龙牡壮骨冲剂对胃肠动力及胃肠组织VIP的影响
龙牡壮骨冲剂对胃肠动力及胃肠组织VIP
的影响
VIP??????
???L???????????????Y????
???6?V?????????????(430070
????:[????]?l?????????????????????????B???5?g?????????????????g??????mI[????]?0?5?g???v?0?v??????mV?M????mV??
?????????B?d?B????mV?E?B????mV?5?B????,?r???)??mV?k????mV???????<mV???]???g???4???y???[???4?)?b?????????]???5?g?M
??,???G???d?%??????????,?7???????#?????????????5????????,?(?????y?????#?????????E?N???T?d????(VIP)?Y??mI[??
??]???)???????????????L,?(???????????????EVIP?Y??????(?/?vP<005)mI?k???????????????????????????????EVIP
?Y???/??(?/?vP<005)mI???????<?????5?????????????????????????EVIP?Y??????(?/?vP<005)mI???]???g?????5??
?????????????????????????EVIP?Y???/??(?/?vP<005)mI???????????B?????W???5?g???????????5??????????????,?d
?B?????????????B?????)???N???????????LmV?k?????N????????????mV???????<?N???5????????????mV???]???g?N???5??????
???????[?0?????r(?/?vP<005),?E?5?B?????????????B???[???????0?????rmI???????????B???4???N?????????gVIP?]
???[?0?????r,?A?<?B???[???????????dmI[????]?????????????????R?P??????????,?????[?B???????d,???????????????P
?????r?????p?l???????N???????E??VIP?Y????????mI
???x??:????????;???????????B;?????N???T?d????;???????g;?5?g
?E???0????:R2855???x?7???V:A?????9??:10055509(2010)05065203
InfluenceofLongMuZhuangGuPowderonGastrointestinalFunctioninExperimentAnimalsHuangJuan,XuHui,LuoYanjuan,et
alHubeiMaternalandChildrenHospital,Wuchang(430070)
Abstract:[Objective]ToobservetheinfluenceofLongmuzhuanggupowder(LMZGP)ongastroentericmovementandguthormone
inmice.[Methods]Toestablishthemouseofthedisorderofgastricdepletionandsmallintestimeadvancementbyusingmaxolon,
atropine,neostigmineandadrenaline.Micewererandomlydividedintocontrolgroup,modelgroup,LMZGPlargedosegroup,
LMZGPmiddledosegroup,LMZGPlowdosegroup.Gastricemptyingandintestinalprogradatingratesinmodelmiceweretested
withblacknutritiousemisolidcataplasm.Theexpressionofvasointestinalpolypeptide(VIP)wastested
byradioimmunoassay.
[Results]MaxolonacceleratedgastricdepletionanddegradedthelevelofVIPingastroenterictissue.Atropineinhibitedgastric
emptyandheightenedthelevelofVIPingastroenterictissue.Neostigmineacceleratedintestinepropagationanddegradedthelevel
ofVIPingastroenterictissue.AdrenalineinhibitedintestinepropagationandheightenedthelevelofVIPingastroenterictissue.
LMZGPadministratedorallyhadnosignificantinfluenceonthegastricdepletionandsmallintestineadvancementinnormalmice,
butimprovedthegastroentericmovementinanimalmodelofgastroentericmovementdisorderswhichmanifestedasrelievinggas
tricdepletiondisorderbyatropineandmaxolon,antagonizingsignificantlytheaccelerationorloweringofsmallintestineadvance
mentinducedbyneostigmineandadrenaline.MiddleandlowdoseofLMZGPalsohadsomereverseeffect.LMZGPalsodosede
pendentlyantagonizeddisordersofVIP.[Conclusion]LMZGPhasdualdirectionalregulatingeffectonintestinepropagationviaa
mechanismofactionmainlyonVIPingastroenterictissue.
Keywords:gastroentericmovement;Longmuzhuanggupowder;vasointestinalpolypeptide(VIP);gastroenterichormone;mouse
???????????6?V???????~??????(N63B63)
FjIySB(N63B63)
?5?k?????????d?????K?0?W??,?E?T?A??????
?5?k?????????d?????W???y??????,?????0?????v
?y?W?)??mV???A?W??mV?l?X??,???8??mI????
???????B?p???6?V?E?;???d????,?T???r????????
???J?5?k??????,??????;???5?k?l??mV?e??mV???J?)
?pmV???9?)??mV???????8?!?????[???????rmI?F?A??
?????H?r??????????????????????????????????,
???s?????????????????M?????s?rmI
1?????<????
11???????d?0?<???5?g,???/18~20g(4~6
??),???????r,?E?????6?V?T????????????????
?E??mI
65
?????E?T???d??????2010??5?:?F34???F5?L
:o:200AA011
undproect:temsupportedbHubeiProvincialci-techureauo:200AA011
2
,?!???????????B????????????????????VI??????
12???????B?y?????#VIP,?y?????B???E???6
???????L?????p?A???NmI???????????B?v???!???R
???l?????L??;???)???v???????????l?[???????L
??;?k?????v?]???m???b???[???????L??;???????<
?v???e?s???b?????L??;???]???g?v???!???d?b??
?????????[???????L??mI
13????OHAUSPT200S?j???s??,????????;
Labofug400R?????v,????Heraeus????????mI
2????????
[1]
21?G???d?%???????b??
[2]
??5g???J???????g??
??25ml?????Y?E,?b?<?0?i????8g????mV4g??mV4g
????mV3g?T?d??,?3???/??,???;150ml??150g???%
??????,???????E????12h,???r??2h???????)
?W?smI
22???????????B?????W?5?g?????????5??????
???????0?<???5?g40??,?[???????v?0?;4??,?v
??????(?????Y????)mV???????????B?dmV?EmV?5?B??
??(?????B???0?i?v63g/kgmV32g/kgmV16g/kg,??
??),????75min?<?????G???d?%??????1ml/??,
30min?<?H???4??????,????????,???????J??????
??mI?????r?????????<??????,?b?<?????d?p????
????,?6???????????<?r????????,????????,????
?????????????z?v????????????,???#?<?N??????
?????0???v????????????????????,??????????=
(????-????)/????!100%mI???????4???]???5?g
????,?)?????1???????????],?0?i??????????????
????????????????????????????mI?????5????????
(%)=(????????????/??-??????)!100%mI
23???????????B?????)???N???5?g??????????
???0?<???5?g40??,?[???????v?0?;4??,?v?M??
??mV???????????B?dmV?EmV?5?B????(?B???(?],??
??),?M?????5?g?????Y????,?<3???5?g????????
?B?????????????B,60min?<?4???5?g?/???????)??
5mg/kg?y???[???4mI15min?<?????G???d?%????
??1ml/??,?(22,??????????????mI
24???????????B???k?????N??????????????
?0?<???5?g40??,?[???????v?0?;4??,?v?M????mV
???????????B?dmV?EmV?5?B????(?B???(?],????),?M
?????5?g?????Y????,?<3???5?g?????????B????
?????????B,60min?<?4???5?g?/?????k????15mg/
kg?y???[???4mI15min?<?????G???d?%??????
1ml/??,?(22,??????????????mI
25???????????B?????????<?N???5?g?5??????
???????0?<???5?g5??,?[???????v?0?;5??,?v
?M????mV???????????B?dmV?EmV?5?B????(?B???(?],
????),?M?????5?g?????Y????,?<3???5?g??????
???B?????????????B,60min?<?4???5?g?/????????
???<008mg/kg?y???[???4mI15min?<?????G???d
?%??????1ml/??,?(22,?????5????????(%)mI
26???????????B?????]???g?L???5??????????
???0?<???5?g40??,?[???????v?0?;4??,?v?M??
??mV???????????B?dmV?EmV?5?B????(?B???(?],??
??),?M?????5?g?????Y????,?<3???5?g????????
?B?????????????B,60min?<?4???5?g?/???????]??
?g04mg/kg?y???[???4mI15min?<?????G???d?%
??????1ml/??,?(22,?????5????????(%)mI
27???????????????5?g?????????N???T?d????
(VIP)??????:?5?g?4??mV????,????????????????,
?6?????????????<??????,??1ml100Z??L?0???Y?E
????3min,????1N??????05ml?E?????U???E??
?U????,????1N??NaOH05ml?E??,4Z??????,,
????(3500r/min)30min,???]?3??,-20Z??,????mI
?F???????r?y?4?????0???????????????#??mI
28???????4?0?????r?/???????7???????v(x
#sd),???????????r?????0??,?HSPSS130??????
???4?0mI
3????
31???????????????5?g????????????????1mI
??1???????????????5?g??????????????(x#s,%)
???in???W?5?g???)?????k??????
??????10492#81492#81492#81
?M????10ZP323#90
a
267#53
a
?d?B????10458#86484#72
b
484#142
b
?E?B????10401#128408#66
b
427#103
b
?5?B????10447#153365#49353#95
ab
?<????????????,a
P<005;?<?(?6?M????????,b
P<005
32???????????????5?g?5??????????????
??2mI
??2???????????????5?g?5????????????(x#s,%)
???in???W?5?g???????<?????]???g??
??????10658#93658#93658#93
?M????10ZP797#68
a
466#86
a
?d?B????10683#103684#113
b
592#80
b
?E?B????10665#84692#131578#74
b
?5?B????10633#85732#75
a
547#58
a
?<???W????????,a
P<005;?<?(?6?M????????,b
P<005
33???????????????5?g????????VIP??????
????3mI
????
?E?T???v?5?k?L???????)???l?<?y?????????7
653
?????E?T???d??????2010??5?:?F34???F5?L
:P
04
,?!???????????B????????????????????VI??????
??3???????????????5?g????????VIP??????(x#s,pg/100mg)
n???W?5?g???)?????k?????????????<?????]???g??
??????101052#2161052#2161052#2161052#2161052#216
?M????10ZP593#126
a
1721#189
a
737#145
a
1648#311
a
?d?B????101497#152
a
987#162
b
1179#229
b
1100#118
b
994#207
b
?E?B????101132#293863#144
b
1294#256
b
943#173
b
1245#136
ab
?5?B????101134#183735#150
ab
1473#174
ab
826#166
a
1378#284
a
?<???W????????,a
P<005;?<?(?6?M????????,b
P<005
????mI?Y???d???A???????????????????B???[?y??
?????????rmI???????????B?i????mV????mV????mV??
??(???b)mV???p(??)mV????mV??????(???b)mV?????!15
???E??,?N?i????mV?????????v???W?????????o????
??,?v???????????o??,????????NK????????,??
???????????r?????????T,???????????;?????[??
?R?P?????r,?r???y?A??;???p?????E?w,???????y
???/??,???????y?????L???#??,?r?????????]????
?????=?E???r????;????mV?p??mV?d??mV?n?!???A??
?y,???<?s?L?9?#??;????mV?????????n?9?Y,?????L
?G;?M?????????9?????]?????????(?)??,????????
???9?W?b????????mI????????????????mV???????ymV
???A???YmV?????????#??,???????????B???????[??
?ymV?????!???=mI????,???#???????????B???????5
?k????????mI
?F???????????x
[3]
,?r???)??mV?k????mV??????
?<mV???]???g???4???y???[???4?)?b?????????]??
?5?g?M??mI?????y?v???)???????????????L,?k??
????????????????,???????<?????5????????????,
???]???g?????5????????????,???v?g?M?;??mI?F
???????????????????5?????????v?7?7,???r?G??
?d?%???????v????,???G???????????????????W??
??,????????????mV?????m???g??????????????????
????????
[2]
mI
?F?A??????,???????????B?????W?5?g??????
???5??????????????,?U?????)???N???????????LmV
?k?????N????????????;???????<?N???5????????
????mV???]???g?N???5?????????????[?0?????r,?A
?????=?????,???B?????d???r?<?ymI???<?F???r??
???????????9?????v?????V?,???[???R?P?????r,
?w?:?????E?T?0???????????m?O????
[4]
,?????E
???)?????r???l????,?[?i???????????b?B,???r??
???????v?b,?????m???R?P?????r,???????E?????0
???r???n??mI
?????????0?????????????g?9,???[????????
?????<???????????P??mI?E?T???v?y???f?H????,
???????A?????????????g?????l?9?v?R?????1????
???????H?W,?a?3?r?Y??????mV?????????v???d???9
???9???d???9?????????0?L?0?O???[???l??
?(??
[5]
mI
VIP???0?????????????r????????(????????
????),?T???????r?????????0?????????????,????
??????????mV?????????umI???[???????????u???2
?N?????r,???b?????0??,????HCO
-
3???0??,?v??
?????5?????????L
[6]
mI?????[?A?????vVIP?p????
???????????????#?????????r???????????0??mI?r
???V???????????dmV???v???????????a???J??????
?????????????????a?????)?E???N???x???r
[7]
mI
?F???????????A??????:???)??????????????
?L???(???????????????EVIP?Y??????;?k??????
???????????????(???????????????EVIP?Y???/
??;???????<?????5???????????????(??????????
?????EVIP?Y??????;???]???g?????5??????????
???Y???????????EVIP?Y???/??mI???????????B??
?4???N???????????N??VIP?Y?????O?????[?0????
?r,?A?<?B???[???????????dmI?-?0????????????
???B?P??????????,???v?b?#???????l???P???5?g
VIP???:?;?????y,?P?????????????b?s,???g?N
??,?O?????????????N?????K??????mI
???P???x:
[1]??????,?g?!?/.?????????????d?????d???9?)???d?g
???????????????g??????[J].???e?E?T???d??????,
2005,29(2):156158.
[2]??????.???????B???5?g???????????????v?b?????A??
[J].?E???E?T???N?T???B??,2004,10(6):4243.
[3]?=?/.?E?????0?A????????[M].?6??:???R???L???=
?y,2006:438442.
[4]?????3.?3???9?????????????9?)???v?T????????????
?????3?N?3???????g????????????????[J].?E???E?T
???N?T???B??,2004,10(1):6366.
[5]????.???I???u???????y???d?g?????????????????g??
????[J].?????T????????,2006,29:100102.
[6]???a??.???????:?B???d?g?????????????r?????v?b??
??[J].?????E?T?B??,2005,32(12):13271329.
[7]?g?!??.?????????????????????N?U?T?d???????9???A
??[]?E???T???d??????,6,35()3
(?b?k???L)
65
?????E?T???d??????2010??5?:?F34???F5?L
:P
J.2002:18184.
20091109
4