一种可反复在小鼠身上取血的方法——颌下静脉取血法

一种可反复在小鼠身上取血的方法——颌下静脉取血法 小鼠颌下颌采血法静脉 在物研究中动动动动动动动, 特是生理学、理学、动动动动动动动动动动动物代力学等研究中动动动动动动动动, 动动动动动动动动动动动动动动动常要采集物的血液本行常 量、胞学或行生理、生化分析动动动动动动动动动动动动动动动 动动。目 前, 小鼠常用的采血方法有眼眶后静脉采血法、眼球动动动动动动动摘除采血法、心采血法、剪尾采血法等动动动动动动动动动动动 , 不同的采血方法适用于不同的用途和所需血量。动动动动动动动动动动动动动动解剖、察小鼠部浅血管动动动动动动, 动动动动动动动动动动动动动动动动其下部静脉血管丰富、位置浅, 动动并建立了小鼠下静脉采血的新方法。方法动动动动动动动动动动动动动动动 无需麻醉、小、采血量大并可重采血动动动动动动动动动动动动动动, 是一安全、动动动动快速、便、采血量多的采血方法。动动动动动动动动动动动动动 已有的众多采血方法各有其缺点。例如动动动动动动, 动动于体重25 g 左右的成年小鼠, 眼眶后静脉采血法采血量比多动动动动动动动动动动, 动动0?2~ 0.3 mL, 并可重采血动动动,但易眼球动动动动; 眼球摘除采血法采血量多, 动动0.3~ 0.4 mL, 但不可重采血动动动, 而且可致小鼠失明, 易感染, 止血不动动动可致小鼠死亡, 同也不符合物福利基本原动动动动动动动动动动动动动; 心采血动动动法采血量可达0.8 mL,但由于小鼠个体小、心小、心搏快动动动动动动动动, 采血位置固定动动动动动动动 , 因而操作度系数大动动动动动, 需要麻醉, 且容易因操作致物死亡动动动动动动动动; 剪尾采血法, 动动动动动动动可以多次采血, 但每次采血量极少, 不到0.1 mL, 不适于需要多血量的动动动动动动动 动动动动下静脉去学:步 1、徒手固定法: 将小鼠固定在左手中。如动1 2、在小鼠部下部准确把握下静脉血管位置动动动动动动动动动动动动动动动动动动, 如动2 中箭所指位置动动动动动, 血管位于下骨后方咬肌。酒动动动动动动动动动动精棉球擦拭采血部位消毒后, 将菌注射迅速刺入动动动动动动动动动动动动动动下静脉血管( 动3) , 血液即流出( 动4) 。采血束动动, 立即用动菌干棉球迫止血。动动动动动 3、将血液收集于菌离心管内动动动动动动, 动动动号用 小鼠常用取血方法比较 采血断尾眼眶动下静眼球腋动心动断动方法静脉脉摘除脉穿刺 0.1-0.20.2-0.30.3-0.50.2-0.30.3-0.50.5-0.80.7-1.1采血 量/ml 操作易动中中中动易动度 用2-41-1.50.5-1.512-31-31-1.5动/mi n 能否能能能动能重一般偶动不能重动动一次不能不能取血