针刺及磁提针对骨骼肌静息电位恢复的作用

针刺及磁提针对骨骼肌静息电位恢复的作用 针刺及磁提针对骨骼肌静息电位恢复的作 用 174 中国临床康复第9卷第42船2005—11-14出版 ChineseJoumdofClinicalRehabilitation,November142005Vo1.9No.42 ? TRADlTloNALCHlNEsEMEDlClNEFORREHABlLlTATloN? Effectofacupunctureandmagneticliftingneedleontherecoveryof restingpotentialofskeletalmuscle? xuYu?ming,ZhouXin.YinXiao?hui PhysicalEducationCollege,ShanxiNormalUniversity,Linfen041000, ShanxiProvince.China;~handongProvincialBinzhouCollege.BinzhOH 256604,ShandongProvince,China XUYU?ruing?.Studyingfordoctorate.Associateprofessor.PhysicalEdu. cationCollege.ShanxiNormalUniversity,Linfen041000.Shanxi Province.Chinaxuyumingl10@126.com Received:2005—oo—o0Accepted:2005—)o0fOl/SS1 Abstract BACKGRoUND:Thelong-termcontractionofskeletalmusclecanlcad tothedecreaseofrestingpotentia1.affectitscontraction,andresultinthe occurrenceoffatigue.Whetheracupunctureandinjury-freemagneticlifting needleareeffectiveontherecoveryofrestingpotential? oBJECTIV~-”Toobserveandcomparetheinflueneeontherecoveryof restingpotentialofskeletalmusclebytakingacupunctureandmagnetic needleastherecoverymeans. DESIGN:Acontrolledstudy. SrrDiGS:CollegeofPhysicalEducation.ShanxiNormalUniversity; BinzhouCollegeofShandongProvince. M_ATERLLS:Thirtyexperimentaltoads.whichwereprovidedbyBering UniversityofPhysicalEducation.wererandomlydividedintoacupuncture group(n=lo】,magneticneedlegroup(n:10),magneticneedle+pressing group(n=1O).Leftandrightsemitendinosusmusclesweretakenascon? trois. M匝_THoDS:Thenvitrotoad’ssemitendinosusmusclesfexperimental sideandcontrolside)weretreatedwithelectrostimulationfor40minutes. andthentherestingpotentialofskeletalmuscularcellsweremeasured withtheintracellularassmieroelectrodetechnique.Aftertheelectrostim. ulation,ftliformneedleacupuncture,magneticneedle,magneticneedie pluspressingwerealsoven. MLf6NoUTCoMEM哐ASURES:Therestingpotentialswereobserved beforeexercise.afterelectrostimulationandothertreatmentsinthegroups. RESULTS:?Therestingpotentialsofskeletalmuscularcellsafterelec? trostimulationweredecreasedascomparedwiththosebe~reelectrostimu. 1ation【experimentalside:(59.48?7.37),(84.54?5.72)mV;controlside: (59.43?7.14】,(83.62~5.86)mY].?Afterthetreatmentsofacupuncture, magneticliftingneedle,magneticliftingneedlepluspressing,thede— creasedrestingpotentialsafterelectrostimulationwereobviouslyrecovered (P<0.o1).?Therecoveredpercentageofrestingpotentialinthemagnet? icBeedlegroupwasobviouslysmallerthanthoseintheacupuncturegroup andmagneticneedle+pressinggroup[(42.50~12.41)%,(53.71~10.93)%, (49.10~8.39)%.P<0.O1]. CoNCLUSIoN:Acupuncture.magneticliftingneedle.magneticlifting needlepluspressingplaysaroleinpromotingtherecoveryofrestingpo? tendalofskeletalmuscularcellsafterlong-termelectrostimulation,andthe effectofsimplemagneticneedleissmallerthanthatofacupunctureand magneticneedlepluspressing. XuYM,ZhouXYinXHEffect0facupunctureandmogneticliftingneedleon therecoveryofreslingpotenlialofskeletolmuscleZhongguoLinchuangKan~u 2005;9(421:174—5(Chinal1www.zglckf.coral 矾TR0DUCT10N Themechanismoftheoccurrenceandrecoveryofsportsfatigueis oneoftheimportanttopicsinthefieldofsportsscience}lI.The long.termcontractionofskeletalmusclecanlcadtothedecrease ofrestingpotentialt~l,whichwillcertainlyaffectitscontraction,and resultinfatigue.Thepaperconductedarecoveryexperimentoffa— tiguebyusingacupunctureandinjury—freemagneticneedle,soas toobserveandcomparetheinflueneesofdifierenttreatmentson therecoveryofrestingpotentialofskeletalmuscle. MArERIALSANDMETH0DS Materials Theexperimentwascarriedoutinthelaboratorybuildingofthe DepartmentofBiologicalScience,BeijingUniversityofPhysical Education.Thirtyexperimentalmaleandfemaletoadswiththe bodymassof70-75g,whichwereprovidedbyBeijingUniversity ofPhysiealEducation.wererandomlydividedintoacupuncture group:1o),magneticliftingneedlegroup:10),magneticlift? ingneedle+pressinggroup:1O).Leftandrightsemitendinosus musclesweretakenascontrols. Methods Establishmentofsamples:Thetoads’semitendinosusmusclesof onesidewerepeeled,andthedistaltendonofsemitendinosus muscleswasseparatedintotwoheadsofmuscles.tIlemusclefibers wereisolatedtoproximalend.andoneheadofmusclewasstillre. servedtIlere.Thetwoisolateddistalheadsofsemitendinosusmus— cleswerefixedtoasilastiepad.sothatthesamplesshoweda”V’’ shape. Parametersoftheeleetrostimulation:Thevoltagewas3.5—5.5’V. stringlengthwas0.2ms,frequencywas10Hz,wavewidthwas1 ms,durationwas40minutes. Methodsoftreatments:Theexperimentalmuscleandcontrol muscleinthegroupswerealltreatedwitheleetrostimuladonfor 40minutes.Intheacupuncturegroup,theexperimentalmuscle wastreatedwithobliqueinsertionbyusingtIlefilifoErnneedleof ‘p=0.16mm,andtheneedlewasretainedfor3minutes.buttIle controlmusclereceivednotanytreatment.Inthemagneticlifting needlegroup,theexperimentalmusclewastreatedwithsimplemag- neticliftingneedlefor5minuteswithoutpressure.butthecontrol musclereceivednotanytreatment.Inthemagneticliftingnee— die+pressinggroup,theexperimentalmusclewastreatedwith magneticliftingneedlepluspressurefor5minutes,butthecontrol musclereceivednotanytreatment.ThemuscleeeHsinthe3groups allrestedfor30minutesafterelectrostimulation.andthen山erest- ingpotentialwasrecordedbythemieroeleetrodeamplifier~-51. Statisticalanalysis:ThettestwasconductedbytIlefirstantIlor withtheSPSS11.0software. RESULI’S Quantitativeanalysisoftheexperimentalanimals Allthe30toadsinthe3groupswereinvolvedintheanalysisof results. Statisticalinference Changesoftherestingpotentialofskeletalmusclecellmembrane beforeandaftereleetrostimulation Long—termelectrostimulationcouldresultinthechangesofskeletal musclecellmembrane.andthedecreaseoftherangeofmembrane potential,andmadeitdeveloptodepolarization(Table1). potentialbetweentheexperimental afterelectrostimualtion x:l:s,n=10,mY) .P<n01.”sbeforeeleetrostimualfion;bP>O.o5.”sc蚰帅1mu8ck ISSNl671—5926CN21—1470/R 血M础corn3385083@sinacom徐玉明,等.针刺及磁提针对骨骼肌静息电位恢复的作用 Recoveryoftherestingpotentialofmusclecellstreatedby acupuncturebeforeandafterelectrostimulation(Table2) Table2Effectofacupunctureontherecoveryofrestingpgtential 蜘,10,m? .P(0.01.controlmuscle;bp(0.01.beforeeleetrostimuahion Recoveryoftherestingpotentialofmusclecellstreatedbymagnet— icliftingneedlebeforeandafterelectrostimulation(Table3) Table3Magneticneedleontherecoveryofrestingpotential 蜘,n=10,mV (0.01.controlmuscle Recoveryoftherestingpotentialofmusclecellstreatedbymagnet— icliftingneedlepluspressingbeforeandafterelectrostimulation (Table41 175 medicinebelieves山atmagnetitecanreach山etherapeuticpur— posethroughthewaythatmagnetcanproduceinformationtoad- justmeridian,thebottomofone’sheart,aswellasinnerinfor- mation.Themagneticinduction’sunitintensityofmagneticlift— ingneedleisofhundredstime山atofcommonmagnet.ahdit sendsoutthroughtheneedlepoint,andthepenetrativeabilityis greatlyincreased.Thephysiologicalmechanismofsimplemagnet— icliftingneedleintherecoveryofrestingpotentialisthatthe roleofmagnetcanmakethefunctionofsomeenzymesrecoveror adjustthepenetrationofmembraneandthenleadtotherecovery ofionicconcentration. Afterlong?termelectrostimulation,therestingpotentialofskeletal musclecellmembraneisgreadydecreased.Obliqueinsertionhas anobviouseffectinacceleratingtherecoveryoftherestingpoten- tialofskeletalmusclecellmembraneafterlong--termelectrostimu?- lation.Thesameasacupuncture,simplemagneticliftingneedle andmagneticliftingneedlepluspressingplayanobviousrolein acceleratingtherecoveryoftherestingpotentialofskeletalmuscle cellmembraneafterlong—termelectrostimulation.Thereisnosig- nificantdifferenceineffectbetweenmagneticliftingneedle,simple magneticliftingneedleandsimpleacupuncture. Table4Magneticneedlepluspressingontherecoveryo_ frestingpoten. tial蜘,n=10. mV1REICERENCES .P(0.01.controlmuscle Recoveryoftherestingpotentialofmusclecellstreatedbymagnet- icliftingneedlepluspressingbeforeandafterelectrostimulation (Table5) Table5Recoveredpercentagesofeffectbythe3treatments,n=10) TheqtestshowedthatIP(0.O1.themagneticneedlegroup DIsCUSSIoN Themechanismofacupunctureintherecoveryoftherestingpo— tentialofskeletalmuscleafterlong—termelectrostimulationmaybe thatacupuncturecanregulatetheactivitiesofsomeenzymes,and leadtotherecoveryofthepumpfunctionorthechangesofthe membranepermeability.andthenreachthepurposeofionicrecov. ery.Inthestudy.thereasonforthechangesoftherestingpoten— tialresultedfromtheionicchangesisthesameasforthechanges ofthedelayedstructureofskeletalmuscle. MagnethasbeenusedtotreatdiseasesintraditionalChinese medicinesincelongago.ItissaidinRecordsoftheHistorianthat magnetiteisusedinherbssincetheyearof90B.C.,whichisalso recordedinShenNong’sHerbalClassic.Liftingneedleisoneof the”nineneedles”.itisrecordedinTheYellowEmperor’sInter- nalClassicthataneedleinlengthof山reeandhalfcurtisused withpressingtechniquetomakeqiarrivalandeliminate pathogenicfactors,whichalsocanbefoundinMiraculousPivot. Chapter7andQuestionoftAcupunctureandMoxibustion. Simplemagneticliftingneedleonlyhasmagneticeffect,itisindi- catedthatitseffectontherecoveryoftherestingpotentialof skeletalmusclecellmembraneafterlong—termelectrostimulation ismainlyitsroleofstrongmagneticeffect,however,magnetic liftingneedlepluspressinghasthedoubleeffectsofmagnetic therapyandliftingneedle,itseffectinpromotingtherecoveryis certainlyasgoodassimplemagneticliftingneedle.Magnetic lJosefZ,Chfis~phT,UlrichP,etat.IniluenceofnitrousoxideoffmotorevokedPO. tentials.Spine1997;22:10o2-6 2MarinaRW,NiaurieioRD.NfikhailIs,etat.Somatosensoryevokedpotentialevah. tionofacutenerveiniuryassociatedwithexteroalfixationproceduresClinOrthope. dicsandRelatedRes1994:308:254-63 3McNutonTG,HorchKW.Metafiizedpolymerfibersaslcadwiresandintrafaseie. ularmieroeleetrodes.JNurosciMethod1996:70:103—10 4YoshidaK,JovanovieK,RichardBS.1ntrafascieulareleetredesforstimulationand recordingfrommudpuppyspinalroots.JNurosciMethod2O0o:96:47—55 5BrannerA.NormannRA.AmultieleetrodearrayforintrafascieularrecoIdingand stimulationinsciaticnerveofcats.BrainReBulletin2O0o:51:293—306 6WangRY.YuanYH.FengWQ.ExpressionofMHCisoformsmRNAindueedbyex. hanstiveeccentricexerciseinskeletalmusclesofratsandeffectsofacupunctureon them.如urnatofBeijingUniversityofPhysicalEducation2oo2;25(4):476—9 针刺及磁提针对骨骼肌静息电位恢复的作用? 徐玉明’,周新,殷晓辉(‘山西师范大学体育学院,山西省临汾市 o4l000;山东省滨州学院,山东省滨州市256604) 徐玉明?,男,1969生,山西省临汾市人,汉族,北京体育大学在读博士, 副教授,主要从事运动人体科学研究. 摘要 背景:骨骼肌长时间收缩会导致静息单位的下降,影响其收缩,导致疲 劳发生.针刺以及无损伤的磁提针对静息单位的恢复有无作用? 目的:采用针刺以及无损伤的磁提针作为恢复手段.观察和比较其对骨 骼肌静息电位恢复的影响. :对照实验. 单位:山西师范大学体育学院,山东省滨州学院. 材料:实验蟾蜍3O只(由北京体育大学实验动物中心提供),随机分成 针刺组,磁提针组,磁提针加按压组,每组1O只.左右半腱肌进行对照. :对离体蟾蜍半腱肌(实验肌和对照肌)进行40min电刺激后,采 用细胞内玻璃微电极技术测量骨骼肌细胞的静息电位.并且在电刺激 后进行毫针针刺,磁提针和磁提针加按压等处理. 主要观察指标:各组运动前,电刺激后以及处理后的静息电位. 结果:?电刺激后骨骼肌细胞膜静息电位较电刺激前下降『实验肌: (59.48~7.37),(84.54~572)mV,对照肌:(59.43~7.14),(83.62~5.86)mV]. ?针刺,磁提针,磁提针加按压使骨骼肌细胞电刺激后下降的静息电位 明显恢复(P<0.O1).?磁提针组静息电位恢复百分比明显小于针刺组 和磁提针加按压组【(42.50~12.41)%,(53.71~10.93)%,(49.10~8.39)%, P<0.01l. 结论:斜刺,磁提针,磁提针加按压对长时间电刺激后骨骼肌细胞膜静 息电位的恢复有促进作用.单纯磁提针对长时间电刺激后骨骼肌细胞 膜静息电位恢复的促进作用小于针刺和磁提针加按压作用. 主词:针刺;磁提针;磁提针加按压;静息电位;细胞内微电极技术 中图分类号:R337.5文献标识码:A文章编号:1671-5926(2005)42-0174-02 徐玉明,周新,殷晓辉斜刺及磁提针对骨骼肌静息电位恢复的作用”I.中国临床康 复,2005,9(42):174-5[www.zglckf.coml fEditedbyWangDH/YinYjH/WangL)