乳酸脱氢酶（LDH-L）液体试剂盒说明书

乳酸脱氢酶（LDH-L）液体试剂盒说明 Lactate Dehydrogenase Assay Kit (LDH-L) L?P Cat. 2050: R1 (60ml×3) + R2 (54ml×1) 2550: R1 (80ml×3) + R2 (72ml×1) 8050: R1 (45ml×30 + R2 (40ml×1) Precautions and warnings: Intended Use: 1. When ?A/min?0.130, dilute the sample with 0.9% NaCl and For the quantitative determination of LDH-L in human serum re-assay it.. Assay Principle: 2. Discard the kit when the Blank Absorbance is over 0.6. LDH ++ 3. Sodium Azide included. Avoid ingestion or contact with skin. In L-lactic acid + NAD Pyruvic acid + NADH + Hcase of skin contact, flush with copious amounts of water and wash with soap and water for at least 15 minutes. If swallowed, Reagents Composition: wash mouth with water provided person is conscious. Call a Reagent Components Concentration physician or poison control. 4. Shall be stored at 2…8?. This product is stable until the Tris buffer(pH8.9) 100mmol/L expiration date indicated on the label under the specified R1 KCl 100mmol/L storage conditions. L-Lithium lactate 52mmol/L R2 NAD 6.5mmol/L Bibliography: 1. Bergmeyer: Methods of Enzymatic Analysis. Third Edition, Vol.?:126~132 Stability: 12 months at 2,8?, protecting from light. Parameter when applied on Hitachi analyzers: Sample: 7170 7060 7150 Fresh Serum，heparinized plasma. Assay Code RATE-A RATE-A RATE-A Note: Strictly avoid using hemolysis sample. Reaction time 10 10 — Assay Point 22~28 21~27 30~40 Assay Conditions: Wavelength 405/340 405/340 405/340 Wavelength:340nm Sample Volume 5 7 7 Temperature:37? R1 Volume 200 250 250 Cuvette:1cm R2 Volume — — 75 Procedures: R3 Volume 60 75 — R4 Volume — — — Ready For Use ABS.Limit 25000 25000 25000 Blank Sample INC/DEC INC INC INC Sample — 24μl Calib.Type Linear Linear K Factor Water 24μl — Calib.Point 1 1 — R1 900μl 900μl Span.Point 0 0 — Mix，stand at the appropriate temperature for 5 minutes SD Limit 0.1 0.1 0.1 R2 270μl 270μl Duplicate Limit 500 500 500 SI ABS Limit -500~5000 -500~5000 — Mix，stand at the appropriate temperature for 1 minutes，Unit U/L U/L U/L read the absorbance ?A for 1 minute. STD(1)Conc 0 0 0 Calculation: STD(2)Conc 0 0 0 Expected Value 109~245 109~245 109~245 LDH-L(U/L) = (?A/min,?A/min)×F samplecontrolK Factor 8413 7528 7528 V t F = ×1000 = 7897 V×K sRegistration number: 3400102-2004 V= reaction volume, V= sample volume t s Executed Standard: YZB/GB 2082-2003 NADPH under 340nm wavelength K =6.3 Reference value: Biosino Bio-Technology and Science Inc. 109~245U/L Add:No.27, Chaoqian Road, Changping District, Note: The reference range should be determined by each hospital to 102200 Beijing, China confirm with the characteristics of the region being tested. Tel:+86-10-69722840 Linearity: Fax:+86-10-69722840 The test is linear up to 1000U/L. Website:www.zhongsheng.com.cn Parameters 400 560 HITACHI 7020 600 800 OLYMPUS AU 1 TEST LDH-L 640 1000 2 METHOD RATE A TEST LDH-L LDH-L 4 REACTION TIME 10 SAMPLE VOL. 5 7 5 MEASURING POINT 1 22 DILUTION 0 0 6 MEASURING POINT 2 28 R1 REAGENT VOL. 200 250 9 WAVELENGTH-2 405 DILUTION 0 0 R2 REAGENT VOL. 60 75 10 WAVELENGTH-1 340 DILUTION 0 0 11 SAMPLE VOL. 5 WAVELENGTH-1 340 340 12 REAGENT 1 VOL. 200 WAVELENGTH-2 410 410 15 REAGENT 2 VOL. 0 METHOD RATE RATE 18 REAGENT 3 VOL. 60 REACTION SLOPE + + MEASURING POINT 1:FIRST 15 11 21 CAL. TYPE K FACTOR MEASURING POINT 1:LAST 21 16 23 CAL.1 CONC. 0 NO LAG-TIME YES YES 24 CAL.1 POS. 99 PROZONE CHECK NO NO 25 CAL.2 CONC. - REAGENT OD L -0.1000 -0.1000 36 K FACTOR 8413 H 0.5000 0.5000 45 DUPLICATE LIMIT 500 MIN-OD 0.0000 0.0000 MAX-OD 2.0000 2.0000 49 ABS. LIMIT 25000 LINEARITY % 30 30 50 REACTION DIR.(I/D) I UNIT U/L U/L 56 INSTRUMENT 1.0 CORRELATION FACTOR CONSTANT a A= 1.0000 1.0000 57 INSTRUMENT B= 0.0000 0.0000 0 FACTOR 8413 7528 CONSTANT b