葡萄糖标准曲线

葡萄糖曲线 2.0葡萄糖标准曲线 1.8 1.6 1.4 1.2 1.0 吸光度0.8 0.6 0.4 0.2 0.0 0.00.20.40.60.81.01.21.41.61.82.0 葡萄糖含量/mg [2013/3/ 15:24 "/Graph1" (2456363)] Linear Regression through origin for Data1_B: Y = B * X Parameter Value Error ------------------------------------------------------------ A 0 -- B 0.80203 0.01276 ------------------------------------------------------------ R SD N P ------------------------------------------------------------ 0.9982 0.03646 9 <0.0001 ------------------------------------------------------------ 22Se氮源种类lsd=t*s s= 0.05x1-x2x1-x2n N=3 L0.05=45.877 L0.01=65.25334 氮源用量L=57.9393 L=82.4102 0.050.01 硫酸铵和豆饼粉含量L=36.0044 L=51.211 0.050.01硫酸铵和蛋白胨含量L=38.3182 L= 54.50201 0.050.01 Cβ-葡萄糖苷酶 x C 1 结晶纤维素 无定形纤维素 纤维二糖 葡萄糖 11 纤维素 CBHI、CBH II CBHI、CBH II 纤维糊精 纤维二糖 EGI、EG II BG 葡萄糖 因素 酶活值(U/g) 实验号 A B C D ? ? ? 平均值 1 1 1 1 1 276.502 259.698 291.778 275.992 2 1 2 2 2 301.122 251.622 348.146 300.297 3 1 3 3 3 267.357 253.553 244.109 255.006 4 2 1 2 3 238.445 248.778 294.082 260.435 5 2 2 3 1 332.598 291.779 302.362 308.913 6 2 3 1 2 288.606 398.204 405.511 364.107 7 3 1 3 2 377.461 383.599 329.895 363.652 8 3 2 1 3 444.975 436.250 438.841 440.022 9 3 3 2 1 477.430 446.196 423.886 449.171 900.081 1080.122 K831.296 0 1 1049.233 1009.904 K933.456 0 2 K1252.846 1068.285 927.572 0 3 K277.098 300.026 360.041 0 1T=3017.599 K349.744 336.634 311.152 0 2 K417.615 356.095 309.191 0 3 R 140.516 56.068 50.849 0 (2)正交实验结果 表16 正交实验结果及分析 Table16. Results and analysis of orthogonal test 因素 酶活值(U/g) 实验号 A B C D ? ? ? 平均值 1 1 1 1 275.992 1 276.502 259.698 291.778 300.297 2 1 2 2 2 301.122 251.622 348.146 255.006 3 1 3 3 3 267.357 253.553 244.109 4 2 1 2 260.435 3 238.445 248.778 294.082 5 2 2 3 308.913 1 332.598 291.779 302.362 364.107 6 2 3 1 2 288.606 398.204 405.511 7 3 1 3 363.652 2 377.461 383.599 329.895 8 3 2 1 440.022 3 444.975 436.250 438.841 9 3 3 2 449.171 1 477.430 446.196 423.886 K900.081 1080.122 0 831.296 1 K1049.233 1009.904 0 933.456 2 K 1252.846 1068.285 927.572 0 3 T=3017.599 K277.098 300.026 360.041 0 1 K349.744 336.634 0 311.152 2 K356.095 309.191 0 417.615 3 R 56.068 50.849 0 140.516 本项目以绿色木霉为菌种，对绿色木霉产纤维素酶固态发酵培养基配方中氮源进行选择 优化，提高产纤维素酶的活力。采用单因素实验分别研究了培养基生长氮源种类、生长氮源 用量对产纤维素酶活力的影响，再通过研究培养基中有机氮源和无机氮源的不同组合对产纤 维素酶活力的影响，最后通过正交实验三种不同氮源组合对培养基配方中氮源进行了优化， 以确定最优氮源组合。实验结果表明培养基配方中最优氮源组合为:硫酸铵为1.2%，豆饼 粉为0.4%，蛋白胨为0.4%。采用此培养基中最优氮源组合固态发酵培养绿色木霉产纤维素 酶活力达到448.558U/g。 The project is with green trichoderma viride strain, for green trichoderma viride produces cellulase nitrogen source in solid state fermentation culture medium formula selection optimization and enhance the vitality of cellulase production. Culture medium was studied by single factor experiment respectively growth nitrogen source type, dosage of nitrogen source on the influence of the cellulose enzyme activity, through the medium of the different combination of organic nitrogen source and inorganic nitrogen source to produce cellulose enzyme activity influence, finally through the orthogonal experiment of three different nitrogen source combination of nitrogen source in the culture medium formula has been optimized, in order to determine the optimum nitrogen source. Experiments showed that the optimal nitrogen source in the culture medium formula combination are: ammonium sulfate is 1.2%, bean cake powder is 0.4%, peptone 0.4%. Using the medium combination of the optimal nitrogen source in solid state fermentation culture green trichoderma viride produces cellulose enzyme activity reached 448.558 U/g. With the green trichoderma viride producing cellulose, the project selectes optimized nitrogen source of culture medium to enhance the vitality of cellulase production. Growth nitrogen source type and dosage of nitrogen source in culture medium is studied by experiment of single factor the influence of the cellulose enzyme activity,then the different combination of organic nitrogen source and inorganic nitrogen source of the culture medium is studied the influence of the cellulose enzyme activity which is produced by the green trichoderma viride. Finally,the optimized combination of organic nitrogen source and inorganic nitrogen source to produce the higher cellulose enzyme activity has been selected. Experiments showed that the optimal nitrogen source in the culture medium formula combination are: ammonium sulfate is 1.2%, bean cake powder is 0.4%, peptone 0.4%. The green trichoderma viride produces cellulose enzyme activity reached 448.558 U/g by using the combination of the optimal nitrogen source in solid fermentation culture medium. 本项目以绿色木霉为菌种，对绿色木霉产纤维素酶固态发酵培养基中氮源进行选择优化，以 提高纤维素酶活力。采用单因素实验对培养基中氮源种类、氮源浓度、两种氮源配比组合进 行了选择，再运用正交实验对三种氮源配比组合进行了优化。实验结果表明培养基中最优氮 源组合为硫酸铵1.2%、豆饼粉0.4%、蛋白胨0.4%。采用此最优氮源组合的培养基进行固态 发酵培养绿色木霉产纤维素酶活力达到448.558U/g。 This project is with green trichoderma viride strain, for green trichoderma viride produces cellulase choose nitrogen source in solid state fermentation medium optimization, in order to improve the cellulose enzyme activity. Using single factor experiment to medium kind of nitrogen source and nitrogen source concentration, ratio of two kind of nitrogen source combination selection, again with the orthogonal experiment of three nitrogen source matching combination is optimized. Experiments showed that the optimal nitrogen source in the culture medium composition for ammonium sulphate 1.2% 0.4%, peptone 0.4%, bean cake powder. Adopt the combination of the optimal nitrogen source of solid state fermentation culture medium for cultivating green trichoderma viride produces cellulose enzyme activity reached 448.558 U/g. With the green trichoderma viride producing cellulose, the project selectes optimized nitrogen source of culture medium to enhance the vitality of cellulase production. Nitrogen source type and nitrogen source concentration, ratio of two kind of nitrogen source combination were selected by using single factor experiment, then with the orthogonal experiment of three nitrogen source matching combination was optimized. The result of experiment showed that the optimal nitrogen source in the culture medium composition for ammonium sulphate 1.2% 0.4%, peptone 0.4%, bean cake powder. The combination of the optimal nitrogen source in solid state fermentation culture medium on trichoderma viride producing cellulose enzyme activity reached 448.558 U/g.