[精华]常用引物序列

[精华]常用引物序列 序列(5'-3') 日常备库引物 1492R GGTTACCTTGTTACGACTT 27F\8F AGAGTTTGATCCTGGCTCA 35S GACGCACAATCCCACTATCC 3'AD AGATGGTGCACGATGCACAG 3'AOX\AOX1rev GGCAAATGGCATTCTGACAT 3'BD TAAGAGTCACTTTAAAATTTGTATAC 5'AD\GAL4AD\P17110 TACCACTACAATGGATGATG 5'AOX\AOX1for GACTGGTTCCAATTGACAAGC 5'BD\GAL4-BD-Cfor TCATCGGAAGAGAGTAG 96gIII\M13-96 CCCTCATAGTTAGCGTAACG a-FACTOR\Alphafor TACTATTGCCAGCATTGCTGC BAC1 AACCATCTCGCAAATAAATA BAC2 ACGCACAGAATCTAGCGCTT BGH\pCDNA3.1R TAGAAGGCACAGTCGAGG CMV-24 TTAGGACAAGGCTGGTGG CMV-30 ATAACCCCGCCCCGTTG CGCAAATGGGCGGTAGGCGTG\ATGGGCGGTCMV-F\CMV-Profor AGGCGTG CMV-R TCGTTGGGCGGTCAGC DuetDOWN1 GATTATGCGGCCGTGTACAA DuetUP1 GATCTCGACGCTCTCCCT DuetUP2 TTGTACACGGCCGCATAATC EBVrev GTGGTTTGTCCAAACTCATC EGFP-Cfor AGCACCCAGTCCGCCCTGAGC EGFP-Nrev CGTCGCCGTCCAGCTC GAL1-Profor AACATTTTCGGTTTGTATTACTTC GLP1 TGTATCTTATGGTACTGTAACTG GLP2 CTTTATGTTTTTGGCGTCTTCCA GLP4 GCCCTTCTTAATGTTTTTG H1-F\H1 TCGCTATGTGTTCTGGGAAA HSVtk-pArev GTCTCCTTCCGTGTTTCAG IRES-R CCTCACATTGCCAAAAGACG ITS4 TCCTCCGCTTATTGATATGC ITS5 GGAAGTAAAAGTCGTAACAAGG M13-47 CGCCAGGGTTTTCCCAGTCACGAC M13F\TrxReverse\M13-20\M13F-40 TGTAAAACGACGGCCAGT M13R CAGGAAACAGCTATGACC malE GGTCGTCAGACTGTCGATGAAGCC MT-Profor CATCTCAGTGCAACTAAA mU6F2(mouse) TAGTATCCGACGCCGCCATC NL1 GCATATCAATAAGCGGAGGAAAAG NL4 GGTCCGTGTTTCAAGACGG NOSR-primer CGTCCAGCTCCATGTTGC P1 CCAGGCTTTACACTTTATGC p10-Profor CGGACCTTTAATTCAACCC P2 GCGATTAAGTTGGGTAACGC pBABE3' ACCCTAACTGACACACATTCC PBABE5' CTTTATCCAGCCCTCAC pBADfw CCATAGCATTTTTATCCATAAG pBADrv\pTrcHis-rv ATCTGTATCAGGCTGAAAATC PBV220F AAGAAGGGCAGCATTCAAAG pCDNA3.1F CTAGAGAACCCACTGCTTAC pCMV5F TTCCAAAATGTCGTAATAAC pCMV5R ATTATAGAGGACACCTAGTC PCMV-F TCTAAAAGCTGCGGAATTGT PCMV-R TCCAAACTCATCAATGTATC pcoldI-R\pCold-SUMO-R GGCAGGGATCTTAGATTCTG pCold-TF-F1 CCACTTTCAACGAGCTGATG pDBLeu-F GAATAAGTGCGACATCATCATC pDEST22-F TCGATGATGAAGATACCCCACC pDEST22-R CTCGACGTCTTACTTACTTAGC PDONR-F TCGCGTTAACGCTAGCATGGATCTC PDONR-R GTAACATCAGAGATTTTGAGACAC pDsRED-ex-C1-F TCCCACAACGAGGACTACAC PEF-F\EF-1aForward TCAAGCCTCAGACAGTGGTTC pEGFP-C-3' TATGGCTGATTATGATCAGT pEGFP-C-5’ CATGGTCCTGCTGGAGTTCGTG pEGFP-N-3’\EGFP-Nrev CGTCGCCGTCCAGCTCGACCAG pEGFP-N-5' TGGGAGGTCTATATAAGCAGAG pFastBac-fw GTCCGAAACCATGTCGTAC pFastBac-R TGGACAAACCACAACTAGAATG pFastBac-rev CCTCTACAAATGTGGTATGG pFlag-CMV-F GGTAGGCGTGTACGGTGG pFlag-CMV-R GCACTGGAGTGGCAACTT PGEX-F GGCAAGCCACGTTTGGTG PGEX-R GAGCTGCATGTGTCAGAGG PH-Profor AAATGATAACCATCTCGC pIRESrv GCCCTAGATGCATGCTCG PJET1.2-F CGACTCACTATAGGGAGAGCGGC PJET1.2-R AAGAACATCGATTTTCCATGGCAG pLNCX-F AGCTCGTTTAGTGAACCGTCAGATCG pLXSN-F CTTGAACCTCCTCGTTCGAC pLXSN-R GTTGCTGACTAATTGAGATG pMAL-C2X-F TGCGTACTGCGGTGATCAAC pMAL-C2X-R CTGCAAGGCGATTAAGTTGG pMAL-C5x-R TGTCCTACTCAGGAGAGCGTTCAC PPC86-F\pDEST22-F1998 TATAACGCGTTTGGAATCACT PPC86-R\pDBLeu-R GTAAATTTCTGGCAAGGTAGAC pQE30-F TGAGCGGATAACAATTTCAC pQE30-R GTTCTGAGGTCATTACTGG pSHUTTLE-CMV-R GTGGTATGGCTGATTATGATCAG pSilencer4.1-F\2.0rev AGGCGATTAAGTTGGGTA PTRC99C-F TTGCGCCGACATCATAAC PTRC99C-R\PBV220R CTGCGTTCTGATTTAATCTG pYes2.R\CYC1-Terminator GCGTGAATGTAAGCGTGAC RFP-Nrev GTTCACGGTGCCCTCC RV-M AGCGGATAACAATTTCACACAGGA RVP3 CTAGCAAAATAGGCTGTCCC RVP4 GACGATAGTCATGCCCCGCG S.tag GAACGCCAGCACATGGAC SeqL-A GCAGTTCCCTACTCTCGC SeqL-B CATCAGAGATTTTGAGACAC SP6 ATTTAGGTGACACTATAG SV40 GGAGGCTTTTTTGGAGGC SV40-pArev\pSG5rv GAAATTTGTGATGCTATTGC T3 ATTAACCCTCACTAAAGGGA T7 TAATACGACTCACTATAGG T7(17base) ACATCCACTTTGCCTTTCTC T7ter TGCTAGTTATTGCTCAGCGG U6-promoter(human) CCGTAACTTGAAAGTATTTCG v1.5 GGACTTTCCAAAATGTCG V5rev ATCGAGACCGAGGAGAGG WPRE-R CATAGCGTAAAAGGAGCAACA XL39\CMV-24 ATTAGGACAAGGCTGGTGGG ITS1 TCCGTAGGTGAACCTGCGG