、NCBI上下载基因序列、mRNA、CDS序列的方法1.打开NCBI网站,第一个框选择Gene,第二个框输入基因名称,如ALK基因,点击Search。2.进入第二页面后,会看到如下一系列跟ALK基因相关的信息,根据description和location的信息,找到自己需要的基因。空色框中
示的是物种,我需要的是人类的基因,故选择第一个。点击八1(进入下一个页面。SearchresultsIteMS:1to20Of2651«First
Last»OSee3信口73discontinuHd酬rgHaceditems.Name/GeneIDDescriptionLocationAliasesMIM□ALKwnmpEsticIvmphoFniareceptortyrosineChromosome2,NC_DDQ002.1I2CD246,NBLST3105590ID:238kin召sj[Home电加(huimar)]|(29192774...2992161kcomplement}□manaplasticlymphomiakinase[MusChromosome17:NC_OOD083.6CD246,TcrzID:11632mtssculus(housemouse)](71B6898a..7260^648,complennent]□AikArapIasticlymphomakinaseChromosome2RNT_033778.4Dmel_CGS250:ALK,CG825C:DAIk,ID;53425[Drasophi'arne'anogaster(fruitfly)"(16625291.16640011complement)DAlk53:Dmel\CG8250,alk,dALK,mili3.进入后,会看到以下关于ALK基因的详细信息:修NCBIResources©Howitt叵GeneGeneSearchAdvancedOfficialSymbolOfficialFullNamePriiriiarysourceSeerehtBdGenetypeRefSeqstatusOrganismLineageAis口knownasSummarySendio.▼FullRepaitTALKanaplasticlymphomareceptortyrosinekinase[Homosapiens(huinan)]GeneID:238,updatedan6-Dec-2016上SummaryALKLyTGHEanaplasticlymphomareceptortyrosinekinasepuxidcdbj卜RM/□laerublENSG口「口口了门"4MIMR555。,延即OTTHUMGUOODDISSDWproteincodingREVIEWEDHono5/pie~n:5Eukaryota;Metazoa;Chordata:Crianiata;Vertebrata;Euteleostomi;Mammalia;Eutheria:Euarchortoglires;Primates;Haplorrhini;Catarrhini:Hominidae:HomoCD246;NBLST3Thisgenesncod^Gareceptartyrosinekinass,whicht>slDingstotheinsulinreceptorsuperfamily.ThisprotsincomprisesanieKiracellulardomain,anhydrophobicstretchcorrespondingtoa&ingl@passtransmembraneregion,andanintracsluiarkinasedomain.Itplar/samimportailrolsinthedlevslopimientofthebrainandexertsits-affectsnrcncij%rtfiirrusiiimncinFhan口mv四uicleTinicmonoIn口ciKaonfriiinrihnh口arnroninaHm«i+s+odnramrihfiicirlin□eorinc.n.ftiimdniiremr-liudlinnnFifanl口lornordI这些信息可以略过,往下查看,点击下图红色框中的SeeALKinGenomeDataViewer,进入下个页面。GenomeDataViewertPkk砧阳丽卡SefertanagserriXytocrangelleoaramViewQO-,二-WIHUnlHomosapiens:GRCh33.p»(GCFJOT0004406.3S)Chr2^NC_ODJ>OOZ12|:29.12M-2999M15ORB「runr-..-rrrm,--标准序列的话rmRNA就采用NM_开头的,基因组用NC_或者AC_开头的口soeasy1NP_123456NP_1234567S9ProteinMixed磨白声勤;主要是仝长转录氨窗序列,但也有T只有部分蛋白质的部分氨基辞序列,NR_123456RNAMixed本编码的转录子序列,包恁给构RNAs,依基因能子等。NT_123456GenomicAutomated日A避者马粕则序法的还未完全注释的测监序,卜WW_123456GenomicAutomatedEAC或者鸟粕加序法的还未完全注释的测喙序列;■NVt_12345fi789NZ_ABCD1234567BGenomicAutomated收隼的告种利用马枪法测序的测脖
,ABCB代去的是计划的名称。一―号—工忆