流式细胞学技术抗体的各种荧光标记颜色及波长及所占用通道

Fluorochromes for Flow Cytometry and Microscopy Fluorochrome Excitation Max (nm) Excitation Laser Lines (nm) Emission Max (nm) BD FAC- Scan™ BD FAC- SCalibur™ BD FACSVan- tage™SE BD FAC- SCanto™ BD FAC- SCanto™ II BD™ LSR II BD FAC-SAria™ Beckman Coulter® EPICS® XL™/ XL-MCL™ Beckman Coulter® FC 500 CYTOPEIA inFlux™ Dako CyAn™ ADP Dako MoFlo ® Guava EasyCyte iCyt Reflection® Accuri C6™ Flow Cytometer Amnis ImageStream® Fluorochrome Cascade Blue® 377 360, 405, 407 420 DAPI DAPI DAPI FL-1 DAPI FL-6 DAPI/PacBlue Channel 2 Cascade Blue® Alexa Fluor® 405 401 360, 405, 407 421 DAPI DAPI DAPI FL-1 DAPI FL-6 DAPI/PacBlue Channel 2 Alexa Fluor® 405 Pacific Blue™ 410 360, 405, 407 455 DAPI DAPI DAPI FL-1 DAPI FL-6 DAPI/PacBlue Channel 2 Pacific Blue™ AmCyan 458 405, 407, 457 489 DAPI DAPI DAPI FL-1 DAPI FL-6 DAPI/PacBlue Channel 2 AmCyan Cascade Yellow™ 400 405, 407 550 Pac. Orange Pac. Orange Pac. Orange FL-2 Pac. Orange FL-7 Pac. Orange Cascade Yellow™ Pacific Orange™ 400 360, 405, 407 551 Pac. Orange Pac. Orange Pac. Orange FL-2 Pac. Orange FL-7 Pac. Orange Pacific Orange™ Alexa Fluor® 488 495 488 519 FL-1 FL-1 FITC FITC FITC FITC FL-1 FL-1 FL-3 FITC FL-1 PM-3 FITC FL-1 Channel 3 Alexa Fluor® 488 FITC (fluorescein) 493 488 525 FL-1 FL-1 FITC FITC FITC FITC FL-1 FL-1 FL-3 FITC FL-1 PM-3 FITC FL-1 Channel 3 FITC (fluorescein) PE (R- Phycoerythrin) 496, 565 488 575 FL-2 FL-2 PE PE PE PE FL-2 FL-2 FL-4 PE FL-2 PM-1 PE FL-2 Channel 4 PE (R- Phycoerythrin) Cy3 514 488, 514 566 FL-2 FL-2 PE PE PE PE FL-2 FL-2 FL-4 PE FL-2 PM-1 PE FL-2 Channel 4 Cy3 PE/Cy5 496, 565 488 670 FL-3 FL-3 PE/Cy5 PE/Cy5 PE/Cy5 FL-4 FL-4 FL-6 PE/Cy5 FL-4 PM-2 PE-Cy5 FL-3 Channel 6 PE/Cy5 PerCP 482 488 675 FL-3 FL-3 PE/Cy5 PE/Cy5 PE/Cy5 FL-4 FL-4 FL-6 PE/Cy5 PM-2 PE-Cy5 FL-3 Channel 6 PerCP PE/Cy5.5 496, 565 488 690 FL-3 FL-3 PerCP/Cy5.5 PerCP/ Cy5.5 PerCP/Cy5.5 PerCP/ Cy5.5 FL-4 FL-4 FL-6 PE/Cy5 FL-4 PM-2 PE-Cy5.5 FL-3 Channel 6 PE/Cy5.5 PerCP-Cy5.5 482 488 690 FL-3 FL-3 PerCP/Cy5.5 PerCP/ Cy5.5 PerCP/Cy5.5 PerCP/ Cy5.5 FL-4 FL-4 FL-6 PE/Cy5 PM-2 PE-Cy5.5 FL-3 Channel 6 PerCP-Cy5.5 PE/Texas Red® 496, 565 488 613 FL-2 PE PE PE FL-3 FL-3 FL-5 PE/Texas Red FL-3 PE-TR Channel 5 PE/Texas Red® PE/Cy7 496, 565 488 774 FL-3 FL-3 PE/Cy7 PE/Cy7 PE/Cy7 PE/Cy7 FL-5 PE/Cy7 FL-5 PM-4 PE-Cy7 FL-3 PE/Cy7 APC (allophycocyanin) 645 595, 633, 635, 647 660 FL-4 APC APC APC APC FL-4 FL-7 APC FL-8 APC FL-4 Channel 6 APC (allophycocyanin) Cy5 649 633, 635 666 FL-4 APC APC APC FL-4 FL-7 APC FL-8 APC FL-4 Channel 6 Cy5 Alexa Fluor® 647 650 595, 633, 635, 647 668 FL-4 APC APC APC FL-4 FL-7 APC FL-8 APC FL-4 Channel 6 Alexa Fluor® 647 APC/Cy5.5 650 595, 633, 635, 647 690 FL-3 APC/Cy5.5 APC/Cy5.5 APC/Cy5.5 APC/Cy5.5 FL-4 FL-7 APC/Cy5.5 FL-4 APC-Cy5.5 FL-3 Channel 6 APC/Cy5.5 Alexa Fluor® 700 696 633, 635 719 APC/Cy5.5 APC/Cy5.5 APC/Cy5.5 FL-4 FL-7 PE/Cy5 FL-4 APC-Cy5.5 Channel 6 Alexa Fluor® 700 APC/Cy7 650 595, 633, 635, 647 774 APC/Cy7 APC/Cy7 APC/Cy7 APC/Cy7 FL-5 FL-8 APC/Cy7 FL-9 APC-Cy7 APC/Cy7 APC/Alexa Fluor® 750 650 595, 633, 635, 647 774 APC/Cy7 APC/Cy7 APC/Cy7 APC/Cy7 FL-5 FL-8 APC/Cy7 FL-9 APC-Cy7 APC/Alexa Fluor® 750 Viability Probe Viability Probe DAPI 359 325, 360, 405, 407 461 DAPI DAPI DAPI FL-6 DAPI FL-6 DAPI/PacBlue Channel 2 DAPI 7-AAD (7- aminoactinomycin D) 546 488 647 FL-3 FL-3 PerCP/Cy5.5 PerCP/ Cy5.5 PerCP/Cy5.5 PerCP/ Cy5.5 FL-3 FL-3 FL-4 PE/Cy5 FL-4 PM-2 PE-Cy5 FL-3 Channel 5 7-AAD (7- aminoactinomycin D) PI (Propidium Iodide) 305, 540 325, 360, 488 620 FL-3 FL-3 PerCP/Cy5.5 PerCP/ Cy5.5 PerCP/Cy5.5 PerCP/ Cy5.5 FL-3 FL-3 FL-4 PE/Cy5 FL-4 PM-1 PE-TR FL-2 Channel 5 PI (Propidium Iodide) Trademarks: BD FACScan™ , BD FACSCaliber™, BD FACSVantage™ SE, BD FACSCanto™, BD™ LSRII, BD FACSAria™ are trademarks of Becton, Dickinson and Company. Coulter® EPICS® XL™/XL-MCL™ are registered trademarks of Beckman Coulter, Inc. inFlux™ is a trademark of Cytopeia, Inc. iCyt Reflection® is a registered trademark of iCyt Mission Technology, Inc. CyAn™ ADP and MoFlo™ are trademarks of Dako Denmark A/S. C6™ is a trademark of Accuri. Image Stream® is a registered trademark of Amnis Corporation. Cascade Blue®, Alexa Fluor® , Texas Red® are registered trademarks of Molecular Probes, Inc. Pacific Blue™ is a trademark of Molecular Probes, Inc. * These fluorochromes can be used as single color configurations or multi-colored configurations depending on instruments, available lasers and filters, and appropriate color compensation settings. ** These instruments are provided as general information. User must check instrument capability and choose appropriate setting. BioLegend 11080 Roselle St. San Diego, CA 92121, USA Toll-free Phone: 1-877-246-5343 Phone: (858) 455-9588 Fax: (858)455-9587 Email: customerserv@biolegend.com, techserv@biolegend.com asus 矩形 asus 矩形 asus 矩形 asus 矩形 asus 矩形 asus 矩形 asus 矩形