猪肌肉糖原酵解潜能及其组分与肉质, 脂肪沉积和血液性状的相关性

猪肌肉糖原酵解潜能及其组分与肉质, 脂肪沉积和血液性状的相关性 猪肌肉糖原酵解潜能及其组分与肉质, 脂肪沉积和血 液性状的相关性 2010, 32 6 : 1224 - 1229 / / xuebao. jxau. edu. cn A cta A gr icu turae Un iversitatis J iangx iensis E - m ai: ndxb7775@ sina. com 段艳宇, 周利华, 袁 飞, 李 琳, 麻骏武1* /, 330045 : F2 , GP * : G P pH24 h r - 0. 39, P 0. 001 b r 0. 36, P 0. 00 1 L * r 0. 13, P 0. 001 , GP , pH , pH4 5m in r - 0. 59, P 0. 001 T 45 m in r 0. 24, P 0. 001 L* r 0. 34, P 0. 00 1 r 0. 32, P 0. 001 , , pH , GP , GP , , : ; ; ; ; : S828. 211 : A : 1000 - 2286 2010 06- 1224 - 06 Correlation ofMuscle Glycolytic Potential and Its Componentswith M eat Quality, FatDeposition and Blood Indexes in Pigs * DUAN Y anyu, ZHOU L ihua, YUAN Fe,i L I L in, MA Junw u Candidate of N ationa K ey Laboratory for A nmi a B iotechno ogy of Ch ina, J iangx i A gricu tura Un iversi ty, N anchang 330045, Ch ina A stract: T he ami of the study is to ana yze the co rre at ion o f m u sc e g yco yt ic po tentia GP and its com ponen ts w ith m eat qua ity, fat deposit ion traits and b ood indexes, u sing the pheno typ ic data obta ined from a arge - sca e W h ite Duroc E rhua ian F popu ation. Sign ificant corre at ions w ere found am ong som e o f 2 these traits. GP of the Longissmi u s dorsiw as co rre ated w ith pH 24 h va ue r - 0. 39, P 0. 001 , M ino ta * * b va ue r 0. 36, P 0. 001 and L va ue r 0. 13, P 0. 001 , ind icat ing GP a ffected u tmi ate pH and m eat co our. S mi i ar y, the corre ation o f actic ac id content in mu sc e w ith pH45 m in va ue r - 0. 59, P * 0. 001 , T45 m in va ue r 0. 24, P 0. 001 , L va ue r 0. 34, P 0. 001 and p igm ent con tent r 0. 32, P 0. 001 indicated that actic ac id conten t w as associated w ith faster dec ine o f pH, h igher in it ia temperature of m eat and pa erm eat co our. In add it ion, GP and actic acid conten tsw ere positive y and signif icant y corre ated w ith fat am ount of subcutaneous and v iscera adipose t issues. H ow ever, non- significan t or s ight corre ations w ere observed betw een GP and b ood indexes. T aken together, the resu ts of this study sug gested that GP and its componentsm ay affect m eat qua ity and had a itt e effect on fat deposition traits. : 2010 - 06- 10 : 2010 - 10 - 26 : 0530055 G JJ09470 : 198 1- , , , , , E - m ai: yanyuduan@ hotm ai . com; * : , 6 : ! 1225! Key words: p ig; corre at ion; g yco yt ic potent ia; m eat qua ity; fat deposit ion tra it G yco ytic po tent ia, GP [ 1] GP pH , , pH , , GP , , [ 2] , GP , , GP PRKA G 3 3; RN- R200Q [ 3] ; , GP , 100 [ 4- 5] , , F 700 2 1 1. 1 2 17 , F 1 9 59, 1 912 F : 967 ; : 94 5 M i an 2 [ 3] - F PRKA G 3 R200Q RN , RR 0 F 46 90 , 120 2 m 2 / 2 , , 120 , 210 g /kg 3 300 kca 125 g / kg, 120 160 g / kg 3 100 kca 7. 8 g / kg , 1. 2 1. 2. 1 GP糖原乳酸 30 m in , 14 / 15 20 g, , , - 80 Passonneau L ow ry[ 6] No [ 7 ] GP 2 + + + , GP 1. 2. 2 pH 值和温度 , 12 / 13 , 45 m in3 h9 h15 h 24 h pH ; 4 5 m in3 h9 h 15 h M ett er T o edo De ta 320 pH , , pH pH pH 7. 00 4. 0 1 pH , 4 1. 2. 3 其余肉质指标 24 h, 15, 4 , 24 h NPPC 6 [ 8 ] * * , 1 , 6 ; M ino ta CM - 2600d / 2500d 3: L a * [ 9] b NPPC 10 , 1 , 10 1. 2. 4 脂肪沉积性状 [ 10] 1. 2. 5 血液性状 , , - 80 , 7600 , : , , HDL - LDL - ! 1226! 32 , 1. 3 SA S 9. 0 SA S Inst itute IN c. , Cary, NC, U SA. 2002 F 2 1 CORR , Pearson 1 F 2 Ta . 1 Simple statistical analysis ofm eat quality, fat deposition and lood indexes in F population 2 T ra its N o. M eans S tv M in M ax GP and its com ponents / / m o ! g- 1 G ycogen / g ucose 706 26. 22 13. 8 - 0. 99 81. 15 / m o ! g- 1 G ucose- 6- phospha te 706 0. 19 0. 39 - 1. 17 4. 8 / m o ! g- 1 L actate 706 82. 48 20. 12 27. 35 160. 13 / mo ! g- 1 G yco ytic potentia 706 135. 4 29. 31 27. 84 262. 94 M ea t qua ity of LM T45 m in 750 34. 2 1. 83 26. 3 39. 5 T 744 19. 96 4. 07 8. 6 32. 2 3 h T9 h 659 8. 86 4. 08 0. 4 24. 7 T 635 5. 56 3. 18 0. 1 18 15 h pH 45 m in 739 6. 4 0. 34 5. 34 7. 11 pH 3 h 735 6. 26 0. 44 5. 27 7. 13 pH 9 h 742 5. 92 0. 33 5. 32 6. 78 pH 15 h 74 5 5. 75 0. 23 5. 31 6. 66 pH24 h 753 5. 67 0. 17 5. 35 6. 64 L* 760 47. 21 3. 13 36. 75 59. 14 * 76 1 0. 73 1. 15 - 2. 57 5. 67 a * 76 1 7. 56 1. 83 2. 15 13. 22 b - 1 / g! g H aem atin content 759 46. 84 17. 51 4. 76 132. 27 Co our scores 1~ 6 762 2. 68 0. 72 1 5 M arb ing sco res 1~ 10 762 1. 92 0. 73 1 10 F at deposite tra its / g P erirena fat w e ight 1 028 2 08 1 1 096 70 6 055 / g M esen teric fa tw e ight 1 028 1 30 1 442. 39 217. 50 3 348 / g A bdom ina fa tw e ight 1 028 1 227 425. 16 112. 50 2 7 38 / cm B ackfat thickness a t 1st rib 1 031 3. 92 0. 95 1. 34 7. 30 / cm Backfat th ickness at 6~ 7 th rib 1 031 3. 13 0. 96 0. 14 6. 85 / cm Backfat th ickness at 1st um ba r 1 031 2. 34 0. 86 0. 21 6. 93 / cm Backfat th ickness at h ip jo int 1 031 2. 55 1. 02 0. 24 7. 01 B ood index / mm o ! L- 1 B ood g ucose 759 5. 31 2. 13 0. 20 14. 30 / mm o ! L - 1 G ycosy ated serum prote in 759 1. 18 0. 23 0. 50 3. 10 / mm o ! L- 1 To ta cho estero 759 2. 57 0. 62 0. 18 4. 13 / mmo ! L- 1 T rig yceride 759 0. 28 0. 15 0. 07 1. 18 / mm o ! L- 1 H igh - density ipopro te in 759 1. 13 0. 23 0. 50 2. 00 / mm o ! L - 1 Low - dens ity ipoprote in 759 1. 30 0. 27 0. 60 2. 47 T: ; LM: ; T: temperature; LM: Long issium m u sc e. 6 : ! 1227! 2 2. 1 GP 2 , T45 m in, T45 m in , , GP pH45 m in, pH3 h, pH9 h, pH15 hpH24 h, GP pH 24h - 0. 39 P 0. 001 5 pH , pH 45 m ni - 0. 59 P 0. 00 1 pH pH ; pH45 m in 0. 38 P 0. 001 , pH24 h- 0. 20 P 0. 001 pH * * * * GP L b , GP L b * * * L b GP L * ; , GP 0. 32 P 0. 001 0. 06 P 0. 05 , L GP * 0. 34 P 0. 001 0. 13 P 0. 001 b , * * * M ino ta L r - 0. 20 a r - 0. 16 b r 0. 23 r 0. 11 , 2 Ta . 2 Correlation coefficients etw een glycolytic potential and otherm eat quality traits / T ra its G ycog en / g ucose G ucose- 6- phosphate L actate G yco y tic po ten tia T *** *** *** 4 5m in - 0. 27 - 0. 16 0. 24 - 0. 10* T - 0. 05ns - 0. 06ns * 0. 01ns 3h 0. 10 T * - 0. 02ns *** 0. 00ns 9h - 0. 10 0. 14 T - 0. 0 8ns 0. 01ns 0. 07ns - 0. 03ns 15h pH *** *** *** - 0. 03ns 4 5m ni 0. 38 0. 24 - 0. 59 pH *** *** *** - 0. 07ns 3h 0. 32 0. 27 - 0. 56 pH *** *** *** * 9h 0. 22 0. 25 - 0. 4 8 - 0. 11 pH 0. 04ns *** *** *** 15h 0. 18 - 0. 4 1 - 0. 24 pH *** - 0. 06ns *** *** 24h - 0. 20 - 0. 30 - 0. 39 * ** *** *** *** L - 0. 10 - 0. 20 0. 34 0. 13 * 0. 07ns *** 0. 06ns ** a - 0. 16 0. 10 * *** *** *** *** b 0. 16 0. 23 0. 29 0. 36 / g! g- 1 *** *** - 0. 17 - 0. 04ns 0. 32 0. 06ns H aem atin content *** ** * Co ou r sco res 1~ 6 0. 14 0. 11 - 0. 09 0. 07ns - 0. 0 3ns - 0. 06ns - 0. 0 3ns - 0. 05ns M arb ing sco res 1~ 10 ns; * P 0. 05; ** P 0. 01; *** P 0. 00 1 ns, non- sign if icant; * P 0. 05; ** P 0. 0 1; *** P 0. 001. 2. 2 GP 3, GP , GP ; / ! 1228! 32 3 Ta . 3 Correlation coefficients etween glycolytic potential and fat deposition traits / G ycogen / g ucose G ucose- 6- phospha te L acta te G y co ytic potentia / g Per irena fat w e ight - 0. 06ns - 0. 01ns *** *** 0. 25 0. 13 *** * / g M esenter ic fat w eigh t - 0. 0 8ns - 0. 03ns 0. 20 0. 07 / g A bdom ina fat w eight - 0. 0 3ns - 0. 01ns *** *** 0. 20 0. 12 / cm *** ** - 0. 05ns - 0. 01ns 0. 19 0. 09 Back fat th ickness at 1st r ib / cm - 0. 05ns - 0. 02ns *** * 0. 15 0. 07 Back fa t th ickness at 6~ 7th r ib / cm *** *** - 0. 0 3ns 0. 01ns 0. 18 0. 11 Backfa t th ickness at 1st umbar / cm 0. 05ns 0. 04ns ** *** 0. 10 0. 12 Backfa t thickness a t hip jo int ns; * P 0. 05; ** P 0. 01; *** P 0. 00 1 ns, non- sign if icant; * P 0. 05; ** P 0. 0 1; *** P 0. 001. 2. 3 GP 4 , GP GP , 0. 1, 4 Ta . 4 Correlation coefficients etw een glycolytic potential and lood indexes / G ycogen / g ucose G ucose- 6- phospha te Lactate G yco ytic potentia *** B ood g ucose 0. 07ns - 0. 02ns - 0. 19 - 0. 07ns G ycosy ated serum prote in 0. 06ns 0. 00ns 0. 05ns * 0. 09 T o ta cho este ro 0. 03ns * *** * 0. 09 - 0. 16 - 0. 09 T rig yceride 0. 01ns - 0. 01ns 0. 00ns 0. 01ns H igh - density ipopro te in 0. 02ns - 0. 05ns 0. 0 3ns 0. 04ns Low - density ipopro tein * * - 0. 06ns ** - 0. 08 0. 09 - 0. 12 ns; * P 0. 05; ** P 0. 01; *** P 0. 00 1 ns, non- sign if icant; * P 0. 05; ** P 0. 0 1; *** P 0. 001. 3 3. 1 GP [ 4] * GP pH H am i ton GP L * * * b , GP L b , [ 11] * * 2003, M oe er GP L r 0. 40 b r 0. 43 2002 , H am i [ 12] * ton GP pH , , GP L * [ 13] L , L onergan [ 14] Rosenvo d , 24 h , [ 15] pH GP pH , T45 m in, [ 16 ] , , B riskey 6 : ! 1229! 3. 2 GP , , , , [ 4 ] 2003 H am i ton GP [ 15] r - 0. 27, P 0. 05 , , , GP , 3. 3 GP , , [ 17] , , , , GP/ , , GP : [ 1] M on in G, Se ier P. Pork of ow techno og ica qua ity w ith a no rm a rate o fmu sc e pH fa in the mi m ed ia te po st- m ortem pe riod: the case of the H am psh ire breed[ J] . M ea t Sc i, 1985, 13: 49- 63. [ 2] . [ J] . , 2002 2 : 33- 34. [ 3]M i an D, J eon J T, L ooft C, et a. A mu tation in PRKAG3 assoc iated w ith ex cess g ycog en conten t in p ig ske eta mu sc e [ J] . Sc ience, 2000, 288 5469 : 1248- 1251. [ 4] H am i ton D N, M i er K D, E isM, et a. R e ationsh ip s be wt een ong iss mi u s g yco y tic po tentia and sw ine grow th perform ance, carcass tra its, and pork qua ity[ J] . J A n mi Sc,i 200 3, 80 3 : 617 - 627. [ 5]W a rr iss P D, Bev is E A, Ek ins P J. The re ationsh ip s betw een g ycog en sto res and m u sc e u tmi ate pH in comm ercia y s aughte red p igs[ J] . Br ih V et, 19 89, 14 5 4 : 378- 383. [ 6] Passonneau J V, Low ry O H. Enzymatic ana ysis- a practica gu ide[M ]. The H uman Press Inc, New Jersey, U SA, 1993: 9- 10. [ 7] N o F. M ethoden de r enzym a tischen A na y se 2nd [M ] . V er ag Chem ie GmbH, W e inhe mi , FRG, 1970: 1433. [ 8] N PPC. O ff ic ia Co o r andM a rb ing S tandards[M ] . N at P ork P rod Counc i, D esM o ines, IA, 1999: 76- 83. [ 9] H ornsey H C. The co o r o f cured- cooked po rk. I. Estmi ation o f the nitric- ox ide- haem p igm ents[ J] . J Sc i Food A gr ic, 1956, 7 8 : 734. [ 10]. [ M ] . : , 1995: 165- 169. [ 11]M oe e r S J, B aas T J, L eeds T D, e t a. R endem en tN apo e g ene effects and a com parison o f g yco ytic potentia and DNA g enotyping fo r c assif ication o f R endement N apo e statu s in H amp sh ire- sired pigs[ J] . J A n mi Sc,i 2003, 8 1 2 : 402- 4 10. [ 12]H am i ton D N, E is M, H emann M D, e t a . The mi pact o f Long issm u s g yco ytic po ten tia and short- term feed ing o f m agnesium su fate heptahydrate pr ior to s aughte r on carcass characte ristics and po rk qua ity[ J] . J A n mi Sci, 2002, 80 6 : 15 86- 1592. [ 13]H uff- Lonergan E, Baas T J, M a ek M, e t a . Corre ations am ong se ected pork qua ity tra its[ J] . J A nmi Sc,i 2002, 80 3 : 617 - 627. [ 14] R osenvo d K, Pe tersen H N, Lerke S K, e t a . mu sc e g y cogen sto res and m eat qua ity as a ffected by stra teg ic f inish ing feeding of s aughte r p igs[ J] . J An mi Sci, 2001, 79 2 : 382- 39 1. [ 15] , , . [ J] . , 2008, 44 1 : 25- 29. [ 16] B riskey E J, K astenschm idt L, Forrest J C, et a. B io chem ica A spects of P ost- M ortem Changes in P orcine M u sc e[ J] . J A g ric Food Chem, 1966, 14 3 : 20 1- 203. [ 17], , . [M ] . 3. : , 2005: 157.