三碘甲状腺原氨酸对人神经干细胞分化过程中甲状腺激素受体表达的干预效应_英文__cropped

三碘甲状腺原氨酸对人神经干细胞分化过程中甲状腺激素受体达的干预效应_英文__cropped h u m a n e m b ryo n ic b ra in -d e rive 122 Liu C h u n -ron g , Li Lan -yin g , Liu B en , Z an g X ia 1 D epartm ent of Abstract P athology,Tianjin M edical C ollege of BACKGROUND: Triiodothyronine (T) is an im portant3 C hineseP eople's controlthe successive differentiation during the deve A rm ed P olice Force, Tianjin 300162,2 C hina; Institute of OBJECTIVE: To m onitor the differentiation of neura E ndocrinology, Tianjin (TR ) m R N A expression changes. M edical U niversity, Tianjin300070, C hina DESIGN: O pen experim ent. Liu C hun-rong ? , M aster, Lecturer, SETTING: D epartm ent of P athology, Tianjin M e D epartm ent of T Rm R N Ae x p re ssio na lte ra tio nd u rin gth e d iffe re n tia tio n o f N S C s TR αm R N A expression levelw as the highest before inducing N S C s.1 W ith the induction the expression level w as decreasedof T,3 gradually, and w as the low estat2 w eeks, and then w as rebounded gradually, but the finallevelw as stilllow er than that of N S C (F = 32.49,P =0.008). The tendency ofTR αm R N A expression alteration2 w as identicalw ith thatofTR αm R N A . TR βm R N A expression level1 1 w as the low estin N S C , w as increased gradually w ith the induction of Tand attained the highest level at 2 w eeks of induction of T.3 3 Furtherm ore, the expression level of TR βm R N A w as also higher1 ,P = 0.001), and it(t =15.64 than that of TR αat the sam e tim e1 reached the low estlevelat3 w eeks after induction. TR αexpression 3 levelw as firstly decreased after the differentiation induced by T, and3 w as close to the expression level of N S C at 2 w eeks of induction(F =51.94,P =0.378) and decreased to a low er levelafter 3 w eeks.T3 induced TR m R N A expression during the differentiation of N S C , and agarose electrophoresis im age is show n in Figure 5.