第八章 动物细胞培养和生长过程

第八章 动物细胞培养和生长过程 第八章 动物细胞生产和培养过程 8.1背景 尽管先前有许多调查者研究了动物细胞在试管中的习性。但是。1949年，这种能产生有用物质的动物细胞首次被利用。在当时，J.F Enders 证明了脊髓灰质炎病毒在培养的动物细胞中可以生长，而这种细胞源于灵长类动物的神经和非神经组织中。 简洁的总接一下这一伟大的探索工作，开始与19世纪80年代早期。根据论证白细胞能在躯体外分裂。这种细胞是通过观察注入在血清、淋巴或腹水中以切成片状的动物组织所得。(1907年),(H,,,,,,,的悬在下面的淋巴当中的(蝌蚪骨髓)组织。而这种盖玻片密封在一个中间挖空的载玻片上。这项伟绩(,,,,年)由,,,,,,所继承和发展。他研发了一种维持外来物质特别是细菌培养的复杂方法，其他人很少有这种能力，然而，截至,,,,年，培养基的发展促进动物细胞的增长以至于,,,,,,,,,能够在装有切碎的老鼠或小鸡胚胎的试管中培养病毒。,,,,,,在脊髓灰质炎的工作中运用此方法。脊髓灰质炎的作用是有他和他的同事通过使用抗生素获得相当多的帮助，这些抗生素在十年前是非常流行的，,,世纪,,年代早期Salk发明的脊髓灰质炎疫苗是由猴子的肾和睾for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet 丸细胞在试管培养中生产的。一旦这种技术被确立，培养中生长的小鸡或其他原始的胚胎细胞生产其他疫苗。【麻疹病毒(,,,,年)。流行性腮腺炎病毒(,,,,年)、腺病毒(,,,,年)】 ,(,从动物细胞培养中获得的不同产物 动物细胞过去是现在仍然是从培养动物细胞中获得最具有商业性的产物，目前，每年生产大约,(,,,,,剂量的口啼疾疫苗，近似于纽卡斯尔病和马立克氏病的家禽疫苗。人类病毒疫苗以每年,,,剂或者较低一点的速度在运用。干扰素生产的过程也是一大规模，接近或超过这些运用于兽医疫苗的情况下，然而有利于具体的合成杂交瘤细胞相对于免疫学方面是毫无疑问的，在未来的十年里是举足轻重的。表,。,中列出已经或者可能在培养动物细胞过程中获得的主要产物，这并不是唯一的产物，而是表现着主要产物以及能在这些区域内获得的物质的类型。 ,(,产物生产方法的综述 图,。,到,。,概括的描述了对于动物细胞培养体系中产物的发展。它包括三个阶段，第一个阶段是准备阶段，第二阶段是动物细胞培养阶段，第三阶段是产物的产生阶段。早某种体系上(荷尔蒙和免疫生物产物)最后一阶段是区别于单元细胞的生产方式。然而在其它方面，最后一阶段跟病毒细胞培养的传染有关或与诱生剂有关。(如干扰素，for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet 见表,。,) 对生产经营的很多努力涉及到质量控制系统，与此同时，在线和离线在生物技术领域中随处可见。质量控制测试的不仅能够确保使用的物质能够促进细胞或产物的发展，还能确保他们摆脱外来的生体，例如，污染物 图表,。,清楚的表明生长培养基的准备工作是一项艰难而又需要专门的技术的过程，当培养基所确定的组份容易控制时，最有困难的两种组份是水和血清。 水可以从多种资源中获得，甚至蒸馏和去离子作用后，水也可能包含着对细胞生长不利的物质。对此，格陵兰冰岛底部所融化的病是一种很有用的参考物质，贮备水的缺乏带来一些困难。细菌能在室温蒸馏水中达到,,,单元的含量，同时远离组成的变量，这种细菌的产物少部分可能是有毒的，在,,C贮藏的水通常被攻克，在这个温度下表面将无菌。 也可以制备没有血清的培养基，但并非所有的细胞可以再生。然而这种细胞培养基的结合对密封材料的产生没有多大用处。 以去除免疫球蛋白成分的血清在病毒生产体系方面最有用这种病毒生产体系是通过当地生产的血清包含的对生产病毒的抗体的一种体系，虽然买的和做实验的血清的价格不菲，但是室内生产是有可能的，血清质量的控制的彻底性for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet 对可靠的重复运转是很有必要的。在零下,,C贮存预先测试的血清是经常可取的，因为他能够代替新鲜血清质量减退的这些时光，(通常夏天较容易)。牛胎血清经常被应用于细胞生长发面，但是牛胎血清既贵又罕见。带有噬菌体支原体这样的物质也是最可靠的。也能成批成批的管擦牛胎血清的生长特性，由于来自幼地鼠地肾的细胞，,,,利润从成年牛的血清也可以得到。\ 动物细胞能生长在两种不同类型的培养基上，如果多数动物细胞首次被运用到表层或者固体基质。那么他们可以被诱导分裂，正好相反，一些可能在悬浮液中被诱导自由生长，前者被称为单层细胞，应为他们在基质上形成一层厚壁细胞，即使在连续培养的添加组织也能形成，依赖基质生长的细胞被称为是悬浮细胞或非贴壁依赖性细胞，。 我们认为只是生长的单层细胞比悬浮细胞有特别少的致癌基因(能使细胞致癌的基因序列)，因此对于人类的消耗，这种细胞被用于生产物质，多种也证明此种 细胞比在悬浮液中的生长的细胞有能力生成更多的病毒毒性。但是如病毒或是产物从悬浮生长的细胞生产制造，那么多数过程会相对容易些，兽医所使用的产物按这种方法所得(脚嘴病毒)，同时也包括a-干扰素以及有关杂交瘤细胞的免疫学生物特性。尽管在选择测试体系方面，后两种产物所使用的细胞被证明是可以致癌的，但是在生产阶段不会有什么危害，for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet 或者是优先使用单位细胞的产物。在表8.2中，有一个对于单层细胞使用有利和不利条件的总结。我们可以得出结论，在细胞类型方面能够生产商业性细胞是很有必要的。并且也需要两种必要的但不同的技术，下面将陈述两种不同的技术。 8.4单层细胞培养系统 获取基质的生产细胞系统可以被划分为两种类型，一种是增加设备单元的数量以扩大进程;另一种是多重和过程。通过增加设备的尺寸来实现扩大 8.4.1单层细胞生长的多重过程 传统的来讲，固定的玻璃瓶或者是培养皿内已经生成了单层细胞，这种瓶子有许多结构，被命名为brockway，roux ,Carrel,Baxter或Medical Flats，钠玻璃可能被使用，但是硼硅酸盐会更受欢迎，因为他尽管越复杂，但越坚硬越容易修理。那玻璃瓶也许会单独使用，但是经常固定在搁架上便于运输。能够使细胞生长在瓶的内表面而不是瓶底的系统，它的发展同样是滚瓶系统的发展。尽管所使用的矩形瓶和十字型瓶也能够转动，但是对于这个系统，圆柱型瓶被广泛使用，有许多瓶子能够达到180cm长，滚筒上边放着瓶子，而这样的滚筒放在机械支架上。一套标准的设备可以转动12个瓶子，6~10套支架可以组成一个生产单元，在意大利布雷西亚的Zooprofilattico Sperimentale大学学院有7000个瓶子for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet 在每四个培养箱里可同时转动。再次，滚动瓶系统被发展到了极限，对于已大量瓶子为媒介的细胞和病毒的生长同时还要保持无菌培养，所以这种增长是很难克服的，布雷西亚单元高度的自动化装置提高了这种方法的经济可行性。 8.4.2单层细胞生长的单元程序 20世纪60年代中期，关于单层细胞的培养单元程序得到发展，同时单元程序的发展现在已变成一项易于接受的首选的实用技术。最近，其他方面的创始人重新检查了这一系统的许多细节，结果跟随最近的发展，不同的系统将在下边描述 根据可靠的成功的多重过程的运转，被设计取代他们的单元模式跟随多重系统的基本模式，这样，在器皿中的一套弹簧片单元程序得到发展，同时使人联想到一架子的瓶子，这样的系统有一次性的聚苯乙烯、聚碳酸酯和钠玻璃组成。后期的发展是采用一叠盘子同时以直接构造的形式转动他们，要么以水平的形式转动他们，后期系统正在扩大密集利用的情况下，目前作为B或纤维素细胞干扰素的一种方式，在清理与细胞培养运行期间，这种系统的扩大反映着一系列技术困难与操作问题，按照这种方式装入容器的表层数量很有限，却有两种结果: (a)磁盘双方都被使用， (b)以稀释形式生产产物正如生长培养基冲洗过后，for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet 而培养基要么达到饱和程度，要么至少半饱和程度，总共达到外面容器的容积。从已经发展的大范围多试管细胞的繁殖，我们看到了滚瓶系统的发展，系统达到2001量的大规模发展，近期的一套细胞大量生长的设备，Gyrogen有一排平行的试管组成，这种试管是从1001的量结合外界圆柱形壳在外部力量的驱动下转动。这套昂贵的设备可用来生产，但是是否合理的利用能得到经济上有力的价值好没有被证实。半空纤维中的试管收缩是唯一导致生物量的发展。对小规模来讲，在外界纤维以细胞生长的方式刺激动物组织结构或者通过纤维腔来促使营养培养基的渗漏。这是非常有可能实现的，在两种意义上，这种系统有些优势，正如废弃物质(含有细胞毒素)，和产物能够从细胞当中分开，这种细胞是通过纤维的半透膜产生的。最近，这种自然系统已经发展到了0.21，同时在良好的发展计算机控制和检测系统得到了商业化的应用和发展。 目前，为了细胞和产物的生产，许多项目组利用填充层，例如B干扰素，口蹄疫病毒和猪水疱病病毒。经过玻璃球瓶内的聚苯乙烯小白球随着弹簧不锈钢带的变化而变化，然而，伴随着大多数便利的集中在辅助培养基贮藏中控制活性(PH和二氧化碳)，通过循环流动的培养基，最基本的填充曾在多数系统中是很普通的，他们相对容易和扩展，这是由于处理圆柱形填充的机械故障很容易得到排除处理，正如玻for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet 璃作为一种基质。操作的可靠性得到提高，同时实验室研究的 在玻璃表层和细胞之间的关系也没有改变，更愿意不来说，当在一个熟悉的基质中形成产品时，控制新的生物产品的生产者几乎没有多大问题。 很显然，静态填充床没有他们自己的问题。跨层的斜度是相同的，检测培养细胞有一些难度，但不能放在显微镜下观察。通过检测循环的培养基的成分，(细胞生长的葡萄糖浓度，细胞分裂的乳酸脱氢酶活性好最终病毒解体)，尽管后边的问题容易处理，但是不均一的多项系统问题仍然存在。 A(J.van Wezel发展了对于单层动物细胞生长的同种系统。1967年它表明细胞以火胶棉镀膜能生长在0.2mm的葡聚糖纤维素A50颗粒上，凭借微小的搅动，这种颗粒可以成悬浮状态，相对密度为1.05，同时当系统从它本身的方式搅拌时，细胞可以吸附在这种为载体上，由于这些最初的观察，众多的观察者已经表明可以达到5001的量。生长在为载体上的细胞产生物质是有可能的。最新表明，来自非洲的猿猴肾细胞脊髓灰质炎病毒的产量是存在一个现代的为载体表面。其他为载体有聚苯乙烯、聚丙烯甚至是玻璃微球体。然而，在大多数情况下似乎葡聚糖为载体提供了最合适的基质。 众多研究者已经能够成功可靠的用为载体系统来操作，for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet 然而，有些研究人员的实验还有很大的困难，涉及到研究者所希望生成的特殊细胞任然有些困难。特殊细胞本身是集体组成的产物，以及他生成次生产物的方法问题。源于其他合适设备与不合适设备的不当使用以及培养基选择的不恰当问题上。这些选择阶段或者是细胞与玻璃粉。首次关键性的联合或者时候起贴壁细胞的可重复性。 总之，对于基质有要求的细胞的生产，在此环境下，微载体成为首选方法。而且操作良好并且可靠，但是玻璃球的填充层能够提供一个可靠的后补系统。其他系统也会有一定的位置，为准备一种独特的细胞类型能够生长和生产产物提供的特殊的环境。 8.4.3悬浮细胞的生产过程 悬浮动物细胞的培养过程不同于细菌的培养，一条近期的评论描述了许多需要检测和控制的参数，所使用的设备是一个有较低的搅拌和通风度相当标准的发酵器。把传统方法当作一个系统，并且此系统已经增大到8000L。来自于幼地鼠肾细胞货真不同细胞的用于口啼疫病毒的商业产物，如此规模得益于仓鼠，也就是所谓的2FFA-3.同样，有Nsmalwa淋巴细胞所产生的a-干扰素，目前以同样的规模的相似设备来生产。搅拌驱使系统的最基本的修改等同于气升式发酵罐的运用。通过在发酵罐底部引进气泡来提供三料的混合。因为这些在移动也提上是非常有效的而且不会破坏细胞。传统for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet 的喷射会对动物细胞引起严重的破坏，这种喷射通过产生一些小的气泡试图增加气泡的表层区域的面积。溶氧水平在标准状况下本身下降15%一下或者上升到溶氧量以上才会有害。但在气液接口处的相互作用的细胞可能会导致细胞活性的损失。令人惊奇的是，起因于叶轮的飞快的速度造成的物理搅拌似乎没有多大伤害，然而，对于相关参数的效应和数量的研究中需要被保留着。 当传统的的不锈钢槽作为科技的主导时。科技不发达的国家可能会修改一些系统。对此通过外磁条力量来驱动，101 6搅拌瓶可以制造出许多有用的疫苗，(每年均有5*10剂)。印度尼西亚的泗水市安装了此系统。在此地，每个月将产生250000剂疫苗。同样Polgolla和斯里兰卡基本设备的安装也很到位。 生产细胞还是为这些细胞收集信息，动物细胞的连续培养是可行并且有用的方式。最近研究已经表明了在系统的计算机在线监测和控制的优势。购买相应的计算机硬件设备也比较容易。 然而动物细胞的连续型生产利用以及具有商业吸引力的细胞产物还，没有实现。 8.5后期过程 当动物细胞应用于原材料生产时，许多后期过程等同于蛋白质化学技术的其他领域。仍然考虑到存在些更多的独特for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet 细节的操作系统。例如，过滤、筛选操作，离心分离操作(适速)，沉降操作，包析发的层析操作以及浓缩干燥(反渗透法)，这些普通的加工技术很难应对，生物病毒物质的钝化，整个病毒颗粒活性子单元的产生以及用于有用物质的形成具有划时代意义。而这种划时代是以动物细胞为根据的独特的处理工程。 8.5.1钝化作用 尽管许多疫苗是由易感染的病毒粒子形成，但是一些被杀死或钝化。钝化在确保接受着没有没有危险是非常重要的 ，钝化药物通常使用甲醛，然而用B—丙醇酸内酯来钝化狂犬病毒。进来。用亚胺(乙炔和吖丙叮)来钝化脚口疾病病毒。然而亚胺会使脚口疾病病毒的结构发生轻微的变化。甲醛与衣壳体交联形成更稳定的结构。其他钝化法的紫外线辐射和缩水甘油醛，在所有钝化方法中，确保钝化的病毒具有单分散性是非常重要的，以至于失活剂不能阻止病毒在中型团块中有效的钝化。 8.5.2亚基的形成 病毒疫苗必须考虑接受疫苗者的危险，如果不适当的钝化可能会形成亚基。依靠亚基的裂解最终将会形成一小部分致疾病的病毒。能够形成亚基的病毒有狂犬病毒，肝炎病毒、单纯性疱疹、巨细胞病毒和流感病毒。近来，尝试从流感病毒中分离血凝素糖蛋白免疫原表明疫苗的有效性了、，尽管有for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet 效性和天然病毒不同，到目前为止，研究表明从病毒中分离出的糖蛋白的竞争性比原始病毒低。但当足够的材料管理可以得到足够的保护反应。 8.5.3产品配方 3活的病毒疫苗通常包含1~4种不同类型的病毒。每剂有10活性病毒，有些材料需要冷冻干燥保存再生物保护剂中像流感病毒，人或牛的牛痘病毒或血清蛋白谷氨酸盐和磷酸盐离 11子。另一方面，杀死的病毒疫苗大约10为一剂.这些混合物被称为佐剂，佐剂有强效的生物免疫原特性。佐剂的化学组成是不均等的，像强心苷、皂素、各种铝盐(氢氧化物，硫酸盐。磷酸盐)和一些确定的油脂，与水中悬浮的病毒均匀的发生生物交联结合形成水油乳状混合物。油水乳状物在含有去垢剂的无机盐水溶液中经过第二次乳化作用。这些物质的作用许多是一致的，他们促使身体和化学免疫系统最大速率合成大量的有用抗原。 8.6 基因工程动物细胞和细菌 在原核细胞和真核细胞中插入确定的核酸序列的能力 对于细菌和动物细胞具有很广泛的意义。作为一种产物， 蛋白质能够插入特定的核酸。而基于动物细胞的活的病 毒疫苗形成过程不可能取代基于基因工程细菌的过程。 这是清楚的，一些动物细胞生产的胰岛素和一些α-干扰 素商业上用原核生物生产。另外一些像用于口蹄疫病毒for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet 的死的病毒疫苗，骨髓灰质炎和各种干扰素商业上可以在细胞类型生产。然而，基因工程动物细胞能够使蛋白质糖基化成为更受欢迎的基质用于人生长激素，马血溶性酶诱导物，组织血纤维蛋白溶原激活剂的生产。 我们目前看到了备受争议的生物技术产业新时代的开端。现在自信的预测未来的发展前景还很难，但是这些多潜力的方法必将给我们将来制造生物产品带来很大的冲击。 for computer software engineering GB/T50314-2000 of electrical installations code for construction and acceptance of GBJ232- 92 intelligent building weak design construction figure set GJBT-471 2. system description this times engineering weak design including: hours a Kok information facilities system integrated wiring system (containing room network, communications part) wired TV system information guide and the released system hours II Kok public security system fire automatically alarm system (containing public broadcast part) security technology prevention system security monitoring system out entrance control system car library management system hours three Kok building equipment monitoring system hours four Kok information application system queued station ICU visitation system operation the system monitoring and training system call intercom system hours, five hours Kok Kok intelligent integrated system of information system (1) cabling systems (including computer room network, communication component) computer network systems and voice communications systems Center of information on medical floor computer room located in the engine room. Voice communication system using a local telecommunications operator providing voice service, configuration-independent integrated services digital program-controlled switchboards. Operators providing communications ... If any compatibility requirements, by way of increased access to the Communications Board within the chassis to meet