花椒化学成分

73RESEARCH CHILEAN JOURNAL OF AGRICULTURAL RESEARCH 71(1):73-82 (JANUARY-MARCH 2011) CHEMICAL COMPOSITION, INSECTICIDAL, AND ANTIFUNGAL ACTIVITIES OF FRUIT ESSENTIAL OILS OF THREE COLOMBIAN Zanthoxylum SPECIES Juliet A. Prieto1*, Oscar J. Patiño1, Wilman A. Delgado1, Jenny P. Moreno1, and Luis E. Cuca1 ABSTRACT This study determined the chemical composition of essential oils isolated from Zanthoxylum monophyllum (Lam.) P. Wilson, Z. rhoifolium Lam., and Z. fagara (L.) Sarg. fruits by steam distillation, as well as testing antifungal and insecticidal activities of essential oils as potential pesticides. Gas chromatography-mass spectrometry (GC/MS) analysis identified 57 compounds. The main constituents in Z. rhoifolium oil were β-Myrcene (59.03%), β-phellandrene (21.47%), and germacrene D (9.28%), the major constituents of Z. monophyllum oil were sabinene (25.71%), 1,8-cineole (9.19%), and cis-4-thujanol (9.19%), whereas fruit oil of Z. fagara mainly contained germacrene D-4-ol (21.1%), elemol (8.35%), and α-cadinol (8.22%). Zanthoxylum fagara showed the highest activity on Colletotrichum acutatum Simmonds (EC50 153.9 μL L-1 air), and Z. monophyllum was the most active against Fusarium oxysporum Schlechtend: Fr. f. sp. lycopersici (EC50 140.1 μL L-1 air). Zanthoxylum monophyllum essential oil showed significant fumigant activity against Sitophilus oryzae (L.). This study demonstrated that Zanthoxylum essential oils exhibit important fungicidal activity on F. oxysporum and C. acutatum, which could become an alternative to synthetic fungicides to control plant fungal diseases, and Z. monophyllum oil is a potential fumigant against S. oryzae. Key words: Zanthoxylum monophyllum; Z. rhoifolium; Z. fagara; Sitophilus oryzae; Fusarium oxysporum f. sp. lycopersici; Colletotrichum acutatum; fumigant, antifungal, insecticidal. 1Universidad Nacional de Colombia, Facultad de Ciencias, Bogotá, KR 30 45 03, Colombia. AA 14490. *Corresponding author (japrietor@unal.edu.co, japrietor86@gmail.com). Received: 7 June 2010. Accepted: 24 September 2010. INTRODUCTION Crop losses due to insects and plant diseases caused by fungi, bacteria, and viruses cause significant economic losses (Kotan et al., 2008; Kordali et al., 2008). Insect pests often cause extensive loss of products stored in tropical and semitropical environments (Isman, 2000). For example, Sitophilus species are serious cosmopolitan pests of stored grain (Liu and Ho, 1999). Sitophilus zeamais Motschulsky (maize weevil), S. oryzae L. (rice weevil), and S. granaries L. are the main representatives of this genus, which principally attack rice (Oryza sativa L.), maize (Zea mays L.), wheat (Triticum sativum Lam.), and sorghum (Sorghum bicolor (L.) Moench) among others, through direct feeding on grain kernels causing unfavorable effects on food quality, safety, and preservation (Huang et al., 1997; Tapondjou et al., 2002; Kim et al., 2003; Park et al., 2003; Arannilewa, 2007). Harvest losses due to fungal disease in world crop production may amount to 12% or more in developing countries (Horbach et al., 2010). Many pathogens including Fusarium oxysporum (vascular wilt), F. solani (fruit rot) and Colletotrichum gloeosporoides (fruit rot) cause severe pre- and post-harvest damage to agriculture (Bajpai et al., 2008). Fusarium is a plant pathogen that causes wilt diseases of several economically important plants and is also known to produce toxins thought to contribute to wilting by affecting cell membrane permeability and disrupting cell metabolism (Garcés de Granada et al., 2001; Pawar and Thaker, 2007). Colletotrichum are pathogens that cause anthracnose in a wide range of woody and herbaceous crops. Symptoms are broad-ranging and include stem rot, dieback, and seedling blight. Fruits are affected during the pre- and post-harvest period (Roca et al., 2003; Muñoz et al., 2009). Synthetic insecticides and fumigants are widely used to control grain pests and plant diseases. However, the indiscriminate application of synthetic products has led to various problems including toxic residues in treated products, environmental pollution, and resistance against 74 CHIL. J. AGR. RES. - VOL. 71 - Nº 1 - 2011 pesticides by microorganisms and grain insect pests (Huang et al., 1997; Isman 2006; Bakouri et al., 2008; Kotan et al., 2008; Ye et al., 2010). Therefore, because of increasing drawbacks of the continued use of conventional fumigants, an effort is needed to develop new alternative pesticides to replace those being currently used. Essential oils are potential botanical sources of alternative compounds to fumigants being currently used because of their low toxicity for warm-blooded animals, high volatility and toxicity for stored grain pests and plant microorganisms (Lee et al., 2001; Abad et al., 2007). Zanthoxylum genus (Rutaceae) is made up of about 250 species of trees and shrubs in the world’s tropical and temperate regions (Pirani, 1993). It is economically very important as a source of edible fruits, raw material for the cosmetics and perfume industries, as well as culinary applications. In Asia, Z. bungeanum Maxim. fruits are the most popular huajiao commercial product called “da hong pao’’ (big red robe). “Green huajiao”, fruit of Z. schinifolium Siebold & Zucc. (Yang, 2008), is the other widely used spice in Sichuan. Zanthoxylum species have shown significant insecticidal and antifungal activity. The bark methanol extract of Z. xanthoxyloides caused significant mortality rates in S. oryzae and Callosobruchus maculatus, two stored-product insect pests (Owusu et al., 2007). Zanthoxylum monophyllum bark methanol extract showed significant activity against seven human pathogen fungi (Gómez et al., 2007). Ethanolic extracts of Z. americanum leaves, fruits, stem bark, and root demonstrated a broad spectrum of antifungal and antibacterial activity against Candida albicans, Aspergillus fumigatus, Cryptococcus neoformans, and Fusarium oxysporum (Bafi-Yeboa et al., 2005). In addition to culinary applications, many species of Zanthoxylum are used in traditional medicine especially in America, Africa, and Asia. Zanthoxylum rhoifolium Lam is popular in South America for inflammatory, microbial, cancerous, and malaria processes (Da Silva et al., 2007a; 2007b). Zanthoxylum fagara is used in Cuba for the treatment of diarrhea, chest diseases, intermittent fever, earaches, and tooth diseases (Dieguez-Hurtado et al., 2003). Zanthoxylum monophyllum is used as an analgesic to treat nasal inflammation, jaundice, and eye infections in Venezuela and as colorant (Cuca et al., 1998; Díaz and Ortega, 2006; Da Silva et al., 2007a). Zanthoxylum species accumulate volatile oils in leaves, fruits, and inflorescences (Adesina, 2005). There are numerous reports on the chemical composition and the various biological activities of Zanthoxylum species essential oils (Choochote et al., 2007; Boehme et al., 2008; Yang, 2008). The chemical composition of essential oils of Z. rhoifolium flowers, fruits, and leaves (Gonzaga et al., 2003; Moura et al., 2006; Da Silva et al., 2007a; 2007b; Boehme et al., 2008), and biological properties of fruit and leaf essential oils, such as antibacterial and cytotoxic resultshave been previously reported (Moura et al., 2006; Da Silva et al., 2007b; Boehme et al., 2008). The volatile chemical composition of Z. monophyllum and Z. fagara leaves from Costa Rica have also been reported (Setzer et al., 2005). However, there are no studies about their biological properties. The composition and biological properties of fruit essential oils of Z. monophyllum and Z. fagara have not yet been investigated. We report the chemical composition, insecticidal activity against S. oryzae, and antifungal activity against F. oxysporum f. sp. lycopersici and C. acutatum of fruit essential oils of three Colombian plant species: Z. monophyllum, Z. fagara, and Z. rhoifolium. MATERIALS AND METHODS Plant material The fruits of Z. monophyllum (4º11’24.3’’ N, 74º30’48.9’’ W) and Z. fagara (4º11’22.0’’ N, 74º30’58.4’’ W) were collected in January 2008 in the town of Icononzo, Tolima, Colombia, whereas Z. rhoifolium fruits (4º19’30.4’’ N, 74º26’17.1’’ W) were collected in February 2008 in the town of Fusagasugá, Cundinamarca, Colombia. Plant samples were identified by the Colombian National Herbarium of the Universidad Nacional de Colombia. Voucher specimens of Z. monophyllum (Lam.) P. Wilson (COL-517520), Z. rhoifolium Lam. (COL-522896), and Z. fagara (L.) Sarg. (COL-522891) were deposited in the Colombian National Herbarium of the Universidad Nacional de Colombia, Bogotá, Colombia. Isolation of essential oils Samples of fresh fruits (approximately 2 kg) of each Zanthoxylum species were submitted to steam distillation (ca. 4 h). Oils were dried over anhydrous sodium sulfate and stored at 0-5 ºC for further analysis. Gas chromatography-flame ionization detector analysis (GC/FID) Volatile compound analysis was performed with a gas chromatography system (Hewlett Packard 5890 GC) with a fused capillary column (50 m × 0.25 mm × 0.25 μm, HP- 5MS, Crossbond 5% phenyl-95% dimethylpolysiloxane, Sigma-Aldrich, St. Louis, Missouri, USA) directly coupled to a flame ionization detector (FID). Injection conditions were the following: injector temperature at 250 ºC; oven temperature program at 45 ºC (2 min), 150 ºC (5 min) at a rate of 2 ºC min-1, then at 150 ºC (2 min), 280 ºC (5 min) at a rate of 8 ºC min-1; split 30:1 during 1.50 min, carrier gas He: 1 mL min-1, constant flow; sample volume 1 μL. 75J. PRIETO et al. - CHEMICAL COMPOSITION, INSECTICIDAL, AND…….. Gas chromatography-mass spectrometry analysis (GC/ MS) The GC/MS analyses were performed in electronic impact (EI) mode with a Hewlett Packard-5890 GC system with a fused capillary column (50 m × 0.25 mm × 0.25 μm, HP- 5MS, Crossbond 5% phenyl-95% dimethylpolysiloxane) directly coupled to a Hewlett Packard 5973 selective mass detector. Injection conditions were the same as described above. The mass spectrometer was operated at 70 eV. Oil component identification Oils were analyzed by GC-MS and GC with capillary columns (HP-5MS). Chemical constituents were identified based on the comparison of their mass spectral pattern and retention indices with those obtained from the Wiley 138.L, NBS 75K.L, and SDBS libraries, as well as those published by Adams (1995). Retention indices (RI) were calculated according to the literature (Van Den Dool and Kratz, 1963). Insect rearing Rice weevils were obtained from a colony maintained in the Laboratory of Entomology, Universidad Nacional de Colombia, Bogotá. Weevils were reared on maize grains. Cultures were maintained in the dark at 25 ± 1 °C and 70 ± 5% relative humidity. Fungal cultures Fusarium oxysporum f. sp. lycopersici was obtained from the culture collection of the Universidad de Cundinamarca (Laboratory of Phytopathology). Colletotrichum acutatum was obtained from the culture collection of the Universidad Nacional de Colombia, Bogotá (Laboratory of Vegetal Natural Products, Faculty of Science). Cultures were maintained and grown on potato dextrose agar (PDA) medium and incubated at 28 ± 1 °C. Insecticidal activity assay To determine the fumigant toxicity of Zanthoxylum oils, paper filter disks (Whatman Nº 1, 2-cm diameter pieces) were adhered to the inside of the Petri dish covers (90 x 15 mm) and then impregnated with oil at doses calculated to give equivalent fumigant concentrations of 242-967 μL L-1 air (20, 40, 60, and 80 μL oil). Twenty adult insects (1 to 10-d-old) were placed on each Petri dish. Phostoxin®-Fugran (phosphine - 300 μg L-1 air) and Nuvan® 50 E.C. (clorvox - 100 μL L-1 air) were used as positive controls. Petri dishes were sealed with Parafilm and incubated at 25 ± 1 °C and 70 ± 5% RH. Each concentration and control was replicated three times. Mortality was determined after 12, 24, and 48 h from the start of exposure (Negahban et al., 2007; Kotan et al., 2008). Insect mortality percentage (%M) was calculated by Abbott’s correction formula (Pitasawat et al., 2007). LC50 and LC95 values, as well as the corresponding 95% confidence intervals, were estimated by probit analysis (Finney, 1971). In vitro antifungal activity PDA plates were prepared with glass Petri dishes (90 x 15 mm) for the in vitro antifungal activity test. Agar plugs of actively growing cultures in PDA were placed on one half of the Petri dish (covered with PDA) and a sterilized paper disk was placed 2 cm from them. A 10 μL aliquot of each essential oil was added to the paper disks in each of the PDA plates (maximum 5 μL per disk). Plates were immediately sealed with Parafilm after adding each essential oil and incubated for 3 d at 28 °C. The diameter of concentric fungal mycelia was measured and compared with the untreated control. Medium effective concentration (EC50) values were determined for essential oils that caused fungal growth inhibition. Aliquots of 2, 5, 7, 10, and 15 μL (23.5 - 176.5 μL L-1 air) of each essential oil were added to paper disks in each of the PDA plates (maximum 5 μL per disk). Plates were replicated three times in each treatment. (Lee et al., 2007; Kotan et al., 2008). In addition, Benlate 50WP (methyl- [1-[(butylamino) carbonyl]-1H-benzimidazol-2-yl] carbamate - 2 mg mL-1) and Derosal®-Bayer (methyl-1H- benzimidazol-2-ylcarbamate - 1 mg mL-1) were employed as chemical controls in the F. oxysporum f. sp. Lycopersici and C. acutatum assays, respectively. Statistical analysis Data are presented as mean ± standard error. Statistical significance was determined by the Duncan and Tukey tests; ANOVA determined whether results obtained for antifungal and insecticidal activity assays were statistically different. Statistical significance was set at P < 0.05. RESULTS AND DISCUSSION Oil chemical composition Fifty-seven compounds were identified by gas chromatography and mass spectrometry data in the fruit essential oils of three Zanthoxylum species (Table 1). Oils mainly contain monoterpenes and sesquiterpenes. Identified volatile components accounted for 89 to 99% of oil composition. Monoterpenes represent more than 70% of Z. rhoifolium oil composition (80.5%), Z. monophyllum (71.6%), and only 6.16% of Z. fagara oil composition. Sesquiterpenes represent 88.8% of Z. fagara oil composition. Chemical profiles obtained for these oils showed differences in composition among the studied species (Figure 1) although volatile constituents 76 CHIL. J. AGR. RES. - VOL. 71 - Nº 1 - 2011 % 1 α-Thujene 929 1.6 2 α-Pinene 937 3.1 2.9 3 Camphene 950 0.1 4 Sabinene 978 25.7 5 β-Pinene 983 2.8 6 β-Myrcene 990 59.0 0.8 0.2 7 α-Phellandrene 1002 1.0 8 α-Terpinene 1016 1.4 9 β-Phellandrene 1034 21.5 0.2 10 1,8-Cineole 1037 9.2 11 Z-β-Ocimene 1044 0.5 0.1 12 E-β-Ocimene 1053 0.7 13 γ-Terpinene 1065 2.5 14 Trans-4-thujanol 1072 6.3 15 α-Terpinolene 1091 0.5 16 Cis-4-thujan 1100 9.2 17 2-Cyclohenen-1-ol 1125 0.6 18 Terpinen-4-ol 1176 3.5 19 α-Tepineol 1191 3.5 20 Dihydroneoisocarveol 1229 1.2 21 Sabinene hydrate acetate (trans) 1254 0.4 22 Geraniol 1256 0.6 23 2-Undecanone 1294 1.7 24 δ-Elemene 1337 0.4 25 α-Cubebene 1359 0.3 0.1 26 α-Copaene 1375 0.5 0.4 27 Geranyl acetate 1387 0.4 28 β-Elemene 1398 1.6 0.9 29 E-Caryophyllene 1423 1.6 1.0 2.7 30 γ-Elemene 1433 0.2 31 α-Caryophyllene 1453 0.4 1.5 32 Alloaromadendrene 1459 0.5 33 γ-Muurolene 1472 0.4 34 Germacrene D 1479 9.3 2.3 6.0 35 Bicyclogermacrene 1488 3.1 1.5 5.8 36 Germacrene A 1495 1.4 0.4 37 γ-Cadinene 1510 1.8 38 δ-Cadinene 1520 0.9 4.1 39 Cadina-1.4-diene 1529 0.4 40 α-Cadinene 1533 0.4 41 Elemol 1547 1.9 8.4 42 Germacrene B 1552 0.3 0.8 43 Trans-1-nerolidol 1560 0.5 44 Germacrene D-4-ol 1573 1.2 21.1 45 Guaiol 1601 0.9 46 10-α-Eudesm-4-en-11-ol 1623 0.8 47 γ-Eudesmol 1631 1.3 48 Hinesol 1637 3.2 49 Tau-muurolol 1640 1.6 5.2 Table 1. Chemical composition of Zanthoxylum species oils. Z. monophyllum Z. fagara Area Peak Constituents RI1 Z. rhoifolium 77 have been previously reported in other species of the Zanthoxylum genus (Moura et al., 2006). The main constituents found in Z. rhoifolium fruit oil were β-myrcene (59.03%), β-phellandrene (21.47%), germacrene D (9.28%), and bicyclogermacrene (3.13%). Approximately 99.2% of Z. rhoifolium oil composition was characterized. The remaining unidentified components were mainly sesquiterpenes. The abundance of monoterpenoid and sesquiterpenoid compounds in fruit essential oil is in accordance with one previous report (Gonzaga et al., 2003); however, oil composition described in this study was qualitatively and quantitatively different, particularly regarding the major constituents. This suggests a considerable variability in the studied oil samples because of the influence of the ecological and chemical environment of each species, which affects the presence and abundance of secondary metabolites (Spitaler et al., 2006). The major constituents of oil derived from fruits collected in Brazil were reported as menth-2-en-1- ol (46.2%), β-myrcene (30.2%), (-)-linalool (15%), and terpineol (8.45%). The major constituents identified in Z. monophyllum fruit oil were sabinene (25.71%), 1,8-cineole (9.19%), trans-sabinene hydrate (9.19%), and cis-sabinene hydrate (6.25%). Zanthoxylum fagara fruit oil mainly contained germacrene D-4-ol (21.1%), elemol (8.35%), α-cadinol (8.22%), germacrene D (5.96%), bicyclogermacrene (5.75%), epi-α-muurolol (5.15%), and 5-neo-cedranol (5.12%). Approximately 89.94% of Z. monophyllum and 94.2% of Z. fagara oil compositions were characterized. The remaining unidentified components were monoterpenes and sesquiterpenes. The detailed composition of fruit essential oils of Z. fagara and Z. monophyllum are reported for the first time in this study. Continuation Table 1. % 50 Torreyol 1644 0.4 1.0 51 β-Eudesmol 1649 0.9 3.6 52 α-Cadinol 1654 4.1 8.2 53 5-Neo-cedranol 1680 5.1 54 Caryophyllene acetate 1701 0.6 55 E.E-Farnesol 1725 1.3 56 α-bisabolol acetate 1794 0.2 57 E.E-farnesyl acetate 1850 0.3 Monoterpenes --- 80.5 71.6 6.2 Sesquiterpenes --- 18.7 18.2 88.8 TOTAL --- 99.21 89.84 94.96 1Calculated retention index. Z. monophyllum Z. fagara Area Peak Constituents RI1 Z. rhoifolium Figure 1. Chemical profile of essential oils from Zanthoxylum species fruits. For the key to identify peaks, see Table 1. J. PRIETO et al. - CHEMICAL COMPOSITION, INSECTICIDAL, AND…….. 78 CHIL. J. AGR. RES. - VOL. 71 - Nº 1 - 2011 Insecticidal activity of the oils Results show that the essential oils of Z. fagara, Z. rhoifolium, and Z. monophyllum have different insecticidal activity against S. oryzae adults. Insecticidal activity rises by increasing the dose and exposure times (Figure 2). Zanthoxylum monophyllum essential oil showed the best fumigant activity against rice weevil, Z. rhoifolium oil had weak fumigant toxicity while Z. fagara essential oil was inactive (Figure 2). Zanthoxylum monophyllum essential oil caused significant mortality (about 90 to 99%) at 976 μL L-1 air dose after 24 h exposure. After 48 h of treatment, a 484 μL L-1 air dose is enough to cause 90% insect mortality. Fumigant activity of Z. monophyllum can be attributed to 1,8-cineole, terpinen-4-ol, and α-terpinene present in the essential oil; these compounds have shown 100% mortality on insects of the Sitophilus genus after 12 h exposure (Lee et al., 2004; Kordali et al., 2006). The commercial fumigants Phosphamin (100 μg L-1 air) and Nuvan 50 (50 μL L-1 air) showed 100% mortality