IGFⅡ,IGFⅠR在结肠腺瘤中的表达及其生物学意义

IGFⅡ,IGFⅠR在结肠腺瘤中的达及其生物学意义 DOC论文～方便的论制修改论您减 ??在论论腺瘤中的表IGF,,IGF,R 达学及其生物意论 ;作者论位论论,:___________: ___________: ___________ 作者,论论～ 范 论～ 沈琰～ 李新忠～ 论小平～ 袁永翀 【摘要】 目的, 论察IGF?,IGF?R在论论腺瘤中的表～探论其生达,, 物意论。, 免疫论化方法分论论论学IGF?,IGF?R,PCNA在正,, 常论论膜论;黏A论n=20,、低论论上皮瘤论论;内B论n=50,、高论论上皮内 瘤论论;C论n=50,和论论癌论;D论n=20,中的表～达TUNEL法论论论胞 凋亡。论果, IGF?,IGF?R,PCNA在论论癌论和高论论上皮瘤论论中内,, 呈高表～者之论无论著差达两异(P0.05)～分论低论论上皮瘤论论、正常与内 大论膜论相比表有论著差黏达异(P0.01)～低论论上皮瘤论论、高论论上皮内内 瘤论论、论论癌论的凋亡指数(apoptotic index,AI)逐论降低～且比论有论两两 著差异(P0.01)。IGF?,IGF?R的表增殖指;达与数proliferative ,, index,PI,成正相论、与AI成论相论～PI,AI在低论论上皮瘤论论内 IGF?,IGF?R论性和性中无论著差阳异(P0.05)～在高论论上皮瘤内,, 论论论有论著差异(P0.01)。论论, IGF?,IGF?R的常表论论腺瘤异达与,, 癌论密切相论～IGF?,IGF?R促论论胞常增殖～抑制论瘤论胞凋亡异～,, 在论论腺瘤癌论论程中起了重要的作用。 【论论论】 论素论生论因子胰,?; 论素论生论因子胰,?受体; 论论腺瘤 ,Abstract, Objective: To observe the expression of IGF?and , IGF?R in colorectal adenoma and investigate its biologial , significance. Methods, 140 patients were divided into four groups,20 cases of normal colon mucosa(A group)～50 cases of low grade colon intraepithelial neoplasia(B group)～50 cases of high grade colon intraepithelial neoplasia(C group) and 20 cases of colorectal carcinoma(D group). The expressions of IGF?,IGF?R,PCNA and apoptosis were detected by ,, immunohistochemical and TUNEL. Results, The expressions of IGF?, IGF?R and PCNA in C and D group was higher ,, than A and B group and there was no significant difference between C and D group(P0.05); compared with A and B group there were significant differences(P0.01); AI(Apoptotic index) in adenomas and adenocarcinomas of adenomacarcinoma sequence , was gradually less(P0.0l).The expressions of IGF?, IGF?R ,, had positive correlation with the expression of PI(proliferative DOC格式论文～方便的论制修改论您减 index) and negative correlations with the expression of AI. The expression of PI,AI in B group had no significant difference between the positive and negative expression of IGF?,IGF?R(P0.05),but in D group had significant ,, difference(P0.01). Conclusion, Abnormal expressions of IGF?,IGF?R have intently relations from colorectal adenoma ,, to carcinoma; IGF?and IGF?R can promote cell abnormal ,, proliferation and inhibit tumor cell apoptosis.And have an important effect on the process from colorectal adenoma to carcinoma. ,Key words, IGF?～ IGF?R～ colorectal adenoma,, 论论癌是常论的论性论瘤之一～是论腺瘤论段多基因改论、多论段论展的论程～论论癌的癌论论胞的增殖、分化、凋亡论控常密切相论。论素论生论与异胰 因子;insulinlike growth factors, IGF,论胞增殖、凋亡以及论瘤与, 的论生密切相论～IGF?,IGF?R作论IGF家族的重要成论～论论癌与,, 的相论性日益受到论注,1,。本论论通论免疫论化SP法论论 IGF?,IGF?R,增殖论胞核抗原;proliferating cell nuclear ,, antigen,PCNA,在论论腺瘤论论中的表～达TUNEL法论论论胞凋亡～论一步探论IGF?,IGF?R在论论腺瘤癌论中的作用。,, 1 论象方法与 1.1 究论象研 本论论收集了2004年1月,2007年3月我院及江论大附院手论学属医 切除或论论论活论的论论腺瘤论论存论档蜡100例～按照《全大论癌病理并国研 究论一论范》之论准论行腺瘤分论、不典型增生分论以及腺瘤癌论的论。论论中断 将两不典型增生分论大论:低论论上皮瘤论论内50例和高论论上皮瘤论论内50例～论另20例正常论论膜论论及黏20例论论腺癌膜论论作论论照。所有患者黏 均未行化论、放论及免疫治论。论论前行HE染色以论论论。断 1.2 方 法 所有论本论4 μm厚论论切片～免疫论化SP法论行染色～论微论论察论果。兔抗人IGF?多克隆抗、抗人体兔IGF?R多克隆抗论自博士德体,, 生物工程有限公司～抗人兔PCNA多克隆抗、体TUNEL论论盒论自博奥森生物工程有限公司。IGF?,IGF?R的染色论果判定考参,, Kawamato的论准,光论微论下棕色论胞学黄数30%论论性～31%,50%论论(+)～51%,80%论论(++)～?81%论论(+++)。论胞增殖指数(proliferative index,PI,以PCNA性论胞论判论果,平均每阳数断即100论胞中个 PCNA性论胞。阳数TUNEL法论论论胞凋亡～以凋亡指;数apoptotic index,AI,论判论果,平均每断即100论胞中凋亡论胞。个数 1.3 论论分析学 采用SPSS11.5论论论件论论论据论行论论论理～率的比论采用学数学χ2论论及Fisher切率论算法～以确概α=0.05论论著性论论水准。 2 论 果 2.1 各论中IGF?,IGF?R性表率及论胞增殖和凋亡指阳达数,, IGF?性表率阳达,IGF?R性表率和论胞增殖指;阳达数PI,在,, 正常论论膜论、论论腺瘤论、论论癌论中逐论增高～论论癌论和高论论上皮瘤论论比黏内 DOC格式论文～方便的论制修改论您减 论无论著差;异P0.05,～论论癌论和高论论上皮瘤论论分论正常论论膜论、内与黏 低论论上皮瘤论论比论有论著差;内异P0.01,～正常论论膜论、低论论上皮黏内 瘤论论比论有论著差;异P0.05,。论胞凋亡指;数AI,在低论论上皮瘤论论内、高论论上皮瘤论论、论论癌论中逐论降低～比论均有论著差;内两两异P0.01,。论表1。表1 各论中IGF?,IGF?R的性表率及论胞增殖和凋亡阳达,, 指数 2.2 IGF?,IGF?R的表达与PI的论系,, IGF?在论论腺瘤中的表达与PI呈正相论;r=0.747～P0.01,～, IGF?R在论论腺瘤中的表达与PI呈正相论;r=0.738～P0.01,～PI, 在低论论上皮瘤论论;内B论,IGF?,IGF?R性论性论之论无论著阳与,, 差异(P0.05)～在高论论上皮瘤论论;内C论,者之论有论著差两异 (P0.01)～论表2。 2.3 IGF?,IGF?R的表达与AI的论系,, IGF?在论论腺瘤中的表达与AI呈论相论;r=-0.655～P0.01,～, IGF?R在论论腺瘤中的表达与AI呈论相论;r=-0.661～P0.01,～AI, 在低论论上皮瘤论论内IGF?,IGF?R性论性无论著差阳与异,, (P0.05)～在高论论上皮瘤论论者之论有论著差内两异(P0.01)～论表3。表2 IGF?,IGF?R在论论腺瘤中的表达与PI的比论表3 ,, IGF?,IGF?R在论论腺瘤中的表达与AI的比论,, 3 论 论 论素论生论因子;胰IGF,主要论瘤的自分与泌和旁分泌生论论论有论～作论促有论分裂因子论论瘤的生论有促论作用～在多论论瘤论论中论度表～促论论达瘤论胞的形成增殖～论性论瘤的论生、论展有密切的论与与研系。目前究论论IGF?促论论胞增殖生论的活性主要由IGF?R介论～IGF?R的,,,表上论论论癌的论生论展论达与系密切,1,。郭宝文等,2,究论论研IGF?,IGF?R表大论癌的论生、论展和达与研侵论论移密切相论。本论论,, 究论果论示IGF?,IGF?R的性表率在正常论论膜论、低论论上皮阳达黏,, 内内内瘤论论、高论论上皮瘤论论、论论癌论中逐论增高～在论论癌论和高论论上皮瘤论论呈高表～者比论无论著差～达两异两与黏但者分论正常大论膜论、低论论上皮瘤论论比论有论著差。内异提示论论腺瘤癌论论程中IGF?,IGF?R,,出论论度表。达 IGF?,IGF?R的主要功能论促论论胞增殖和分化。增殖论胞核抗原,, ;PCNA,论瘤的论生、论展、与估浸论、论移密切相论～已作论一论论论胞增殖状广研论的指论泛用于论性论瘤的究,3,。Kawamoto等采用免疫论化法论论92例大论癌论本,4,～论论论瘤论论中IGF?高表～且达并与PCNA, 表呈正相论。本究论果论达研示～PI在正常论论膜论、低论论上皮瘤论论、高黏内 论论上皮瘤论论及大论癌论中逐论增高。内异提示腺瘤论论的论胞常增殖促论了大论癌的论生～高论论上皮瘤论论腺瘤上皮内与已具有论性论胞十分相似的异研常论胞增殖能力。本究论果论表明,IGF?,IGF?R在腺瘤中的,, 表达与PI均呈正相论～PI在低论论上皮瘤论论内IGF?,IGF?R论性,,及性论中均论低增殖指～者之论无论著差～在高论论上皮瘤论论阳数两异内 均论高增殖指～且性论论性论的表有论著差。数阳与达异提示IGF?,IGF?R可能通论促论论胞常增殖促论了论论癌的论生。异,, 论瘤的论生论胞凋亡的与乱紊有论～IGF及其受是抗凋亡体号信的主要 DOC格式论文～方便的论制修改论您减 论论者,5,。Wu等,6,论论IGF?R论度表达可以阻止论瘤论胞凋亡～, 并指出IGF?R阻止论瘤论胞凋亡的作用是通论作用于其受～体最论论, 致位于论胞的受活性内体氨部位酪酸激论的活化。论论武等,7,采用免疫论化法论论人大论癌论胞株HT29中IGF?R及其抗的表论论体达～,, IGF?R McAb通论阻断IGF?R抑制论论癌论胞增殖、论论论胞凋亡。,, 本究论研随与示着腺瘤向腺癌论化～凋亡论胞比例逐论下降～论一论果Bedi等,8,及论保存等,9,论道的基本一致～IGF?,IGF?R的,, 表均达与AI呈论相论～AI在低论论上皮瘤论论内IGF?,IGF?R性阳,, 与异内论性之论无论著差～在高论论上皮瘤论论IGF?,IGF?R性论阳与,, 性之论论有论著差 。论果异提示IGF?,IGF?R可能通论抑制论瘤论胞,, 凋亡促论了论论癌的论生。 论上所述～IGF?,IGF?R的常表论论腺瘤癌论密切相论～异达与,, IGF?,IGF?R可能通论促论论胞常增殖、抑制论瘤论胞凋亡～异而论,, 致论论腺瘤癌论的论生～IGF?,IGF?R作论论论腺瘤癌论的论论指论～论得,, 论床论一步探论。 【考文】参献 ,1, 论文利.大论癌中论素论生论因子的究论展,胰研J,.论医学述～2004～10(5),259-260. ,2, 郭宝吴文～敏论～论 琳～等.IGF?和IGF?R在大论,, 癌中的表,达J,.山论论医,2005,45(10),6-8. ,3, 肖秀英.PCNA在论性论瘤中的究论展,研J,. 论家口医学院学论～2003～20(6),42-44. ,4, Kazuyki K,Onodera H,Kondo S,et al.Expression of insulnlike growth factor2 can predict the prognsis of hunan ,, colorectal cancer patients:correlation with tumor progression,proliferative activity and survival ,J,.Oncology,1998,55(1):242-248. ,5, 牛朝论. IGF?R论胞增殖、凋亡和论瘤,与J,. 国医学外,, 生理、病理科学与册论床分～2000～20;3,,212-215. ,6, Wu X,Fan Z,Masui H,et al.Apoptosis induced by an antiepidermal growth factor receptor monoclonal antibody in a , human colorectal carcinoma cell line and its delay by insulin ,J,.J Clin Invest～1995～95(4):1897-1905. ,7, 论论武～论有成～论小明～等.论素生论因子?型受论克隆抗胰体 体响论论论癌论胞生论的影,J,.中国普外科论志～2006～21(3):200-202. ,8, Bedi A,Pasricha PJ,Akhtar AJ,et al.Inhibition of DOC格式论文～方便的论制修改论您减 apoptosis during development of colorectal cancer ,J,.Cancer Res,1995,55(9):1811-1816. ,9, 论保存～论秀论～惠 京～等.大论癌及其癌前病论中论胞凋亡与论胞增殖论系的原位论察 ,J,.中论论医学志～1996,76(4):848.