产妇论文：产妇 新生儿 胰岛素样生长因子2 甲基化 出生特征

产妇论文：产妇 新生儿 胰岛素样生长因子2 甲基化 出生特征 产妇论文:产妇 新生儿 胰岛素样生长因子2 甲基化 出生特征 【中文摘要】探讨“孕期营养及环境因素-IGF2 DNA甲基化模式-新生儿出生特征”三者之间的关系,为肿瘤的预防及早期营养干预提供重要线索。方法:1.研究对象的选择:选择2008年4月至2008年9月在郑州市某乡中心卫生院住院的健康产妇作为调查对象,排除具有乙肝、糖尿病及其它消化道慢性疾病史的妇女,告知研究和意义后,共计99名产妇参加研究。2.血清中一碳营养物和血清锌的测定:化学发光免疫荧光法测定血清中叶酸、维生素B12;火焰原子吸收分光光度法测定血清锌。3.IGF2 DNA甲基化水平的测定:实时荧光定量(Real Time) PCR分析IGF2基因甲基化水平。4.新生儿出生特征的测量:电子秤称量新生儿体重;头围尺、卧式身长计分别测量新生儿头围和身高。5.数据整理分析与统计学处理:采用SPSS12.0软件。主要方法有:配对样本的t检验、多重线性回归分析等,以α=0.05为检验水准。结果:1.新生儿出生特征与母体血清营养物含量及母亲孕期环境因素的关系:(1)产妇及新生儿血清叶酸平均水平分别为2.29ng/ml和7.29ng/ml,维生素B12平均水平为175.34pg/ml和240.19pg/ml,锌平均水平为0.67μg/ml和0.93μg/ml。新生儿血清叶酸水平高于产妇叶酸水平(t=-12.183,P=0.001),且两者具有相关性(r=0.367,P=0.001);新生儿血清维生素B12高于产妇(t=-4.432,P=0.001),两者具有相关性(r=0.325,P=0.001);新生儿血清锌高于产妇(t=--5.551,P=0.001),但两者间无相关性。(2)新生儿 平均身高为49.48cm,平均体重为3.19kg,平均头围为33.83cm;新生儿出生体重、身长及头围分别与产妇孕前BMI相关(β =0.237,P=0.020;β=0.373,P=0.001;β=0.251,P=0.015)。(3)新生儿出生体重、出生头围分别与新生儿性别相关(β=-0.292,P=0.004;β=-0.217,P=0.036)。(4)新生儿身长与产妇剖宫产(β =0.210,P=0.034)相关。2.产妇及新生儿血清营养物含量及母亲孕期环境因素与IGF2 DNA甲基化模式的关系:(1)母血IGF2 P2甲基化率均值为2.84%,脐血为3.94%;母血IGF2 P3甲基化率均值为5.83%,脐血为4.11%。新生儿IGF2 P2的DNA甲基化水平高于产妇(t=-2.724,P=0.008),但两者之间无相关性;产妇IGF2 P3的DNA甲基化水平高于新生儿(t=4.376,P=0.001),且两者间具有高度相关性(β=0.403,P=0.001)。(2)新生儿IGF2 P2的DNA甲基化状态与产妇剖宫产具有相关性(β=0.287,P=0.006);新生儿IGF2 P3的DNA甲基化状态与产妇血清维生素B12(β=-0.279,P=0.004)、母亲受教育程度(β=0.213,P=0.030)分别相关。(3)产妇IGF2 P2的甲基化状态与产妇血清维生素B12(β=-0.231,P=0.020)、孕期体重增加(β=0.229,P=0.023)和被动吸烟(β=0.231,P=0.020)分别相关。产妇IGF2 P3的甲基化状态与产妇血清锌(β=-0.234,P=0.022)和产妇受教育程度高(β=-0.290,P=0.005)分别相关。3.产妇及新生儿IGF2 DNA甲基化状态与新生儿出生特征无相关性;结论:1.产妇孕前BMI及剖宫产可影响新生儿的出生体重、身长和头围。2.母血及脐血IGF2甲基化的水平可能受母血维生素B12水平及孕期被动吸烟影响,且这 种影响具有启动子区特异性。3.未发现新生儿的出生体重、身长和头 围与产妇及新生儿IGF2甲基化状态有关。 【英文摘要】:The aim of this study was to explore the relationship among “maternal nutritional intake and environmental factors-IGF2 DNA methylation patterns in offsprings-birth characteristics”, and to provide important clues for cancer prevention and nutritional intervention.Methods:1. Selection of subjectsPregnant women, without medical conditions such as diabetes, hepatitis infection or other coeliac diseases, and came to the hospital for delivery between April and September 2008, were considered eligible for the study. The total of 99 mothers participated in this study after being informed the purpose and significance of this study.2. Determination of one-carbon nutrients in serumSerum folate and vitamin B12 were determined using paramagnetic particle chemiluminescent immunoassay method. Serum zinc was determined using Atomic absorption spectrophotometry.3. The determination of IGF2 DNA methylation statusMethylation patterns of IGF2 were analyzed by Real time-PCR.4. Measurement of birth characteristicsThe body weight of offspring was weighed by electronic scale. The head circumference and the birth length of offspring were measured using horizontal length meter.5. Data management and statistical analysisAll analyses were performed using the SPSS 12.0. Major methods included paired sample T-test, multiple linear regression analysis and so on. A P-value of less than 0.05 was considered statistically significant.Results:1. The association between birth characteristics with nutrient content of maternal serum and maternal environmental exposures during pregnancy.(1) The mean level of serum folate was 2.29ng/ml for maternal and 7.29ng/ml for offsprings respectively. The mean level of serum vitamin B12 was 175.34pg/ml for maternal and 240.19pg/ml for offsprings respectively. The mean level of serum zinc was 0.67μg/ml for maternal and 0.93μg/ml for offsprings respectively. Serum folate levels (t=-12.183,P=0.001), vitamin B,2 levels (t=-4.432,P=0.001)and zinc levels (t=-5.551,P=0.001) in cord blood were significantly higher than in mother’s blood respectively. Serum folate levels (r=0.367, P=0.001) and vitamin B12 levels (r=0.325, P=0.001) in cord blood were correlated with mother’s blood respectively. Similar correlation, however, was not observed for zinc.(2) The average birth length of offspring was 49.48cm, the average weight was 3.19kg and the average head circumference was 33.83cm. Birth weight, length and head circumference of offsprings was related with pre-pregnancy body mass index of maternal respectively (β =0.237, P=0.020;β=0.373,P=0.001;β=0.251, P=0.015).(3) Birth weight and head circumference of offsprings were related with sex of newborn respectively (β=-0.292, P=0.004;β=-0.217, P=0.036).(4) Birth length of offsprings was related with maternal cesarean (β=0.210, P=0.034).2. The association between serum nutrient content of maternal and offspring and maternal environmental exposures during pregnancy with IGF2 DNA methylation pattern.(1) The mean percentages of methylation in P2 promoter were 2.84% for mother’s blood and 3.94% for cord blood respectively. The percentages of methylation in P3 were 5.83% for mother’s blood and 4.11% for cord blood respectively. The percentage of methylation in P2 in cord blood was significantly higher than in mother’s blood (t=-2.724, P=0.008), but uncorrelated with each other. The percentage of methylation in P3 in mother’s blood was significantly higher than that in cord blood (t=4.376, P=0.001), and significantly correlated with each other (β=0.403, P=0.001).(2) IGF2 P2 DNA methylation status of offsprings was related with maternal cesarean (β=0.287, P=0.006). IGF2 P3 DNA methylation status of offsprings was related with maternal serum vitamin B12 (β=-0.279, P=0.004) and education level of mothers (β=0.213,P=0.030).(3) IGF2 P2 DNA methylation status of maternal was related with maternal serum vitamin B12 (β =-0.231, P=0.020), weight gain during pregnancy (β =0.229,P=0.023) and passive smoking (β=0.231, P=0.020). IGF2 P3 DNA methylation status of maternal was related with maternal serum zinc (β=-0.234,P=0.022) and education level of mothers (β=-0.290,P=0.005).3. The significant correlation between IGF2 DNA methylation pattern of maternal and offsprings was not found.Conclusions:1. Maternal pre-pregnancy body mass index and cesarean may be associated with birth characteristics of offsprings.2. Exposure to passive smoking during pregnancy and maternal serum vitamin B12 may be associated with IGF2 methylation status of maternal and offsprings.3. IGF2 methylation status of maternal and offsprings may not be associated with birth characteristics of offsprings. 【关键词】产妇 新生儿 胰岛素样生长因子2 甲基化 出生特征 【英文关键词】Maternal Offsprings Insulin-like growth factor 2 Methylation Birth characteristics 【目录】孕期营养及环境因素对胎儿IGF2 DNA甲基化模式的影 响 摘要 4-6 Abstract 6-8 1 引言 13-15 2 对 象与方法 15-22 2.1 调查对象的选择 15 2.2 血清叶酸的检测 15 2.3 血清维生素B_(12)的检测 15 2.4 血清锌含量的测定 15-16 2.5 新生儿出生体重、头围和身长的测量 16 2.6 基因组DNA的提取及IGF2基因甲基化分析 16-20 2.6.1 实验主要材料 16 2.6.2 实验主要仪器 16 2.6.3 试验方法 16-20 2.7 质量控制 20 2.8 数据整理分析与统计学处理 20-22 3 结果 22-33 3.1 产妇及新生儿的基本情况 22-24 3.2 产妇与新生儿血清叶酸水平的比较 24 3.3 产妇与新生儿血清维生素B_(12)水平的比较 24 3.4 产妇与新生儿血清锌水平的比较 24 3.5 服药与未服药产妇血清叶酸、维生素B_(12)和锌水平比较 24-25 3.6 产妇与新生儿血清叶酸、维生素B_(12)、锌的相关性分析 25 3.7 产妇与新生儿IGF2 DNA甲基化水平的比较 25-27 3.8 新生儿出生特征与母体血清营养物含量及母亲孕期环境因素的关系 27-29 3.9 产妇及新生儿血清营养物含量及母亲孕期环境因素与IGF2 DNA甲基化模式的关系 29-31 3.10 产妇及新生儿IGF2 DNA甲基化状态与新生儿出生特征的关系 31-33 4 讨论 33-39 4.1 叶酸、维生素B_(12)、锌及孕期环境因素与新生儿出生特征的关系 33-35 4.1.1 产妇和新生儿血清中叶酸、维生素B_(12)及锌的关系 33-34 4.1.2 叶酸、维生素B_(12)、锌与新生儿出生特征的关系 34-35 4.1.3 产妇孕期环境因素与新生儿出生特 征的关系 35 4.2 叶酸、维生素B_(12)、锌及孕期环境因素与IGF2 DNA甲基化的关系 35-37 4.3 产妇及新生儿IGF2 DNA甲基化模式与新生儿出生特征的关系 37-39 5 结论 39-40 参考文献 40-44 附录A 44-55 综述 叶酸、DNA甲基化与胎源性疾病关系的研究进展 55-66 参考文献 63-66 个人简历 66 在学期间发表的学术论文及研究成果 66-67 致谢 67 【索购全文找】1.3.9.9.3.8.8.4.8 1.3.8.1.1.3.7.2.1 同时提供论文写作一对一辅导和论文发表服务。 【说明】本文仅为中国学术文献总库合作提供，无涉版权。作者如有异议请与总库或学校联系。