人轮状病毒抗体(RV-2

人轮状病毒抗体(RV-2 編質歳來嬬? 1.劔瞳?來指拷嚥圓豚敵業?購狼方R峙葎0.990參貧。 2.答坪嚥答需哘蛍艶弌噐9%才11% 殊霞袈律? 1ng/L -20ng/L 隠贋訳周式嗤丼豚? 1.編質歳隠贋??2-8?。 2?嗤丼豚?6倖埖 Human Rotavirus antibody FOR RESEARCH USE ONLY Drug Names Generic Name?Human Rotavirus antibody(RV Ab) ELISA Kit. Purpose This kit allows for the determination of RV Ab concentrations in Human Feces and Related samples. Principle of the assay The kit assay Human RV Ab level in the sample?use Purified Human RV antigen to coat microtiter plate wells, make solid-phase antigen, then add RV Ab to wells, Combined RV Ab antigen which With HRP labeled, become antigen - antibody - enzyme- antigen complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RV Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve. Materials provided with the kit Materials provided with the kit48determinations96 determinationsStorageUser manual11Closure plate membrane22Sealed bags11Microelisa stripplate112-8? Standard?22.5ng/L0.5ml:1 bottle0.5ml:1 bottle2-8?Standard diluent1.5ml:1 bottle1.5ml:1 bottle2-8?HRP-Conjugate reagent3ml:1 bottle6ml:1 bottle2-8? Sample diluent3ml:1 bottle6ml:1 bottle2-8?Chromogen Solution A3ml:1 bottle6ml :1 bottle2-8?Chromogen Solution B3ml:1 bottle6ml:1 bottle2-8?Stop Solution3ml :1 bottle6ml:1 bottle2-8?wash solution20ml:1 bottle30ml:1 bottle2-8? Specimen requirements 1. serum- coagulation at room temperature 10-20 mins?centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it. 4. cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS? PH7.2-7.4?, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.