荧光素选择1

联科生物技术有限公司 各种荧光染料激发及发射波长 技术技术 www.gotofcm.com 电子邮件 service@gotofcm.com P r o b e E x ( n m ) Em (nm) M W N o t e s R e a c t i v e a n d c o n j u g a t e d p r o b e s Hydroxycoumarin 325 386 331 Succinimidyl ester Aminocoumarin 350 445 330 Succinimidyl ester Methoxycoumarin 360 410 317 Succinimidyl ester Cascade Blue 375;400 423 596 Hydrazide Lucifer yellow 425 528 NBD 466 539 294 NBD-X R-Phycoerythrin (PE) 480;565 578 240 k PE-Cy5 conjugates 480;565;650 670 aka Cychrome, R670, Tri-Color, Quantum Red PE-Cy7 conjugates 480;565;743 767 APC-Cy7 conjugates 650;755 767 PharRed Red 613 480;565 613 PE-Texas Red Fluorescein 495 519 389 FITC; pH sensitive FluorX 494 520 587 (AP Biotech) BODIPY-FL 503 512 TRITC 547 572 444 TRITC X-Rhodamine 570 576 548 XRITC Lissamine Rhodamine B 570 590 PerCP 490 675 Peridinin chlorphyll protein 联科生物技术有限公司 各种荧光染料激发及发射波长 技术技术 www.gotofcm.com 电子邮件 service@gotofcm.com Texas Red 589 615 625 Sulfonyl chloride Allophycocyanin (APC) 650 660 104 k TruRed 490,675 695 PerCP-Cy5.5 conjugate Alexa Fluor 350 346 445 410 (Molecular Probes) Alexa Fluor 430 430 545 701 (Molecular Probes) Alexa Fluor 488 494 517 643 (Molecular Probes) Alexa Fluor 532 530 555 724 (Molecular Probes) Alexa Fluor 546 554 570 1079 (Molecular Probes) Alexa Fluor 555 555 571 1250 (Molecular Probes) Alexa Fluor 568 578 602 792 (Molecular Probes) Alexa Fluor 594 590 617 820 (Molecular Probes) Alexa Fluor 633 621 639 1200 (Molecular Probes) Alexa Fluor 647 649 666 1250 (Molecular Probes) Alexa Fluor 660 668 698 1100 (Molecular Probes) Alexa Fluor 680 684 707 1150 (Molecular Probes) Cy2 489 506 714 (AP Biotech) Cy3 (512);550 570;(615) 767 (AP Biotech) Cy3.5 581 596;(640) 1102 (AP Biotech) Cy5 (625);650 670 792 (AP Biotech) Cy5.5 675 694 1128 (AP Biotech) 联科生物技术有限公司 各种荧光染料激发及发射波长 技术技术 www.gotofcm.com 电子邮件 service@gotofcm.com Cy7 743 767 818 (AP Biotech) N u c l e i c a c i d p r o b es Hoechst 33342 343 483 616 AT-selective DAPI 345 455 AT-selective Hoechst 33258 345 478 624 AT-selective SYTOX Blue 431 480 ~400 DNA Chromomycin A3 445 575 CG-selective Mithramycin 445 575 YOYO-1 491 509 1271 SYTOX Green 504 523 ~600 DNA SYTOX Orange 547 570 ~500 DNA Ethidium Bromide 493 620 394 7-AAD 546 647 7-aminoactinomycin D, CG-selective Acridine Orange 503 530/640 DNA/RNA TOTO-1, TO-PRO-1 509 533 Vital stain, TOTO: Cyanine Dimer TO-PRO: Cyanine Monomer Thiazole Orange 510 530 Propidium Iodide (PI) 536 617 668.4 TOTO-3, TO-PRO-3 642 661 LDS 751 543;590 712;607 472 DNA (543ex/712em), RNA (590ex/607em) 联科生物技术有限公司 各种荧光染料激发及发射波长 技术技术 www.gotofcm.com 电子邮件 service@gotofcm.com C e l l f u n c t i o n p r o b e s Indo-1 361/330 490/405 1010 AM ester. Low/High Ca++, Fluo-3 506 526 855 AM ester. pH > 6 DCFH 505 535 529 2'7'Dichorodihydrofluorescein, oxidized form DHR 505 534 346 Dihydrorhodamine 123, oxidized form, light catalyzes oxidation SNARF 548/579 587/635 pH 6/9 F l u o r e s c e n t P r o t e i n s Y66F 360 508 Y66H 360 442 EBFP 380 440 (Clontech) Quantum yield 0.18 Wild Type 396,475 508,503 GFPuv 385 508 (Clontech) ECFP 434 477 (Clontech) Quantum yield 0.40 Y66W 436 485 S65A 471 504 S65C 479 507 S65L 484 510 S65T 488 511 EGFP 489 508 (Clontech) Quantum yield 0.60 联科生物技术有限公司 各种荧光染料激发及发射波长 技术技术 www.gotofcm.com 电子邮件 service@gotofcm.com EYFP 514 527 (Clontech) Quantum yield 0.61 DsRed 558 583 (Clontech) Quantum yield 0.29 O t h e r p r o b e s Monochlorobimane 380 461 226 Glutathione probe Calcein 496 517 623 pH > 5