visceraltreating local orofacial diseases MethodsMethodsControl group EA at Sibai acupoints group EA at Yangbai acupoints group EA at Jiache acupoints group Gastric distension groupAnimalsTechniqueimmunohistochemistryFig.1. Location of the Sibai,Yangbai and Jiache acupoints in orofacial areasFig.1. Location of the Sibai,Yangbai and Jiache acupoints in orofacial areasResultsResultsC-fos expression in the NTS EA at orofacial acupoints induced significantly c-fos expression in the mNTS the portion of C-fos expression in the NTS following EA at orofacial acupoints and gastric distension is overlappedFig.2. draft of the NTS in the medull aoblongata (at the level of the area postrema ) Fig.2. draft of the NTS in the medull aoblongata (at the level of the area postrema ) Fig.3. Photomicrographs of the NTS at the level of the area postrema AP=area postrema, NTS=nucleus of the solitary tract, CC=central canal, DMV=dorsal motor nucleus of the vagus nerve, ts=tractus solitarius, c=commissural subnucleus of the NTS, m=medial subnucleus of the NTS, im=intermediate subnucleus of the NTS, dm=dorsomedial subnucleus of the NTS, vl=ventrolateral subnucleus of the NTS. Scale bars100 mm. Fig.3. Photomicrographs of the NTS at the level of the area postrema AP=area postrema, NTS=nucleus of the solitary tract, CC=central canal, DMV=dorsal motor nucleus of the vagus nerve, ts=tractus solitarius, c=commissural subnucleus of the NTS, m=medial subnucleus of the NTS, im=intermediate subnucleus of the NTS, dm=dorsomedial subnucleus of the NTS, vl=ventrolateral subnucleus of the NTS. Scale bars100 mm. Fig.4. Photomicrographs of C-fos expression in the NTS at the level of the AP ( EA at Sibai acupoints group) Fig.4. Photomicrographs of C-fos expression in the NTS at the level of the AP ( EA at Sibai acupoints group) Fig.5. Photomicrographs of C-fos expression in the NTS at the level of the AP ( EA at Yangbai acupoints group) Fig.5. Photomicrographs of C-fos expression in the NTS at the level of the AP ( EA at Yangbai acupoints group) Fig.6. Photomicrographs of C-fos expression in the NTS at the level of the AP ( EA at Jiache acupoints group) Fig.6. Photomicrographs of C-fos expression in the NTS at the level of the AP ( EA at Jiache acupoints group) Fig.7. Photomicrographs of C-fos expression in the NTS at the level of the AP ( gastric distension group) Fig.7. Photomicrographs of C-fos expression in the NTS at the level of the AP ( gastric distension group) Fig.8. Photomicrographs of C-fos expression in the NTS at the level of the AP ( control group) Fig.8. Photomicrographs of C-fos expression in the NTS at the level of the AP ( control group)DiscussionDiscussionPrevious studies have shown that orofacial noxious information induced by formalin and electrical stimulation resulted in the c-fos expression in the medulla oblongata. However, the particular distribution of c-fos in the NTS has not been further investigated.nullIn the present study, we found that EA at the orofacial acupoints, which were located in the areas of different branches of trigeminal nerves, all induced the c-fos expression in the mNTS, which may be caused by two different pathway. The afferent nerves of branches of the trigeminal nerves can project directly and indirectly into the NTS, therefore, the fos-like neurons in the NTS may be reactive to the afferent sensory information from different orofacial areas. nullFurthermore, the distribution of c-fos expression in the mNTS after gastric distension was similar to that induced by EA stimulation, which suggested that some specific neurons in the NTS may be simultaneously reactive to the information from the orofacial areas and the stomach and this may be one of the central pathways in the modulation of EA at orafacial acupoints on the gastric functions. ConclusionsConclusionsSensory afferent information from the orofacial areas and the viscus (stomach) may be converged in the mNTS The convergence my be involved in the modulation of EA at the orofacial acupoints on the stomachAcknowledgementsAcknowledgementsThis work was supported by the National Natural Science Foundation of China (No 30200356) Thank Mr Li jiangshan for his excellent technical assistance.Thanks for Your AttentionThanks for Your Attention